FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008151983

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008151983
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences644
Total Bases93.3 kbp
Sequences flagged as poor quality0
Sequence length142-145
%GC55

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACTTAAAAACAAGGCAGCTCTGAACGGTATTTCCTCTAAGACCAAGTCTC32951.08695652173913No Hit
ACTTAAAAACAAGGCAGTTCTGAACGGTATTTCCTCTAAGACCAAGTCTC10015.527950310559005No Hit
ACTTAAAAACAAGGCAGTGCTGAACGGTATTTCCTCTAAGACCAAGTCTC345.279503105590062No Hit
ACTTAAAAACAAGGCAGTGCTATCAACGAAGAGGTCCATTCTAGTGCCCC274.192546583850932No Hit
ACTTAAAACAAGGCAGCTCTGAACGGTATTTCCTCTAAGACCAAGTCTCT71.0869565217391304No Hit
ACTTAAAAACAAGGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGT50.7763975155279503Clontech SMARTer II A Oligonucleotide (96% over 25bp)
ACTTAAAAACAAGGCAGTGGTATCAACGCAGAGGTCCATTCTAGTGCCCC50.7763975155279503Clontech Universal Primer Mix Long (95% over 22bp)
ACTTAAAAACAAGGCAGTGGTATCAACGAAGAGGTCCATTCTAGTGCCCC50.7763975155279503No Hit
TGTGTGTGTGTTTCTGTGGGTAAGCAGTGGTATCAACGCAGAGTACATGG50.7763975155279503Clontech Universal Primer Mix Long (96% over 27bp)
ACTTAAAAACAAGGCAGCTCTGACGGTATTTCCTCTAAGACCAAGTCTCT40.6211180124223602No Hit
TGTGTGTGTGTTTCTGTGGCAGCTCTGAACGGTATTTCCTCTAAGACCAA40.6211180124223602No Hit
TGTGTGTGTGTTTCTGTGGGTTTCTTTAAGGTTTGGACAGAAGGGTAAAG40.6211180124223602No Hit
ACTTAAAAACAAGGCAGCTCTGAACGGTATTCCTCTAAGACCAAGTCTCT40.6211180124223602No Hit
ACTTAAAAACAAGGCAGTGTACATGGAAGCAGTGGTATCAACGCAGAGTA30.4658385093167702Clontech Universal Primer Mix Long (96% over 25bp)
ACTTAAAAACAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTA30.4658385093167702Clontech SMARTer II A Oligonucleotide (100% over 25bp)
ACTTAAAAACAAGGCAGTGCTATCAACGCAGAGTACATGAAGCAGTGGTA30.4658385093167702No Hit
ACTTAAAAACAAGGCAGTGCTAATGCCTAATGGTGCTACAGTTTCTGCCT30.4658385093167702No Hit
ACTTAAAAACAAGGCAGCTCTGAACGGTTTTCCTCTAAGACCAAGTCTCT20.3105590062111801No Hit
ACTTAAAAACAAGGCAGCTCTGAACGGTATTTCCTCTAAGACAAAGTCTC20.3105590062111801No Hit
TGTGTGTGTGTTTCTGTGGGTAAGCAGTGGTATCAACGCAGAGGTCCATT20.3105590062111801Clontech Universal Primer Mix Long (96% over 26bp)
ATTAAAAACAAGGCAGTTCTGAACGGTATTTCCTCTAAGACCAAGTCTCT20.3105590062111801No Hit
ACTTAAAAACAAGCAGTGGTATCAACGCAGAGTACATGGGAGCAGTGGTA20.3105590062111801Clontech SMARTer II A Oligonucleotide (100% over 25bp)
ACTTAAAAACAAGGCAGTGCTATCAACGCAGAGTACATGGGAAGCAGTGG20.3105590062111801No Hit
ACTTAAAAACAGGCAGTGCTATCAACGAAGAGGTCCATTCTAGTGCCCCT20.3105590062111801No Hit
ACTTAAAAACAAGGCAGTGCTACCGTATGGCAGCTCTGAACGGTATTTCC20.3105590062111801No Hit
ACTTAAAAACAAGGCAGTGCTATCAACGCAGAGGTCCATTCTAGTGCCCC20.3105590062111801No Hit
ACTTAAAAACAAGGCAGTGCTATCAACGCAGAGTACATGGAAGCAGTGGT20.3105590062111801No Hit
ACTTAAAAACAAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGT20.3105590062111801Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CTTAAAAACAAGGCAGTGCTATCAACGCAGAGTACATGGGGCAGTGGTAT10.15527950310559005Clontech Universal Primer Mix Long (95% over 21bp)
ACTTAAAACAAGGCAGTGCTATCAACGAAGAGGTCCATTCTAGTGCCCCT10.15527950310559005No Hit
CTTAAAAACAAGCAGTGGTATCAACGCAGAGTACATGGAAAGAGGGGGTT10.15527950310559005Clontech SMARTer II A Oligonucleotide (100% over 25bp)
ACTTAAAAACAAGGCAGTGCTGAACGGTATTCCTCTAAGACCAAGTCTCT10.15527950310559005No Hit
ACTTAAAAACAAGGCAGCTCTAACGGTATTTCCTCTAAGACCAAGTCTCT10.15527950310559005No Hit
TGTGTGTGTGTTTCTGTGGGCAGCTCTGAACGGTATTTCCTCTAAGACCA10.15527950310559005No Hit
ACTTAAAAACAAGGCAGCTCTGAACGTATTTCCTCTAAGACCAAGTCTCT10.15527950310559005No Hit
ACTTAAAAACAAGGCAGTGGTATCAACGAAGAGGTCCATTCTAGTGACCC10.15527950310559005No Hit
ACTTAAAGACAAGGCAGTTCTGAACGGTATTTCCTCTAAGACCAAGTCTC10.15527950310559005No Hit
ACTTAAAAACAAGGCAGTGCTATCAACGCAGAGTACATGAAGCAGTGATA10.15527950310559005No Hit
ACTTAAAAACAAGTCAGTTCTGAACGGTATTTCCTCTAAGACCAAGTCTC10.15527950310559005No Hit
ACTTAAAAACAAGGCAGTGCTATCAACGCAAAGTATATGGGAGCAGTGGT10.15527950310559005No Hit
ACTTAAAAACAAAGCAGTGGTATCAACGCAGAGTACATGGAGCAGTGGTA10.15527950310559005Clontech SMARTer II A Oligonucleotide (100% over 25bp)
ACTTAAAAACAAGGCAGCTCTGAACGGATTTCCTCTAAGACCAAGTCTCT10.15527950310559005No Hit
ACTTAAAAACAATGAAGCTCTGAACGGTATTTCATCTAAGACCAAGACTC10.15527950310559005No Hit
TGTGTGTGTGTTTCTGTGGGTAAGCAGTGGTATCAACGCAGAGGTCCACT10.15527950310559005Clontech Universal Primer Mix Long (96% over 26bp)
ACTTAAAACAAGGCAGTGCTAATGCTAATGGTGCTACAGTTTCTGCCTCT10.15527950310559005No Hit
ACTTAAAAACAAGGCAACTCTGAACGGTATTTCCTCTAAGACCAAGTCTC10.15527950310559005No Hit
ACTTAAACAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC10.15527950310559005Clontech SMARTer II A Oligonucleotide (100% over 25bp)
ACTTAAAAACAAGGCAGTGGTATCAACGCAGAGTACATAGAAGCAGTGGT10.15527950310559005Clontech SMARTer II A Oligonucleotide (96% over 25bp)
TGTGTGTGTGTTTCTGGCAGCTCTGAACGGTATTTCCTCTAAGACCAAGT10.15527950310559005No Hit
ACTTAAAAACAAGGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG10.15527950310559005Clontech SMARTer II A Oligonucleotide (96% over 25bp)
ACTTAAAAACAAGCAGTGGTATCAACGCAGAGTACATGAAGCAGTGGTAT10.15527950310559005Clontech SMARTer II A Oligonucleotide (100% over 25bp)
ACTTAAAAACAAGGCAGGTCTGAACGGTATTTCCTCTAAGACCAAGTCTC10.15527950310559005No Hit
ACTTAAAAACAAGGCAGTTCTAAACGGTATTTCCTCTAAGACCAAGTCTC10.15527950310559005No Hit
ACTTAAAAACATGGCAGCTCTGATCGGTATTTCCTCTAAGACCAAGTCTC10.15527950310559005No Hit
ACTTAAAAACAAGGCAGCTCTGAATGGTATTTCCTCTAAGACCAAGTCTC10.15527950310559005No Hit
ACTTAAAAACAAGGCAGTGGTATCAACGCAGGTCCATTCTAGTGCCCCTC10.15527950310559005No Hit
CTTAAAAACAAGGCAGTGTATCAACGCAGAGTACATGGAAGCGGTGGTAT10.15527950310559005No Hit
ACTTAAAAACAAGGCAGTGCTGAACGGTATTTCCTCTAAGACCAACTCTC10.15527950310559005No Hit
ACTTAAAAACAAGGCAGTGGTATCAACGCAGAGTACATGGGGCATTGGTA10.15527950310559005Clontech SMARTer II A Oligonucleotide (96% over 25bp)
ACGTAAAACAAGGCAGCTCTGAACGGTATTTCCTCTAAGACCAAGTCTCT10.15527950310559005No Hit
ACTTAAAAACAAGGCAGTGCTATCAACGCAGAGTACATGGGAGCAGTGGT10.15527950310559005No Hit
ACTTAAAAACAAGGCAGTGCTAACGGTATTTCCTCTAAGACCAAGTCTCT10.15527950310559005No Hit
ATTAAAAACAAGGCAGCTCTGAACGGTATTTCCTCTAAGACCAAGTCTCT10.15527950310559005No Hit
ACTTAAAAACAAGGCAGTGGTATCAACGCAAGGTCCATTCTAGTGCCCCT10.15527950310559005No Hit
ACTTAAAAACAAGGAGCTCTGAACGGTATTTCCTCTAAGACCAAGTCTCT10.15527950310559005No Hit
ATTAAAACAAGGCAGTGCTAATGCCTAATGGTGCTACAGTTTCTGCCTCT10.15527950310559005No Hit
TCTTAAAAACAAGGCAGTTCTGAACGGTATTTCCTCTAAGACCAAGTCTC10.15527950310559005No Hit
ACTAAAAACAAGGCAGTTCTGAACGGTATTTCCTCTAAGACCAAGTCTCT10.15527950310559005No Hit
ACTTAAAAACAAGGCAATGCTATCAACGAAAGGTCCATTCTAGTGCCCCT10.15527950310559005No Hit
ACTTAAAAATAAGGCAGCTCTGAACGGTATTTCCTCTAAGACCAAGTCTC10.15527950310559005No Hit
ACTTAAAAACAAGGCAGTGTACATGGGAAGCAGTGGTATCAACGCAGAGT10.15527950310559005Clontech Universal Primer Mix Long (100% over 23bp)
ACTTAAAACAAGGCAGTGCTGAACGGTATTTCCTCTAAGACCAAGTCTCT10.15527950310559005No Hit
ACTTAAAACAAGGCAGTTCTGACGGTATTTCCTCTAAGACCAAGTCTCTG10.15527950310559005No Hit
ACTTAAAAACAAGCAGTGGTATCAACGCAGAGTACAGGGAAGCAGTGGTA10.15527950310559005Clontech SMARTer II A Oligonucleotide (100% over 25bp)
ACTTAAAAACAAGGCGGCTCTGAACGGTATTTCCTCTAAGACCAAGTCTC10.15527950310559005No Hit
ACTTAAAAACAAGGCAGTTCTAACGGTATTTCCTCTAAGACCAAGTCTCT10.15527950310559005No Hit
ACTTAAAAACAAGGCAGCTCTGAACGGTATTACCTCTAAGACCAAGTCTC10.15527950310559005No Hit
ACTTAAAACAAGGCAGCTCTGAAGGTATTTCCTCTAAGACCAAGTCTCTG10.15527950310559005No Hit
ACTTAAAAACAAGGCAGTCTAACGGTATTTCCTCTAAGACCAAGTCTCTG10.15527950310559005No Hit
ACTAAAAACAAGGCAGTGCTACCGTATGGCAGCTCTGAACGGTATTTCCT10.15527950310559005No Hit
CTTAAAAACAAGGCAGCTCTGAACGGTATTTACTCTAAGACCAAGTCTCT10.15527950310559005No Hit
ACTTAAAAACAAGCAGTGCTATCAACGCAGAGTACATGGAAGCAGTGGTA10.15527950310559005Clontech SMARTer II A Oligonucleotide (96% over 25bp)
ACTTAAAAACAAGGCAGCTCTGTCCGCTATTACCTCAAAGACCAAGACAC10.15527950310559005No Hit
ACTTAAAAACAAGGCAGTGGTATCAACGCAGAGTACATGTATCCTTGGGA10.15527950310559005Clontech SMARTer II A Oligonucleotide (96% over 25bp)
ACTTAAAAACAAGGCAGTGGTATCAAACGCAGAGTACATGGAAGCAGTGG10.15527950310559005No Hit
ACTTAAAAACAAGGCAGTGCTACCGTAGGCAGCTCTGAACGGTATTTCCT10.15527950310559005No Hit
CTTAAAAACAAGGCAGCTCTGAACGGTATTTCCTCTAAGACCAAGTCTCT10.15527950310559005No Hit
ACTTAAAAACAAGGCAGCTCTGAACGGTATATCCTCTAAGACCAACTCTC10.15527950310559005No Hit
ACTCAAAAACAAGGCAGTTCTGAACGGTATTTCCTCTAAGACCAAGTCTC10.15527950310559005No Hit
ACTTAAAAACAAGGCAGTTCTGACGGTATTTCCTCTAAGACCAAGTCTCT10.15527950310559005No Hit
TGTGTGTGTGTTTCTGTGGGTAGCTCTGAACGGTATTTCCTCTAAGACCA10.15527950310559005No Hit
ACTTAAAAACGAGGCAGCTCTGAACGGTATTTCCTCTAAGACCAAGTCTC10.15527950310559005No Hit
CTTAAAACAAGGCAGTGGTATCACCGCAGAGTACATGGAAGCAGTGGTAT10.15527950310559005No Hit
ACTTAAAAACAAGGCAGCTCTGAACGGTATTTCCTCTAAGACCAACTCTC10.15527950310559005No Hit
ACTCAAAAACAAGGCAGTGGTATCAACGAAGAGGTCCATTCTAGTGCCCC10.15527950310559005No Hit
ACTTAAAAACAAGGCAGCTCTCAACGGTATTTCCTCTAAGACCAAGTCTC10.15527950310559005No Hit
TGTGTGTGTGTTTCTGTGGGTTTGGTCTAAGAGGTCCATTCTAGTGCCCC10.15527950310559005No Hit
ACTTAAAAACAAGGAAGTTCTGAACGGTATTTCATCTAAGACCAAGTCTC10.15527950310559005No Hit
AACTTAAAAACAAGGCAGCTCTGAACGGTATTTCCTCTAAGACCAAGTCT10.15527950310559005No Hit
ACTTAAAAACAAGGCAGTGGTATCAACGCAGAGTACATGAAGCAGTGGTA10.15527950310559005Clontech SMARTer II A Oligonucleotide (96% over 25bp)
ACTTAAAAACAAGGCAGCTCTGAACGGTATTTCCTCTAAGCCCAAGTCTC10.15527950310559005No Hit
ACTTAAAAACAGGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTA10.15527950310559005Clontech SMARTer II A Oligonucleotide (96% over 25bp)
ACTTAAGAACAAGGCAGCTCTGAACGGTATTTCCTCTAAGACCAAGTCTC10.15527950310559005No Hit
ACTTAAAAACAAAGCAGTGGTATCAACGCAGAGGTCCATTCTAGTGCCCC10.15527950310559005Clontech Universal Primer Mix Long (95% over 22bp)
TGTGTGTGTGTTTCTGTGGGCGGCTCTGAACGGTATTTCCTCTAAGACCA10.15527950310559005No Hit

[FAIL]Adapter Content

Adapter graph