FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008151984

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008151984
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences644
Total Bases93.3 kbp
Sequences flagged as poor quality0
Sequence length142-145
%GC59

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAATACCGTTCAGAGCTGCCTTGTTTTTAAGTTTACGCTGTAGAACCATG32750.77639751552795No Hit
AAATACCGTTCAGAACTGCCTTGTTTTTAAGTTTACGCTGTAGAACCATG9614.906832298136646No Hit
AAATACCGTTCAGCACTGCCTTGTTTTTAAGTTTACGCTGTAGAACCATG324.968944099378882No Hit
GGCACTAGAATGGACCTCTTCGTTGATAGCACTGCCTTGTTTTTAAGTTT264.037267080745342No Hit
GGCACTAGAATGGACCTCTTCGTTGATACCACTGCTTCCATGTACTCTGC111.7080745341614907Clontech SMARTer II A Oligonucleotide (95% over 22bp)
AAATACCGTTCAGAGCTGCCTTGTTTTAAGTTTACGCTGTAGAACCATGT71.0869565217391304No Hit
GGCACTAGAATGGACCTCTTCGTTGATACCACTGCCTTGTTTTTAAGTTT60.9316770186335404No Hit
GGCACTAGAATGGACCTCTGCGTTGATACCACTGCCTTGTTTTTAAGTTT50.7763975155279503Clontech SMARTer II A Oligonucleotide (95% over 22bp)
GGCACTAGAATGGACCTCTGCGTTGATACCACTGCTCCCATGTACTCTGC40.6211180124223602Clontech Universal Primer Mix Long (95% over 22bp)
AATACCGTTCAGAGCTGCCTTGTTTTTAAGTTTACGCTGTAGAACCATGT40.6211180124223602No Hit
AAATACCGTCAGAGCTGCCTTGTTTTTAAGTTTACGCTGTAGAACCATGT40.6211180124223602No Hit
AAATACCGTTCAGAGCTGCCACAGAAACACACACACATTACGCTGTAGAA40.6211180124223602No Hit
GGCACTAGAATGGACCTCTTCGTTGATACCACTGCTCCATGTACTCTGCG30.4658385093167702Clontech SMARTer II A Oligonucleotide (95% over 21bp)
GGCACTAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATAC30.4658385093167702No Hit
GGCACTAGAATGGACCTCTGCGTTGATACCACTGCTTCCATGTACTCTGC30.4658385093167702Clontech Universal Primer Mix Long (95% over 23bp)
GGCACTAGAATGGACCTCTTCGTTGATACCACTGCTCCCATGTACTCTGC30.4658385093167702Clontech SMARTer II A Oligonucleotide (95% over 21bp)
AAATACCGTTCAGAGCTGCCTTGTTTTTAAGTTTACGCTGTAGCACCATG20.3105590062111801No Hit
GGCACTAGAATGGACCTCTTCGTTGATACCACTGCCCCATGTACTCTGCG20.3105590062111801No Hit
AAATACCGTTCAGAACTGCCTTGTTTTTAATTTACGCTGTAGAACCATGT20.3105590062111801No Hit
GGCACTAGAATGGACCTCTTCGTTGATACCACTGCTTCCCATGTACTCTG20.3105590062111801Clontech SMARTer II A Oligonucleotide (95% over 22bp)
AAATACCGTTCAGAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGAACC20.3105590062111801No Hit
GGCACTAGAATGGACCTCTGCGTTGATACCACTGCTTACCCACAGAAACA20.3105590062111801Clontech Universal Primer Mix Long (95% over 23bp)
AAATACCGTTCAGAGCTGCCATACGGTAGCACTGCCTTGTTTTTAAGTTT20.3105590062111801No Hit
GGCACTAGAATGGACCTCTGCGTTGATACCACTGCTTCATGTACTCTGCG20.3105590062111801Clontech Universal Primer Mix Long (95% over 23bp)
AAATACCGTTCAGAGCTGCCTTGTTTTTAAGTTACGCTGTAGAACCATGT20.3105590062111801No Hit
AAAACCGTTCAGAGCTGCCTTGTTTTTAAGTTTACGCTGTAGAACCATGT20.3105590062111801No Hit
GGCACTAGAATGGACCTCTTCGTTGATACCACTGCTTCATGTACTCTGCG20.3105590062111801Clontech SMARTer II A Oligonucleotide (95% over 22bp)
GGCACTAGAATGGACCTCTTCGTTGATAGCACTGCCTGTTTTTAAGTTTA20.3105590062111801No Hit
GGCACTAGAATGGACCTCTGCGTTGATAGCACTGCCTTGTTTTTAAGTTT20.3105590062111801No Hit
GGCACTAGAATGGACCTCTGCGTTGATACCACTGCTGCCATGTACTCTGC10.15527950310559005Clontech Universal Primer Mix Long (95% over 22bp)
AAATACCGTCAGAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGAACCT10.15527950310559005No Hit
GGCACTAGAATGGACCTCTTCGTTGATACCACTGCTTCCCTGTACTCTGC10.15527950310559005Clontech SMARTer II A Oligonucleotide (95% over 22bp)
AAATACCGTTCAGAACTGCCTTGTTTTTAAGTTTACGCTGTGGAACCATG10.15527950310559005No Hit
AAATACCGTTCAGAGCTGCCATACGGTAGCACTGCCTTGTTTTTAGTTTA10.15527950310559005No Hit
AAATACCGTTCAGAACTGCCTTGTTTTTAAGTTTACGCCGTAGAACCATG10.15527950310559005No Hit
AAATACCGTTCAGAACTGACGAGTTTTTAAGTTTACGATCAAGAACCATC10.15527950310559005No Hit
AAATACCGTTCAGAGCTGCCTTATTTTTAAGTTTACGCTGTAGAACCATG10.15527950310559005No Hit
GGCACTAGAATGGACCTCTTCGTTGATAGCACTGCCTTGTATTTAAGTTT10.15527950310559005No Hit
AAATACCGTTCAGAGCTGCCTCGTTTTTAAGTTTACGCTGTAGAACCATG10.15527950310559005No Hit
AAATACCGTTCAGAGCTGCCACCAAGAGCTTGCAAATTGCTGCTGGAACC10.15527950310559005No Hit
AAATACCGTTCAGAACTGCCTTGTTTTTAAGATTACGCTGTAGAACCATG10.15527950310559005No Hit
AAATACCGTTCAGAGCTGCCACCAGGAGCTTGCAAGTTGCTGCTGGAACC10.15527950310559005No Hit
AAATACCGTTCAGAGCTGCCTAGTTTTTAAGTTTAAGCTGTAGAACCATG10.15527950310559005No Hit
AAATACCGTTCAGAACTGCCTTGTTTTTGAGTTTACGCTGTAGAACCATG10.15527950310559005No Hit
AAATACCGTTAGACTGCCTTGTTTTTAAGTTTACGCTGTAGAACCATGTC10.15527950310559005No Hit
AAATACCGTTNNGAGCTGCCTTGTTTTTAAGTTTACGCTGTAGAACCATG10.15527950310559005No Hit
AAATACCGTTCAGAACTGCCTTGTCTTTAAGTTTACGCTGTAGAACCATG10.15527950310559005No Hit
GGCACTAGAATGGACCTCTGCGTTGATACCACTGCTTTGTTTTTAAGTTT10.15527950310559005Clontech Universal Primer Mix Long (95% over 23bp)
AAATACCTTCAGAGCTGCCTTGTTTTAAGTTTACGCTGTAGAACCATGTC10.15527950310559005No Hit
GGCACTAGAATGGACCTCTGCGTTGATACCACTGATTCCATGTACTAAGC10.15527950310559005Clontech SMARTer II A Oligonucleotide (95% over 22bp)
AATACCGTTCAGCACTGCCTTGTTTTTAAGTTTACGCTGTAGAACCATGT10.15527950310559005No Hit
GCACTAGAATGGACCTCTTCGTTGATACCACTGCTTCCATGTACTCTGCG10.15527950310559005Clontech SMARTer II A Oligonucleotide (95% over 22bp)
AAATACCGTTCAGAGTTGCCTTGTTTTTAAGTTTACGCTGTAGAACCATG10.15527950310559005No Hit
AAATACCGTTCAGAGCTGCCTTGTTTTTAAGTTTACGCTGTAGAGCCATG10.15527950310559005No Hit
AAATACCATTCAGAGCTGCCTTGTTTTTAAGTTTACGCTGTAGAACCATG10.15527950310559005No Hit
AAATACCGTTCAGAACTGACTTGTTTTTAAGTTTACGCTGTAGAACCATG10.15527950310559005No Hit
GGCACTAGAATGGACCTCTTCGTTGATACCACTGCTTCTATGTACTCTGC10.15527950310559005Clontech SMARTer II A Oligonucleotide (95% over 22bp)
GGCACTAGAATGGACCTTGCGTTGATACCACTGCCTTGTTTTTAAGTTTA10.15527950310559005No Hit
GGCACTAGAATGACCTCTTCGTTGATACCACTGCTTCCATGTACTCTGCG10.15527950310559005Clontech SMARTer II A Oligonucleotide (95% over 22bp)
AAATACCGTTCAGAGCTGCCTTGTTTTTAAGTATACGCTGTAGAACCATG10.15527950310559005No Hit
AAATACCGTTCAGAGCTACCCACAGAAACACACACACATTACGCTGTAGA10.15527950310559005No Hit
AAATACCGTTCAGAACTGCCTTGTTTTTAAGTTTACGCTGTAGACCCATG10.15527950310559005No Hit
AAATACCGTTTAGAACTGCCTTGTTTTTAAGTTTACGCTGTAGAACCATG10.15527950310559005No Hit
AAATACCGTTCAGAGCTGCCTTGTTTTACGTTTACGCTGTAGAACCATGT10.15527950310559005No Hit
AAATACCGTTCAGAGCCGCCCACAGAAACACACACACATTACGCTGTAGA10.15527950310559005No Hit
AAATACCGTTCAGAACTGCCTTGTTTTAAGTTTACGCTGTAGAACCATGT10.15527950310559005No Hit
AAATACCGTTCAGAGCTGCCTACGGTAGCACTGCCTTGTTTTTAAGTTTA10.15527950310559005No Hit
GGCACTAGAATGGACCTCTTCGTTGATACCACTGCTACATTTTAACTTCG10.15527950310559005Clontech SMARTer II A Oligonucleotide (95% over 21bp)
AAATACGTTCAGAGCTGCCTTGTTTTTAAGTTTACGCTGTAGAACCATGT10.15527950310559005No Hit
AAATACCGTTGAGAGCTGCCTTGTTTTTAAGTTTACGCTGTAGAACCATG10.15527950310559005No Hit
AAATACCGTTCAGCACTGCCTTGTTTTTAAGTTTACTCTGTAGAACCATG10.15527950310559005No Hit
AAATACCGTTCAGAGCTCCTTGTTTTTAAGTTTACGCTGTAGAACCATGT10.15527950310559005No Hit
AAATACCGTTCAGAGCTGCCTTGTTTTTAAGTTTTACGCTGTAGAACCAT10.15527950310559005No Hit
GGCACTAGAATGGACCTCTTCGTTGATACCACTGCCTTGTTTTTGAGTTT10.15527950310559005No Hit
GGCACTAGAATGGACCTCTGCGTTGATACCACTGCTCCATGTACTCTGCG10.15527950310559005Clontech Universal Primer Mix Long (95% over 22bp)
GGCACTAGAATGGACCTCTTAGACCAAACCCACAGAAACACACACACATT10.15527950310559005No Hit
AAATACCGTTCAGAGCTGCCTTGTTTTTAAGTTTACGCTGGAGAACCATG10.15527950310559005No Hit
GGCACTAGAATGGACCTCTGCGTTGATACCCCTGCTCCCATGTACTCTGC10.15527950310559005Clontech SMARTer II A Oligonucleotide (95% over 21bp)
AAATACCGTTCAGCACTGCCTTGTTTTAAGTTTACGCTGTAGAACCATGT10.15527950310559005No Hit
AAATACCGTTCAGAGCTGCCTTGTTTTTAAGTTTACGCTGTAGAAACATG10.15527950310559005No Hit
AAATACCGTTCAGCACTGCCTTGTTTTTAAGTTTACGCTGTAGATCCATG10.15527950310559005No Hit
AAATACCGTTAAGACCTCAGTTGTTTTAAAGGTTACGATGTATAAACATG10.15527950310559005No Hit
AAATACCGTTCAGAGCTGCCTTGTTTTTAATTTACGCTGTAGAACCATGT10.15527950310559005No Hit
AAATACCGTTCAGAGCTGCCTGGTTTATAAGTTTACGCTGTAGAACCATG10.15527950310559005No Hit
AAATACCGTTCAGAACTGCCTTGTGGTTAAGTTTACGCTGTAGAACCATG10.15527950310559005No Hit
AAATACCGTCAGAACTGCCTTGTTTTAAGTTTACGCTGTAGAACCATGTC10.15527950310559005No Hit
AAATACCGTTCAGAGCCGCCTTGTTTTTAAGTTTACGCTGTAGAACCATG10.15527950310559005No Hit
AAATACCGTTCAGAGCTGCCCACAGAAACACACACACATTACGCTGTAGA10.15527950310559005No Hit
GGCACTAGAATGGACCTCTTCGTTGATAGCACTGCCTTGTTTTAAGTTTA10.15527950310559005No Hit
AAATACCGTTAGCACTGCCTTGTTTTTAAGTTTACGCTGTAGAACCATGT10.15527950310559005No Hit
AAATACCGTTCAGAGCTGCCTTGTTCTTAAGTTTACGCTGTAGAACCATG10.15527950310559005No Hit
GGCACTAGAATGGACCTCTTCGTTGATACCACTGTTTCCATGTACTCTGC10.15527950310559005No Hit
AAATACCGTCAGAACTGCCTTGTTTTTAAGTTTACGCTGTAGAACCATGT10.15527950310559005No Hit
GGCACTAGATGGACCTCTTCGTTGATACCACTGCTTCCATGTACTCTGCG10.15527950310559005Clontech SMARTer II A Oligonucleotide (95% over 22bp)
GGCACTAGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATAC10.15527950310559005No Hit
AAATACCGTTAGAACTGCCTTGTTTTTAAGTTTACGCTGTAGAACCATGT10.15527950310559005No Hit
AAATACCGTTCAGAGCTGCCATGTTTTTAAGTTTACGCTGTAGAACCATG10.15527950310559005No Hit
GGCACTAGAATGACCTCTGCGTTGATACCACTGCTTCCATGTACTCTGCG10.15527950310559005Clontech Universal Primer Mix Long (95% over 23bp)
AAATCCGTTCAGAGCTGCCTTGTTTTTAAGTTTACGCTGTAGAACGATGT10.15527950310559005No Hit
AAATACCGTTAGAGCTGCCTTGTTTTTAAGTTTACGCTGTAGAACCATGT10.15527950310559005No Hit
GGCACTAGAATGGACCTCTTCGTTGATACCACTGCCCCCATGTACTCTGC10.15527950310559005No Hit
GGCACTAGAATGGAACTCTTATTTGATACACATGAGTCCATGTACGCTTC10.15527950310559005No Hit
AAATACCGTTCAGCACTGCCTTGTTTTTAAGTTTACGCTGTAGGACCATG10.15527950310559005No Hit
GGCACTAGAATGGACCTTTCGTTGATAGCATTGCCTTGTTTTTAAGTTTA10.15527950310559005No Hit
AAATACCGTTCAGAGCTGCCAGAAACACACACACATTACGCTGTAGAACC10.15527950310559005No Hit
GGCACTAGAATGGACCTGCGTTGATACCACTGCCTTGTTTTTAAGTTTAC10.15527950310559005No Hit
GGCACTAGAGTGGACCTCTGCGTTGATACCACTGCTTACCCACAGAAACA10.15527950310559005Clontech Universal Primer Mix Long (95% over 23bp)
AAATACCGTTCAGACCTGCCTTGTTTTTAAGTTTACGCTGTAGAACCATG10.15527950310559005No Hit
GGCACTAGAATGGACCTCTGCGTTGATACCACTGCTTCCCATGTCCTCTC10.15527950310559005Clontech Universal Primer Mix Long (95% over 23bp)

[FAIL]Adapter Content

Adapter graph