FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008151985

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008151985
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences512
Total Bases74.2 kbp
Sequences flagged as poor quality0
Sequence length142-145
%GC56

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACTTAAAAACAAGGCAGCTCTGAACGGTATTTCCTCTAAGACCAAGTCTC22143.1640625No Hit
ACTTAAAAACAAGGCAGTTCTGAACGGTATTTCCTCTAAGACCAAGTCTC8015.625No Hit
ACTTAAAAACAAGGCAGTGCTGAACGGTATTTCCTCTAAGACCAAGTCTC377.2265625No Hit
ACTTAAAAACAAGGCAGTGCTATCAACGAAGAGGTCCATTCTAGTGCCCC285.46875No Hit
ACTTAAAAACAAGGCAGTGGTATCAACGAAGAGGTCCATTCTAGTGCCCC91.7578125No Hit
ACTTAAAAACAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTA91.7578125Clontech SMARTer II A Oligonucleotide (100% over 25bp)
TGTGTGTGTGTTTCTGTGGCAGCTCTGAACGGTATTTCCTCTAAGACCAA81.5625No Hit
TGTGTGTGTGTTTCTGTGGGTAAGCAGTGGTATCAACGCAGAGTACATGG50.9765625Clontech Universal Primer Mix Long (96% over 27bp)
ACTTAAAAACAAGGCAGTGCTATCAACGCAGAGTACATGGAAGCAGTGGT50.9765625No Hit
ACTTAAAAACAAAGCAGTGGTATCAACGAAGAGGTCCATTCTAGTGCCCC40.78125Clontech Universal Primer Mix Long (95% over 22bp)
ACTTAAAACAAGGCAGCTCTGAACGGTATTTCCTCTAAGACCAAGTCTCT40.78125No Hit
ACTTAAAAACAAGGCAGTGCTATCAACGCAGAGTACATGGGAAGCAGTGG30.5859375No Hit
ACTTAAAAACAAGGCAGTGCTACCGTAGGCAGCTCTGAACGGTATTTCCT30.5859375No Hit
ACTTAAAAACAAGGCAGCTCTAACGGTATTTCCTCTAAGACCAAGTCTCT20.390625No Hit
ACTTAAAAACAAGGTAGCTCTGAACGGTATTTCCTCTAAGACCAAGTCTC20.390625No Hit
ACTTAAAAACAAGGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGT20.390625Clontech SMARTer II A Oligonucleotide (96% over 25bp)
ACTTAAAAACAAGGCAGTGGTATCAACGCAGAGGTCCATTCTAGTGCCCC20.390625Clontech Universal Primer Mix Long (95% over 22bp)
ACTTAAAAACAAGGCAGTGCTATCAACGAAGAGGTCCATTCTAGTGACCC20.390625No Hit
CTTAAAAACAAGGCAGCTCTGAACGGTATTTCCTCTAAGACCAAGTCTCT20.390625No Hit
TGTGTGTGTGTTTCTGTGGGTAAGCAGTGGTATCAACGAAGAGGTCCATT20.390625Clontech SMARTer II A Oligonucleotide (95% over 22bp)
ACTTAAAAACAAGGCAGCTCTGAACGGTATTCCTCTAAGACCAAGTCTCT20.390625No Hit
ACTTAAAAACAAGGCAGTGCTATCAACGCAGAGGTCCATTCTAGTGCCCC20.390625No Hit
ACTTAAAAACAAGGCAGCTCTGAACGGTTTTCCTCTAAGACCAAGTCTCT10.1953125No Hit
ACTTAAAAACAAGGCAGTGGTATCAACGCAGAGTACATGGGGCAGTGGTA10.1953125Clontech SMARTer II A Oligonucleotide (96% over 25bp)
ACTCAAAAACAAGGCAGTGCTATCAACGAAGAGGTCCATTCTAGTGCCCC10.1953125No Hit
TGTGTGTGGTTTCAACGCAGAGTACTTGGAAGCAGTGGTATCAACGCAGA10.1953125Clontech Universal Primer Mix Long (100% over 21bp)
ACTTAAAAACAGGCAGCTCTGAACGGTATTTCCTCTAAGACCAAGTCTCT10.1953125No Hit
TGTGTGTGGGTTTCTGTGCCCCTCTAAGACCAAGTCTCTGCTACCGTAAT10.1953125No Hit
TGTGTGTGTGTTTCTGTGGGCAGCTCTGAACGGTATTTCCTCTAAGACCA10.1953125No Hit
ACTTAAAAACAAGGCAGCTCTGAACGTATTTCCTCTAAGACCAAGTCTCT10.1953125No Hit
CTAAAAACAAGCAGTGGTATCAACGCAGAGTACATGGCAAGAAGTGGTAT10.1953125Clontech SMARTer II A Oligonucleotide (100% over 25bp)
ACTTAAAAACAAGGCAGCTCTGAACGGTATTTCCTCTAAGACAAAGTCTC10.1953125No Hit
ACTTAAAAAACAAGGCAGTTCTGAACGGTATTTCCTCTAAGACCAAGTCT10.1953125No Hit
ACTTAAAAACAAGGCAGTGCTATCAACGAAGAGGTCCATTCTGTGCCCCT10.1953125No Hit
CTTAAAAACAAGGCAGTGCTACCGTAGGCAGCTCTGAACGGTATTTCCTC10.1953125No Hit
ACTTAAAAACAAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG10.1953125Clontech SMARTer II A Oligonucleotide (100% over 25bp)
ACTTAAAAACAAGGCAGCTCTGACGGTATTTCCTCTAAGACCAAGTCTCT10.1953125No Hit
ACTTAAAAACAAGACAGTGCTATCAACGAAGAGGTCCATTCTAGTGCCCC10.1953125No Hit
AGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAG10.1953125Clontech SMARTer II A Oligonucleotide (100% over 24bp)
TGTGTGTGTGTTTCTGTGGGTAAGCAGTGGTATCAACGCAGAGTTCCATT10.1953125Clontech Universal Primer Mix Long (96% over 27bp)
ACTTAAAAACAAGGCAGTGGTATCAACGGAGAGGTCCATTCTAGTGCCCC10.1953125No Hit
ACTTAAAAACAAGGCAGCGCTGAACGGTATTTCCTCTAAGACCAAGTCTC10.1953125No Hit
ACTTAAAAACAAGGCAGTCTGAACGGTATTCCTCTAAGACCAAGTCTCTG10.1953125No Hit
GTGTGTGTGTTTCTGTGGGTAAGCAGTGGTATCAACGCAGAGTACATGGA10.1953125Clontech Universal Primer Mix Long (96% over 27bp)
ACTTAAAAACAAGGCAGTGGTATCAACGCAGAGTTCCATTCTAGTGCCCC10.1953125Clontech Universal Primer Mix Long (95% over 23bp)
ACTTAAAAACAAGCAGTGGTATCAACGCAGAGTACATGAAGCAGTGGTAT10.1953125Clontech SMARTer II A Oligonucleotide (100% over 25bp)
ACTTAAAAACAAGGCAGCTCTGAAGGTATTTCCTCTAAGACCAAGTCTCT10.1953125No Hit
ACTTAAAAACAAGCCAGCTCTGAACGGTATTTCCTCTAAGACCAAGTCTC10.1953125No Hit
ACTTAAAAACAAGCAGTGCTATCAACGAAGAGGTCCATTCTAGTGCCCCT10.1953125No Hit
ACTTAAAAACAAGGCAGCTCTGAACGGTATTTCCTCTAAGAACAAGTCTC10.1953125No Hit
ACTTAAACAAGGCAGTGCTATCAACGAAGAGGTCCATTCTAGTGCCCCTC10.1953125No Hit
ACAAAAAAACAAGGCAGTTCTGAACGCTATTTCCTCTAAGACCAAGACTC10.1953125No Hit
ACTTAAAAACAAGCAGTGGTATCAACGCAGAGGTCCATTCTAGTGCCCCT10.1953125Clontech Universal Primer Mix Long (95% over 24bp)
ACTTAAAAACAAAGCAGTGGTATCAACGCAGAGTACATGGGAGCAGTGGT10.1953125Clontech SMARTer II A Oligonucleotide (100% over 25bp)
ACTTAAAAACAAGGCAGTTCTCAACGCTAATTCCTCTAAGACCAACACTC10.1953125No Hit
ATTAAAAACAAGGCAGCTCTGAACGGTATTTCCTCTAAGACCAAGTCTCT10.1953125No Hit
ACTTAAAAACAAGGCAGTGCTAACGGTATTTCCTCTAAGACCAAGTCTCT10.1953125No Hit
ACTTAAAAACAAGGCAGCTCTGATCGGTATTTCCTCTAAGACCAAGTCTC10.1953125No Hit
TCTTAAAAACAAGGCAGTTCTGAACGGTATTTCCTCTAAGACCAAGTCTC10.1953125No Hit
TGTGTGTGTGTTTCTGCGGGTTGGGGTAGTGGTCTTGGGGTAGTGGTGGA10.1953125No Hit
ACTTAAAAACAAGGCAGCTTGAACGGTATTTCCTCTAAGACCAAGTCTCT10.1953125No Hit
ACTTAAAAACAAGGCAGCTCTGGACGGTATTCCTCTAAGACCAAGTCTCT10.1953125No Hit
ACTTAAAACAAGGCAGCTCTGAACGTATTTCCTCTAAGACCAAGTCTCTG10.1953125No Hit
ACTTAAAAACAAGGCAGTTCTGAACGGTATTTCCTCTAAGCCCAAGTCTC10.1953125No Hit
ACTTAAAAACAAGGCAGTGATATCAACGAAGAGGTCCATTCTAGTGCCCC10.1953125No Hit
ACTTAAAAACAAGCAGTGGTATCAACGAAGAGGTCCATTCTAGTGCCCCT10.1953125Clontech SMARTer II A Oligonucleotide (95% over 22bp)
ACTTAAAAACAAGCAGTGGTATCAACGCAGAGTACATGGGAGCAGTGGTA10.1953125Clontech SMARTer II A Oligonucleotide (100% over 25bp)
ACTTAAAAACAAGGCAGTCTGAACGGTATTTCCTCTAAGACCAAGTCTCT10.1953125No Hit
ACTTAAAAACAAGGCGGCTCTGAACGGTATTTCCTCTAAGACCAAGTCTC10.1953125No Hit
ACTTAAAAACAAGGCAGTGTTATCAACGCAGAGTACATGGAAGCAGTGGT10.1953125No Hit
CTTAAAAACAAGGCAGTTCTGAACGGTATTTCCTCTAAGACCAAGTCTCT10.1953125No Hit
ACTTAAAAACAAGGCAGCTCTGAACGGTATTACCTCTAAGACCAAGTCTC10.1953125No Hit
ACTTAAAAACAAGTCAGTCTGAACGGTATTTCCTCTAAGACCAAGTCTCT10.1953125No Hit
ACTTAAAAACAAGCAGTGGTATCAACGCAGAGTACATGGGGCAGTGGTAT10.1953125Clontech SMARTer II A Oligonucleotide (100% over 25bp)
TGTGTGTGTGTTTCTGTGGGAGCAGTGGTATCAACGAAGAGGTCCATTCT10.1953125No Hit
ACTTAAAAACGAGGCAGTGCTGAACGGTATTTCCTCTAAGACCAAGTCTC10.1953125No Hit
ACTTAAAAACAAGGCAGTGCTGAACGGATTTCCTCTAAGACCAAGTCTCT10.1953125No Hit
TGTGTGTGTGTTTCTGTGGGAAGCAGTGGTATCAACGCAGAGTACATGAA10.1953125Clontech Universal Primer Mix Long (96% over 26bp)
TGTGTGTGTGTTTCTGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTA10.1953125No Hit
ACTTAAAAACAAGCAGTGCTGAACGGTATTTCCTCTAAGACCAAGTCTCT10.1953125No Hit
ACTTAAAAACAAGGCAGTTCTGAACGGTATTTCCTCTAAGACCATGTCTC10.1953125No Hit
ACTTAAAAACAAGGCAGTGCTACCGTATGGCAGCTCTGAACGGTATTTCC10.1953125No Hit
ACTTAAAAACAAGGCAGTGGTATCAACGCAGAGTACATGGGAGCAGTGGT10.1953125Clontech SMARTer II A Oligonucleotide (96% over 25bp)
ACTTAAAAACAAGGCAGTGCTACCGTGGCAGCTCTGAACGGTATTTCCTC10.1953125No Hit
TGTGTGTGTGTTTCTGTGGGTAGCTCTGAACGGTATTTCCTCTAAGACCA10.1953125No Hit
ACTTAAAACAAGGCGGCTCTGAACGGTATTTCCTCTAAGACCAAGTCTCT10.1953125No Hit
TGTGTGTGTGTTTCTGTGGCAGCTCTGAACCGGTATTTCCTCTAAGACCA10.1953125No Hit
ACTTAAAAACAAGGAAGTGGTATCAACGCAGAGTTGCAGTCATTGGTCCG10.1953125No Hit
ACTTAAAAACATGGCAGCTCTGAACGGTATTTCCTCTAAGACCAAGTCTC10.1953125No Hit
ACTTAAAAACAAGGCAGTGCTAATGCCTAATGGTGCTACAGTTTCTGCCT10.1953125No Hit
CTTAAAAACAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTAT10.1953125Clontech SMARTer II A Oligonucleotide (100% over 25bp)
ACTTAAAACCAAGGAGTGCTATCAACGAAGAGGTCCATTCTAGTGCCCCT10.1953125No Hit
TGTGTGTGTGTTTCTGTGGATAAGCAGTGGTATCAACGCAGAGTACATGG10.1953125Clontech SMARTer II A Oligonucleotide (100% over 25bp)
ACTTAAAAACAAGGCAGTGGTATCAACGCAGAGTACATGAAGCAGTGGTA10.1953125Clontech SMARTer II A Oligonucleotide (96% over 25bp)
ACTTAAAAACAAGGCAGCTCTGAACGGTATTTCCTCTAAGCCCAAGTCTC10.1953125No Hit
ATTAAAAACAAGGCAGTGCTATCAACGAAGAGGTCCATTCTAGTGCCCCT10.1953125No Hit
ACTTAAAAACAAAGCAGTGGTATCAACGCAGAGGTCCATTCTAGTGCCCC10.1953125Clontech Universal Primer Mix Long (95% over 22bp)
ACTTAAAAACAAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGT10.1953125Clontech SMARTer II A Oligonucleotide (100% over 25bp)
ACTTAAACACAAGGCAGCTCGAACGGTATTTCCTCTAAGACCAAGTCTCT10.1953125No Hit
ACTTAAAAACAAGGCAGCTCGAACGGTATTTCCTCTAAGACCAAGTCTCT10.1953125No Hit

[FAIL]Adapter Content

Adapter graph