FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008151990

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008151990
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12677
Total Bases1.8 Mbp
Sequences flagged as poor quality0
Sequence length140-145
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAATACCGTTCAGAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGAACC379029.896663248402618No Hit
GGCACTAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATAC327625.842076200993922No Hit
GGCACTAGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATAC194715.358523309931371No Hit
AAATACCGTTCAGAGCTGCCTTGTTTTTAAGTTTACGCTGTAGAACCATG5524.3543425100575845No Hit
AAATACCGTTCAGAACTGCCTTGTTTTTAAGTTTACGCTGTAGAACCATG1401.1043622308117063No Hit
AAATACCGTTCAGAGCTGCCAGCTTGGGGTACCTGGGAGGGCACAGCACT1120.8834897846493651No Hit
AAATACCGTTCAGAGCTGCCAGCTTGGGGTACCTGGGAGGGCATAGCACT1010.7967184665141595No Hit
GCACTAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA900.709947148378954No Hit
GGCACTAGATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA870.6862822434329889No Hit
AAATACCGTTCAGCACTGCCTTGTTTTTAAGTTTACGCTGTAGAACCATG620.48907470221661276No Hit
GCACTAGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA530.4180799873787173No Hit
GGCACTGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA480.37863847913544213No Hit
AATACCGTTCAGAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGAACCT440.34708527254082194No Hit
GGCACTAGAATGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA430.3391969708921669No Hit
GGCACTAGATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA360.2839788593515816No Hit
GGACTAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA330.26031395440561644No Hit
GGCACTAAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA320.25242565275696144No Hit
GGCATAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA300.23664904945965132No Hit
AAATACGTTCAGAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGAACCT290.22876074781099628No Hit
AAATACCGTTCAGAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGACCC270.21298414451368622No Hit
GGCACTAGAATGGACCTCTTCGTTGATAGCACTGCCTTGTTTTTAAGTTT270.21298414451368622No Hit
GGCACTAGAATGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA250.19720754121637613No Hit
GGCACTGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA250.19720754121637613No Hit
GGCACTAGAATGGACTCTTCCTCATCACTACATTATTTCAATTAAATACA240.18931923956772106No Hit
AAATACCGTTCAAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGAACCT240.18931923956772106No Hit
GGCACAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA200.15776603297310088No Hit
GGCACTAAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA190.14987773132444585No Hit
GGACTAGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA190.14987773132444585No Hit
AAATACCGTCAGAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGAACCT190.14987773132444585No Hit
GGCACTAGAAGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA180.1419894296757908No Hit
GGCATAGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA180.1419894296757908No Hit
AAATACCGTTCAGAGCGCCACCAGGAGCTTGCAAATTGCTGCTGGAACCT180.1419894296757908No Hit
GGCACTAGAAGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA170.13410112802713575No Hit
GGCCTAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA160.12621282637848072No Hit
AAATACCGTTCGAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGAACCT160.12621282637848072No Hit
AAATACCGTTCAGAGCTGCCACCAGGAGCTTGCAAATTGATGCTGGAACC150.11832452472982566No Hit
GGCACAGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA150.11832452472982566No Hit
AAATACCGTTAGAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGAACCT140.11043622308117064No Hit
AAATACCGTTCAGAGCTGCCCCCAGGAGCTTGCAAATTGCTGCTGGAACC140.11043622308117064No Hit
AAATACCTTCAGAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGAACCT130.10254792143251559No Hit
GGCACTAGAATGGACCCCTTCCTCATCACTACATTATTTCAATTAAATAC130.10254792143251559No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)