FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008151993

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008151993
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9816
Total Bases1.4 Mbp
Sequences flagged as poor quality0
Sequence length141-145
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTGTGTGTGTTTCTGTGGGTTTCTTTAAGGTTTGGACAGAAGGGTAAAG325333.139771801140995No Hit
ACTTAAAAACAAGGCAGTGCTAATGCCTAATGGTGCTACAGTTTCTGCCT324333.03789731051345No Hit
ACTTAAAAACAAGGCAGTGCTAATGCCAAATGGTGCTACAGTTTCTGCCT139714.231866340668297No Hit
ACTTAAAAACAAGGCAGCTCTGAACGGTATTTCCTCTAAGACCAAGTCTC3273.3312958435207825No Hit
ACTTAAAACAAGGCAGTGCTAATGCCTAATGGTGCTACAGTTTCTGCCTC1071.0900570497147515No Hit
ACTTAAAAACAAGGCAGTTCTGAACGGTATTTCCTCTAAGACCAAGTCTC840.8557457212713936No Hit
ACTTAAAACAAGGCAGTGCTAATGCCAAATGGTGCTACAGTTTCTGCCTC420.4278728606356968No Hit
ACTTAAAAACAAGGCAGTGCTGAACGGTATTTCCTCTAAGACCAAGTCTC390.3973105134474328No Hit
ACTTAAAAACAGGCAGTGCTAATGCCTAATGGTGCTACAGTTTCTGCCTC310.31581092094539526No Hit
CTTAAAAACAAGGCAGTGCTAATGCCTAATGGTGCTACAGTTTCTGCCTC250.25468622656886714No Hit
ACTTAAAAACAAGCAGTGCTAATGCCTAATGGTGCTACAGTTTCTGCCTC220.22412387938060307No Hit
ACTTAAAAACAAGGCAGTGCTATCAACGAAGAGGTCCATTCTAGTGCCCC210.2139364303178484No Hit
TGTGTGTGTGTTCTGTGGGTTTCTTTAAGGTTTGGACAGAAGGGTAAAGC190.19356153219233904No Hit
ACTAAAAACAAGGCAGTGCTAATGCCTAATGGTGCTACAGTTTCTGCCTC180.18337408312958436No Hit
ACTTAAAAACAAGGCAGTGCTATGCCCTCCCAGGTACCCCAAGCTGGCAG160.16299918500407498No Hit
TGTGTGTGTGTTTCTGTGGGTTTCTTTAAGGTTTGGACAGAAGGGTAAGG140.14262428687856563No Hit
ATTAAAAACAAGGCAGTGCTAATGCCTAATGGTGCTACAGTTTCTGCCTC140.14262428687856563No Hit
ACTTAAAAACAGGCAGTGCTAATGCCAAATGGTGCTACAGTTTCTGCCTC130.13243683781581092No Hit
ACTTAAAAACAAGGCAGTGCTGTGCCCTCCCAGGTACCCCAAGCTGGCAG130.13243683781581092No Hit
ACTTAAAAACAAGGCAGTGCTAATGCCTAATGGTGCTACAGTTTCTGCCC130.13243683781581092No Hit
ACTTAAAAAAAGGCAGTGCTAATGCCTAATGGTGCTACAGTTTCTGCCTC120.12224938875305623No Hit
CTTAAAAACAAGGCAGTGCTAATGCCAAATGGTGCTACAGTTTCTGCCTC120.12224938875305623No Hit
TGTGTGTGTTTCTGTGGGTTTCTTTAAGGTTTGGACAGAAGGGTAAAGCT110.11206193969030154No Hit
ACTAAAAACAAGGCAGTGCTAATGCCAAATGGTGCTACAGTTTCTGCCTC100.10187449062754686No Hit
ACTTAAAAACAAGGCGGTGCTAATGCCTAATGGTGCTACAGTTTCTGCCT100.10187449062754686No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)