FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008152003

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008152003
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12928
Total Bases1.8 Mbp
Sequences flagged as poor quality0
Sequence length142-145
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTGTGTGTGTTTCTGTGGGTTTCTTTAAGGTTTGGACAGAAGGGTAAAG707354.71070544554455No Hit
ACTTAAAAACAAGGCAGTGCTAATGCTAATGGTGCTACAGTTTCTGCCTC190014.696782178217822No Hit
ACTTAAAAACAAGGCAGTGCTAATGCCTAATGGTGCTACAGTTTCTGCCT171313.250309405940595No Hit
ACTTAAAAACAAGGCAGCTCTGAACGGTATTTCCTCTAAGACCAAGTCTC4613.5659034653465342No Hit
ACTTAAAAACAAGGCAGTTCTGAACGGTATTTCCTCTAAGACCAAGTCTC1260.9746287128712872No Hit
TGTGTGTGTGTTCTGTGGGTTTCTTTAAGGTTTGGACAGAAGGGTAAAGC490.37902227722772275No Hit
ACTTAAAACAAGGCAGTGCTAATGCTAATGGTGCTACAGTTTCTGCCTCT480.3712871287128713No Hit
ACTTAAAAACAAGGCAGTGCTATCAACGAAGAGGTCCATTCTAGTGCCCC450.34808168316831684No Hit
ACTTAAAACAAGGCAGTGCTAATGCCTAATGGTGCTACAGTTTCTGCCTC440.34034653465346537No Hit
ACTTAAAAACAAGGCAGTGCTGAACGGTATTTCCTCTAAGACCAAGTCTC330.25525990099009904No Hit
TGTGTGTGTGTTTCTGTGGGTTTCTTTAAGGTTTGGACAGAGGGGTAAAG320.24752475247524752No Hit
TGTGTGTGTGTTTCTGTGGGTTTCTTTAAGGTTTGGACAGAAGGGTAAGG290.22431930693069305No Hit
TGTGTGTGTGTTTCTGTGGGTTTCTTTAAGGTTTGGACAGAAGGGTAGAG230.17790841584158418No Hit
CTTAAAAACAAGGCAGTGCTAATGCTAATGGTGCTACAGTTTCTGCCTCT210.1624381188118812No Hit
TGTGTGTGTGTTTCTGTGGGTTTCTTTAGGGTTTGGACAGAAGGGTAAAG190.1469678217821782No Hit
GTGTGTGTGTTTCTGTGGGTTTCTTTAAGGTTTGGACAGAAGGGTAAAGC170.13149752475247525No Hit
TGTGTGTGTGTTTCTGTGGGCTTCTTTAAGGTTTGGACAGAAGGGTAAAG170.13149752475247525No Hit
TGTGTGTGTGTTTCTGTGGGTTTCTTTAAGGTTTGGGCAGAAGGGTAAAG170.13149752475247525No Hit
ACTTAAAAACAGGCAGTGCTAATGCCTAATGGTGCTACAGTTTCTGCCTC160.12376237623762376No Hit
TGTGTGTTGTTTCTGTGGGTTTCTTTAAGGTTTGGACAGAAGGGTAAAGC150.11602722772277227No Hit
ACTTAAAAACAGGCAGTGCTAATGCTAATGGTGCTACAGTTTCTGCCTCT150.11602722772277227No Hit
CTTAAAAACAAGGCAGTGCTAATGCCTAATGGTGCTACAGTTTCTGCCTC140.1082920792079208No Hit
TGTGTGTGTGTTTCTGTGGGTTTCTTTAAGGTTTGGACAGGAGGGTAAAG140.1082920792079208No Hit
TGTGTGTGTTTCTGTGGGTTTCTTTAAGGTTTGGACAGAAGGGTAAAGCT140.1082920792079208No Hit
TGTGTGTGTGTTTCTGTGGGTTTCCTTAAGGTTTGGACAGAAGGGTAAAG140.1082920792079208No Hit
TGTGTGTGTGTTTCTGTGGGTTTCTTTAAGGCTTGGACAGAAGGGTAAAG140.1082920792079208No Hit
TGTGTGTGTGTTTCGTGGGTTTCTTTAAGGTTTGGACAGAAGGGTAAAGC140.1082920792079208No Hit
TGTGTGTGTGTTTCTTGGGTTTCTTTAAGGTTTGGACAGAAGGGTAAAGC130.10055693069306931No Hit
TGTGTGTGTGTTTTGTGGGTTTCTTTAAGGTTTGGACAGAAGGGTAAAGC130.10055693069306931No Hit
TTGTGTGTGTTTCTGTGGGTTTCTTTAAGGTTTGGACAGAAGGGTAAAGC130.10055693069306931No Hit
TGTGTGTGTGTTTCTGTGGGTTTCTTTAAGGTTTGGACAGAAGGGTGAAG130.10055693069306931No Hit
TGTGTTGTGTTTCTGTGGGTTTCTTTAAGGTTTGGACAGAAGGGTAAAGC130.10055693069306931No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)