FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008152012

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008152012
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14883
Total Bases2.1 Mbp
Sequences flagged as poor quality0
Sequence length141-145
%GC37

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCACTAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATAC544436.57864677820332No Hit
GGCACTAGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATAC213914.372102398709938No Hit
AAATACCGTTCAGAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGAACC183512.32950346032386No Hit
AAATACCGTTCAGAGCTGCCACCAAGAGCTTGCAAATTGCTGCTGGAACC154810.401128804676476No Hit
AAATACCGTTCAGAGCTGCCTTGTTTTTAAGTTTACGCTGTAGAACCATG4893.28562789760129No Hit
AAATACCGTTCAGAGCTGCCAGCTTGGGGTACCTGGGAGGGCACAGCACT1320.8869179600886918No Hit
GGCACTAGATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA1080.7256601491634751No Hit
AAATACCGTTCAGAGCTGCCAGCTTGGGGTACCTGGGAGGGCATAGCACT1040.6987838473426057No Hit
AAATACCGTTCAGAACTGCCTTGTTTTTAAGTTTACGCTGTAGAACCATG1000.6719075455217361No Hit
GCACTAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA870.5845595646039106No Hit
GGCACTAGAATGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA610.40986360276825906No Hit
GGCACTAGATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA520.34939192367130284No Hit
GGCACTAGAATGGACCTCTTCGTTGATAGCACTGCCTTGTTTTTAAGTTT500.3359537727608681No Hit
GGCACTGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA470.31579654639521604No Hit
AAATACCGTTCAGCACTGCCTTGTTTTTAAGTTTACGCTGTAGAACCATG400.26876301820869447No Hit
GGCACTAGAAGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA390.2620439427534771No Hit
GGCACTAAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA370.2486057918430424No Hit
GGCATAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA370.2486057918430424No Hit
GGCACAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA350.23516764093260764No Hit
GGACTAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA330.22172949002217296No Hit
GCACTAGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA310.20829133911173825No Hit
AATACCGTTCAGAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGAACCT300.20157226365652087No Hit
GGCACTAGAATGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA260.17469596183565142No Hit
GGCACTAAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA220.14781966001478197No Hit
GGCACTAGAATGGACTCTTCCTCATCACTACATTATTTCAATTAAATACA210.14110058455956462No Hit
GGCACTAGAATGGCCCTCTTCCTCATCACTACATTATTTCAATTAAATAC210.14110058455956462No Hit
GGACTAGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA200.13438150910434724No Hit
GGCCTAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA190.12766243364912988No Hit
AATACCGTTCAGAGCTGCCACCAAGAGCTTGCAAATTGCTGCTGGAACCT170.11422428273869516No Hit
GGCACTAGAATGGACCCTTCCTCATCACTACATTATTTCAATTAAATACA160.10750520728347779No Hit
GGCACTGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA160.10750520728347779No Hit
GGCACTAGAATGGACCTCTTCCTCACCACTACATTATTTCAATTAAATAC160.10750520728347779No Hit
GGCACTAGAATGGACCTCTCCCTCATCACTACATTATTTCAATTAAATAC160.10750520728347779No Hit
AAATACGTTCAGAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGAACCT150.10078613182826043No Hit
AAATACCGTTCAGAGCTGCCACAGAAACACACACACATTACGCTGTAGAA150.10078613182826043No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)