FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008152024

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008152024
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14570
Total Bases2.1 Mbp
Sequences flagged as poor quality0
Sequence length142-145
%GC34

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCACTAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATAC479132.88263555250515No Hit
GGCACTAGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATAC431629.622512010981467No Hit
AAATACCGTTCAGAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGAACC188612.944406314344544No Hit
AAATACCGTTCAGAGCTGCCTTGTTTTTAAGTTTACGCTGTAGAACCATG3862.649279341111874No Hit
GGCACTAGATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA1060.727522306108442No Hit
AAATACCGTTCAGAACTGCCTTGTTTTTAAGTTTACGCTGTAGAACCATG920.6314344543582704No Hit
GGCACTAGATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA920.6314344543582704No Hit
GCACTAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA840.5765271105010296No Hit
GCACTAGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA740.5078929306794784No Hit
GGCACTAGAATGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA530.363761153054221No Hit
GGCACTAGAATGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA490.33630748112560055No Hit
GGCACTAAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA450.3088538091969801No Hit
GGCACTGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA440.30199039121482496No Hit
GGCACTGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA410.2814001372683596No Hit
GGACTAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA390.2676733013040494No Hit
AAATACCGTTCAGCACTGCCTTGTTTTTAAGTTTACGCTGTAGAACCATG380.2608098833218943No Hit
GGACTAGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA370.2539464653397392No Hit
GGCACTAGAAGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA360.24708304735758407No Hit
GGCACTAGAATGGACCTCTTCGTTGATAGCACTGCCTTGTTTTTAAGTTT350.24021962937542896No Hit
GGCACTAAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA340.23335621139327387No Hit
GGCACAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA330.22649279341111872No Hit
GGCACTAGAATGGACTCTTCCTCATCACTACATTATTTCAATTAAATACA330.22649279341111872No Hit
GGCATAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA330.22649279341111872No Hit
AATACCGTTCAGAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGAACCT300.20590253946465342No Hit
GGCATAGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA290.19903912148249828No Hit
GGCACAGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA260.17844886753603295No Hit
AAATACCGTTCAGAGCTGCCACCAAGAGCTTGCAAATTGCTGCTGGAACC250.17158544955387783No Hit
GGCCTAGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA240.16472203157172272No Hit
GGCCTAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA230.1578586135895676No Hit
GGCACTAGAATGGACTCTTCCTCATCACTAAATTATTTCAATTAAATACA230.1578586135895676No Hit
GGCACTAGAAGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA230.1578586135895676No Hit
AAATACGTTCAGAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGAACCT220.15099519560741248No Hit
GGCACTAGAATGGACCCTTCCTCATCACTAAATTATTTCAATTAAATACA190.13040494166094715No Hit
GGCACTAGAATGGCCTCTTCCTCATCACTAAATTATTTCAATTAAATACA180.12354152367879204No Hit
AAATACCGTTCAGAGCTGCCAGCTTGGGGTACCTGGGAGGGCACAGCACT180.12354152367879204No Hit
GGCACTAGAATGGACCTCTCCCTCATCACTACATTATTTCAATTAAATAC170.11667810569663693No Hit
GGCACTAGAATGGACCTCTTACTCATCACTAAATTATTTCAATTAAATAC170.11667810569663693No Hit
GGCACTAGAATGGACCCTTCCTCATCACTACATTATTTCAATTAAATACA160.1098146877144818No Hit
GGCACTAGAATGGACCTCTCCCTCATCACTAAATTATTTCAATTAAATAC160.1098146877144818No Hit
GGCACTAGAATGGCCCTCTTCCTCATCACTAAATTATTTCAATTAAATAC150.10295126973232671No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)