FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008152040

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008152040
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14539
Total Bases2.1 Mbp
Sequences flagged as poor quality0
Sequence length142-145
%GC36

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCACTAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATAC456931.425820207717177No Hit
GGCACTAGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATAC284419.561180273746476No Hit
AAATACCGTTCAGAGCTGCCACCAAGAGCTTGCAAATTGCTGCTGGAACC246916.981910722883278No Hit
AAATACCGTTCAGAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGAACC9676.651076415159227No Hit
AAATACCGTTCAGAGCTGCCTTGTTTTTAAGTTTACGCTGTAGAACCATG4743.2601967122910787No Hit
AAATACCGTTCAGAACTGCCTTGTTTTTAAGTTTACGCTGTAGAACCATG1080.7428296306486003No Hit
GGCACTAGATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA900.6190246922071669No Hit
GCACTAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA680.4677075452231928No Hit
GGCACTAGATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA610.4195611802737465No Hit
AAATACCGTTCAGAGCTGCCAGCTTGGGGTACCTGGGAGGGCACAGCACT600.4126831281381113No Hit
GGCACTAGAATGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA560.38517091959557054No Hit
AAATACCGTTCAGAGCTGCCAGCTTGGGGTACCTGGGAGGGCATAGCACT510.35078065891739457No Hit
GCACTAGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA480.33014650251048905No Hit
AAATACCGTTCAGCACTGCCTTGTTTTTAAGTTTACGCTGTAGAACCATG360.2476098768828668No Hit
GGACTAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA350.2407318247472316No Hit
GGCACTAAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA350.2407318247472316No Hit
GGCACTAGAATGGACCTCTTCGTTGATAGCACTGCCTTGTTTTTAAGTTT350.2407318247472316No Hit
AATACCGTTCAGAGCTGCCACCAAGAGCTTGCAAATTGCTGCTGGAACCT350.2407318247472316No Hit
GGCACTGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA340.2338537726115964No Hit
GGCACTAGAAGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA320.220097668340326No Hit
GGCATAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA310.21321961620469082No Hit
GGCACTAAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA300.20634156406905566No Hit
GGCACTAGAATGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA260.1788293555265149No Hit
GGCACTAGAATGGACCCTTCCTCATCACTACATTATTTCAATTAAATACA250.1719513033908797No Hit
AAATACCGTTCAGAGCTGCCATGTCATGAGTGGCCAGATGGAGAGGCCCA240.16507325125524452No Hit
GGACTAGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA230.15819519911960933No Hit
GGCACAGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA220.15131714698397414No Hit
AAATACGTTCAGAGCTGCCACCAAGAGCTTGCAAATTGCTGCTGGAACCT220.15131714698397414No Hit
AAATACCGTTCAGAGCTGCCACAGAAACACACACACATTACGCTGTAGAA210.14443909484833894No Hit
GGCACAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA200.13756104271270378No Hit
GGCACTAGAAGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA200.13756104271270378No Hit
GGCACTAGAATGGACTCTTCCTCATCACTACATTATTTCAATTAAATACA180.1238049384414334No Hit
GGCACTGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA180.1238049384414334No Hit
GGCACTAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATGC180.1238049384414334No Hit
GGCCTAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA170.1169268863057982No Hit
GGCACTAGAATGGCCCTCTTCCTCATCACTACATTATTTCAATTAAATAC160.110048834170163No Hit
AAATACCGTTCAGAGCTGCCATGTCATGAGTGGCCAGATAGAGAGGCCCA150.10317078203452783No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)