FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008152052

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008152052
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15229
Total Bases2.2 Mbp
Sequences flagged as poor quality0
Sequence length141-145
%GC37

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCACTAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATAC412927.11274541992252No Hit
AAATACCGTTCAGAGCTGCCACCAAGAGCTTGCAAATTGCTGCTGGAACC349722.962768402390175No Hit
GGCACTAGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATAC323921.268632214853238No Hit
AAATACCGTTCAGAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGAACC6324.149977017532339No Hit
AAATACCGTTCAGAGCTGCCTTGTTTTTAAGTTTACGCTGTAGAACCATG4613.027119311839254No Hit
GGCACTAGATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA1040.6829076104800053No Hit
AAATACCGTTCAGAACTGCCTTGTTTTTAAGTTTACGCTGTAGAACCATG980.6435090944907742No Hit
GCACTAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA610.4005515792238492No Hit
GCACTAGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA570.3742859018976952No Hit
GGCACTAGATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA570.3742859018976952No Hit
AAATACCGTTCAGCACTGCCTTGTTTTTAAGTTTACGCTGTAGAACCATG490.3217545472453871No Hit
GGCACTAGAATGGACCTCTTCGTTGATAGCACTGCCTTGTTTTTAAGTTT480.3151881279138486No Hit
AATACCGTTCAGAGCTGCCACCAAGAGCTTGCAAATTGCTGCTGGAACCT480.3151881279138486No Hit
AAATACCGTTCAGAGCTGCCAGCTTGGGGTACCTGGGAGGGCACAGCACT460.3020552892507715No Hit
GGCACTAGAATGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA430.282356031256156No Hit
AAATACCGTTCAGAGCTGCCAGCTTGGGGTACCTGGGAGGGCATAGCACT430.282356031256156No Hit
GGCACTAGAAGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA410.269223192593079No Hit
GGCACTAAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA360.23639109593538646No Hit
GGACTAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA330.2166918379407709No Hit
GGCACTGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA330.2166918379407709No Hit
GGCACTAGAATGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA290.19042616061461684No Hit
GGCATAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA290.19042616061461684No Hit
GGCACTAGAAGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA270.17729332195153982No Hit
GGCACAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA260.17072690262000131No Hit
GGCACTAAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA250.1641604832884628No Hit
GGCATAGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA240.1575940639569243No Hit
GGACTAGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA230.15102764462538576No Hit
GGCACTGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA210.13789480596230877No Hit
AAATACGTTCAGAGCTGCCACCAAGAGCTTGCAAATTGCTGCTGGAACCT200.13132838663077023No Hit
GGCACTAGAATGGACCCTTCCTCATCACTACATTATTTCAATTAAATACA190.12476196729923172No Hit
AAATACCGTCAGAGCTGCCACCAAGAGCTTGCAAATTGCTGCTGGAACCT190.12476196729923172No Hit
GGCACTAGAATGGACTCTTCCTCATCACTAAATTATTTCAATTAAATACA190.12476196729923172No Hit
AAATACCGTTCAGAGCTGCCACCAAGAGCTTGCAAATTGCTGCTGGACCC180.11819554796769323No Hit
AAATACCGTTCAAGCTGCCACCAAGAGCTTGCAAATTGCTGCTGGAACCT160.10506270930461618No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)