FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008152078

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008152078
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11242
Total Bases1.6 Mbp
Sequences flagged as poor quality0
Sequence length140-145
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCACTAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATAC314127.939868350827258No Hit
AAATACCGTTCAGAGCTGCCACCAAGAGCTTGCAAATTGCTGCTGGAACC194217.274506315602206No Hit
GGCACTAGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATAC157814.036648283223627No Hit
AAATACCGTTCAGAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGAACC9608.539405799679772No Hit
AAATACCGTTCAGAGCTGCCTTGTTTTTAAGTTTACGCTGTAGAACCATG7356.537982565379825No Hit
AAATACCGTTCAGAACTGCCTTGTTTTTAAGTTTACGCTGTAGAACCATG2111.8768902330546167No Hit
AAATACCGTTCAGAGCTGCCAGCTTGGGGTACCTGGGAGGGCACAGCACT1691.5032912293186267No Hit
AAATACCGTTCAGAGCTGCCAGCTTGGGGTACCTGGGAGGGCATAGCACT860.764988436221313No Hit
AAATACCGTTCAGCACTGCCTTGTTTTTAAGTTTACGCTGTAGAACCATG860.764988436221313No Hit
GGCACTAGATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA570.5070272193559865No Hit
GCACTAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA470.41807507560932217No Hit
GGCACTAGATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA310.2757516456146593No Hit
GGCACTAGAATGGACCTCTTCGTTGATAGCACTGCCTTGTTTTTAAGTTT280.24906600249066002No Hit
GCACTAGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA270.24017078811599363No Hit
AATACCGTTCAGAGCTGCCACCAAGAGCTTGCAAATTGCTGCTGGAACCT260.23127557374132715No Hit
GGCACTAGAAGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA250.22238035936666076No Hit
GGCATAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA250.22238035936666076No Hit
GGCACTAGAATGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA240.2134851449919943No Hit
GGCACTAAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA230.2045899306173279No Hit
GGCACTGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA230.2045899306173279No Hit
GGACTAGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA200.1779042874933286No Hit
GGCACTAAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA190.16900907311866217No Hit
GGACTAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA170.1512186443693293No Hit
AAATACCGTTCAGAGCTGCCACAGAAACACACACACATTACGCTGTAGAA170.1512186443693293No Hit
GGCACAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA160.14232342999466288No Hit
GGCCTAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA150.13342821561999643No Hit
AAATACCGTCAGAGCTGCCACCAAGAGCTTGCAAATTGCTGCTGGAACCT150.13342821561999643No Hit
AAATACCGTTCAGAGCTGCCACCAAGAGCTTGCAAATTGCTGCTGGACCC150.13342821561999643No Hit
GGCACTAGAATGGACCTCTCCCTCATCACTACATTATTTCAATTAAATAC150.13342821561999643No Hit
GGCACTAGAATGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA140.12453300124533001No Hit
GGCACTAGAATGGACTCTTCCTCATCACTACATTATTTCAATTAAATACA140.12453300124533001No Hit
AATACCGTTCAGAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGAACCT130.11563778687066358No Hit
GGCACTAGAATGGCCCTCTTCCTCATCACTACATTATTTCAATTAAATAC120.10674257249599715No Hit
AAATACCGTTCAGAGCTGCCTTGTTTTAAGTTTACGCTGTAGAACCATGT120.10674257249599715No Hit
GGCACAGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA120.10674257249599715No Hit
AAATACGTTCAGAGCTGCCACCAAGAGCTTGCAAATTGCTGCTGGAACCT120.10674257249599715No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)