FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008152080

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008152080
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11127
Total Bases1.6 Mbp
Sequences flagged as poor quality0
Sequence length143-145
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCACTAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATAC359532.30879841826189No Hit
AAATACCGTTCAGAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGAACC254222.845331176417723No Hit
AAATACCGTTCAGAGCTGCCACCAAGAGCTTGCAAATTGCTGCTGGAACC10079.050058416464456No Hit
AAATACCGTTCAGAGCTGCCTTGTTTTTAAGTTTACGCTGTAGAACCATG7226.488721128785836No Hit
GGCACTAGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATAC4624.15206255055271No Hit
AAATACCGTTCAGAACTGCCTTGTTTTTAAGTTTACGCTGTAGAACCATG2252.0221083850094366No Hit
AAATACCGTTCAGAGCTGCCAGCTTGGGGTACCTGGGAGGGCACAGCACT1701.527815224229352No Hit
GGCACTAGATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA760.6830232767142985No Hit
GCACTAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA760.6830232767142985No Hit
AAATACCGTTCAGAGCTGCCAGCTTGGGGTACCTGGGAGGGCATAGCACT660.5931517929361013No Hit
AAATACCGTTCAGCACTGCCTTGTTTTTAAGTTTACGCTGTAGAACCATG640.575177496180462No Hit
AATACCGTTCAGAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGAACCT500.4493574188909859No Hit
GGACTAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA430.38644738024624786No Hit
GGCACTAGAATGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA380.34151163835714926No Hit
GGCACTGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA360.3235373416015098No Hit
GGCACTAGAAGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA340.3055630448458704No Hit
GGCACAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA330.29657589646805066No Hit
GGCACTAAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA330.29657589646805066No Hit
GGCATAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA270.24265300620113237No Hit
AATACCGTTCAGAGCTGCCACCAAGAGCTTGCAAATTGCTGCTGGAACCT230.2067044126898535No Hit
GGCACTAGAATGGACCCTTCCTCATCACTACATTATTTCAATTAAATACA200.17974296755639435No Hit
AAATACCGTTCAGAGCTGCCTTGTTTTAAGTTTACGCTGTAGAACCATGT200.17974296755639435No Hit
AAATACCGTCAGAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGAACCT200.17974296755639435No Hit
AAATACCGTTCAGAGCTGCCACAGAAACACACACACATTACGCTGTAGAA180.1617686708007549No Hit
AAATACGTTCAGAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGAACCT170.1527815224229352No Hit
GGCACTAGAATGGACTCTTCCTCATCACTACATTATTTCAATTAAATACA170.1527815224229352No Hit
GGCCTAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA160.1437943740451155No Hit
GGCACTAGAATGGCCCTCTTCCTCATCACTACATTATTTCAATTAAATAC150.13480722566729578No Hit
AAATACCGTTCAGAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGACCC150.13480722566729578No Hit
GGCACTAGAATGGACCTCTTCGTTGATAGCACTGCCTTGTTTTTAAGTTT150.13480722566729578No Hit
GGCACTAGAATGGCCTCTTCCTCATCACTACATTATTTCAATTAAATACA140.12582007728947606No Hit
AAATACCGTTCAGAGCTGCCATTGCCCCACCCACTTCTCTGGTAGAGAAA130.11683292891165632No Hit
AAATACCGTTCAGAGCGCCACCAGGAGCTTGCAAATTGCTGCTGGAACCT130.11683292891165632No Hit
GGCACTAGAATGGACCTCTCCCTCATCACTACATTATTTCAATTAAATAC130.11683292891165632No Hit
AAATACCGTTCAAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGAACCT120.10784578053383662No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)