FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008152104

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008152104
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13884
Total Bases2 Mbp
Sequences flagged as poor quality0
Sequence length141-145
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCACTAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATAC585442.16364160184385No Hit
AAATACCGTTCAGAGCTGCCACCAAGAGCTTGCAAATTGCTGCTGGAACC272719.64131374243734No Hit
AAATACCGTTCAGAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGAACC8305.978104292711034No Hit
GGCACTAGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATAC7505.4019014693172No Hit
AAATACCGTTCAGAGCTGCCTTGTTTTTAAGTTTACGCTGTAGAACCATG5253.78133102852204No Hit
GGCACTAGATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA1330.9579371938922501No Hit
AAATACCGTTCAGAACTGCCTTGTTTTTAAGTTTACGCTGTAGAACCATG1230.8859118409680208No Hit
AAATACCGTTCAGAGCTGCCAGCTTGGGGTACCTGGGAGGGCACAGCACT1130.8138864880437915No Hit
GCACTAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA1000.7202535292422932No Hit
AAATACCGTTCAGAGCTGCCAGCTTGGGGTACCTGGGAGGGCATAGCACT610.4393546528377989No Hit
GGCACTAAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA600.432152117545376No Hit
AAATACCGTTCAGCACTGCCTTGTTTTTAAGTTTACGCTGTAGAACCATG530.3817343704984154No Hit
GGCACTAGAATGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA480.34572169403630076No Hit
GGCACAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA440.31691155286660905No Hit
GGACTAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA410.29530394698934026No Hit
GGCACTAGAATGGACCTCTTCGTTGATAGCACTGCCTTGTTTTTAAGTTT410.29530394698934026No Hit
GGCACTGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA380.2736963411120715No Hit
GGCATAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA350.25208873523480263No Hit
GGCCTAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA310.2232785940651109No Hit
AATACCGTTCAGAGCTGCCACCAAGAGCTTGCAAATTGCTGCTGGAACCT310.2232785940651109No Hit
GGCACTAGAAGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA300.216076058772688No Hit
GGCACTAGAATGGACCCTTCCTCATCACTACATTATTTCAATTAAATACA290.20887352348026503No Hit
AAATACCGTCAGAGCTGCCACCAAGAGCTTGCAAATTGCTGCTGGAACCT260.18726591760299627No Hit
GGCACTAGAATGGACTCTTCCTCATCACTACATTATTTCAATTAAATACA250.1800633823105733No Hit
AAATACCGTTCAGAGCTGCCACCAAGAGCTTGCAAATTGCTGCTGGACCC240.17286084701815038No Hit
AAATACGTTCAGAGCTGCCACCAAGAGCTTGCAAATTGCTGCTGGAACCT240.17286084701815038No Hit
GGCACTAGAATGGCCCTCTTCCTCATCACTACATTATTTCAATTAAATAC230.16565831172572745No Hit
GGCACTAGATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA210.1512532411408816No Hit
GGCACTAGAATGGACCTCTTCCCCATCACTACATTATTTCAATTAAATAC170.12244309997118986No Hit
GGCACTAGAATGGACCTCTTCCTCATCACTACGTTATTTCAATTAAATAC160.11524056467876692No Hit
GGCACTAGAATGGACCTCTCCCTCATCACTACATTATTTCAATTAAATAC160.11524056467876692No Hit
AAATACCGTTCAGAGCTGCCACAGAAACACACACACATTACGCTGTAGAA150.108038029386344No Hit
AAATACCGTTAGAGCTGCCACCAAGAGCTTGCAAATTGCTGCTGGAACCT140.10083549409392106No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)