FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008152106

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008152106
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10772
Total Bases1.5 Mbp
Sequences flagged as poor quality0
Sequence length142-145
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCACTAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATAC403137.421091719272184No Hit
AAATACCGTTCAGAGCTGCCACCAAGAGCTTGCAAATTGCTGCTGGAACC188917.53620497586335No Hit
AAATACCGTTCAGAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGAACC175116.255105829929448No Hit
AAATACCGTTCAGAGCTGCCTTGTTTTTAAGTTTACGCTGTAGAACCATG5535.133679910880059No Hit
AAATACCGTTCAGAACTGCCTTGTTTTTAAGTTTACGCTGTAGAACCATG1331.2346825102116599No Hit
GGCACTAGATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA990.9190493873004085No Hit
GCACTAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA810.7519494987003341No Hit
AAATACCGTTCAGCACTGCCTTGTTTTTAAGTTTACGCTGTAGAACCATG530.4920163386557742No Hit
AAATACCGTTCAGAGCTGCCAGCTTGGGGTACCTGGGAGGGCACAGCACT520.4827330115113257No Hit
GGCACTAGAATGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA480.44559970293353135No Hit
GGCACTAAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA430.39918306721128854No Hit
AAATACCGTTCAGAGCTGCCAGCTTGGGGTACCTGGGAGGGCATAGCACT370.3434831043445971No Hit
GGCACTAGAATGGACCTCTTCGTTGATAGCACTGCCTTGTTTTTAAGTTT350.32491645005569997No Hit
GGCACAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA330.3063497957668028No Hit
AATACCGTTCAGAGCTGCCACCAAGAGCTTGCAAATTGCTGCTGGAACCT320.29706646862235425No Hit
GGACTAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA310.2877831414779057No Hit
GGCACTGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA310.2877831414779057No Hit
GGCATAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA270.2506498329001114No Hit
AATACCGTTCAGAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGAACCT250.23208317861121425No Hit
AAATACGTTCAGAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGAACCT220.20423319717786853No Hit
GGCACTAGAATGGCCCTCTTCCTCATCACTACATTATTTCAATTAAATAC200.18566654288897141No Hit
GGCACTAGAATGGACCTCTCCCTCATCACTACATTATTTCAATTAAATAC190.17638321574452284No Hit
GGCACTAGAATGGACCCTTCCTCATCACTACATTATTTCAATTAAATACA180.16709988860007427No Hit
GGCCTAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA170.1578165614556257No Hit
GGCACTAGAAGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA170.1578165614556257No Hit
GGCACTAGAATGGACCTCTTCCCCATCACTACATTATTTCAATTAAATAC170.1578165614556257No Hit
AAATACCGTTCAGAGCTGCCTTGTTTTAAGTTTACGCTGTAGAACCATGT160.14853323431117713No Hit
AAATACCGTTCAGAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGACCC160.14853323431117713No Hit
AAATACGTTCAGAGCTGCCACCAAGAGCTTGCAAATTGCTGCTGGAACCT160.14853323431117713No Hit
AAATACCGTTCAAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGAACCT160.14853323431117713No Hit
AAATACCGTTCAGAGCTGCCCCCAAGAGCTTGCAAATTGCTGCTGGAACC130.12068325287783142No Hit
AAATACCGTCAGAGCTGCCACCAAGAGCTTGCAAATTGCTGCTGGAACCT120.11139992573338284No Hit
AAATACCGTTCAGAGCTGCCACAGAAACACACACACATTACGCTGTAGAA120.11139992573338284No Hit
GGCACTAGAATGGCCTCTTCCTCATCACTACATTATTTCAATTAAATACA120.11139992573338284No Hit
AAATACCGTTCAGAGCTGCCACCAAGAGCTTGCAAATTGATGCTGGAACC110.10211659858893427No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)