FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008152108

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008152108
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10152
Total Bases1.4 Mbp
Sequences flagged as poor quality0
Sequence length141-145
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCACTAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATAC388138.22892040977147No Hit
AAATACCGTTCAGAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGAACC225322.192671394799053No Hit
AAATACCGTTCAGAGCTGCCACCAAGAGCTTGCAAATTGCTGCTGGAACC9189.042553191489363No Hit
AAATACCGTTCAGAGCTGCCTTGTTTTTAAGTTTACGCTGTAGAACCATG5935.841213553979512No Hit
AAATACCGTTCAGAACTGCCTTGTTTTTAAGTTTACGCTGTAGAACCATG1731.7040977147360126No Hit
AAATACCGTTCAGAGCTGCCAGCTTGGGGTACCTGGGAGGGCACAGCACT1261.2411347517730498No Hit
AAATACCGTTCAGCACTGCCTTGTTTTTAAGTTTACGCTGTAGAACCATG680.6698187549251379No Hit
GGCACTAGATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA650.64026792750197No Hit
GCACTAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA620.6107171000788022No Hit
AAATACCGTTCAGAGCTGCCAGCTTGGGGTACCTGGGAGGGCATAGCACT570.5614657210401891No Hit
GGCACTAGAATGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA390.38416075650118203No Hit
GGCACTAGAATGGACCTCTTCGTTGATAGCACTGCCTTGTTTTTAAGTTT390.38416075650118203No Hit
GGCACTAAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA330.3250591016548463No Hit
GGCACTAGAAGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA330.3250591016548463No Hit
AATACCGTTCAGAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGAACCT300.29550827423167847No Hit
GGCATAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA300.29550827423167847No Hit
GGACTAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA260.256107171000788No Hit
GGCACTGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA260.256107171000788No Hit
GGCCTAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA210.20685579196217493No Hit
GGCACTAGAATGGACTCTTCCTCATCACTACATTATTTCAATTAAATACA210.20685579196217493No Hit
GGCACTAGAATGGACCCTTCCTCATCACTACATTATTTCAATTAAATACA210.20685579196217493No Hit
AAATACGTTCAGAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGAACCT200.1970055161544523No Hit
GGCACTAGAATGGCCCTCTTCCTCATCACTACATTATTTCAATTAAATAC200.1970055161544523No Hit
GGCACAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA180.1773049645390071No Hit
AATACCGTTCAGAGCTGCCACCAAGAGCTTGCAAATTGCTGCTGGAACCT170.16745468873128447No Hit
AAATACCGTTCGAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGAACCT150.14775413711583923No Hit
GGCACTAGAATGGACCTCTTCCCCATCACTACATTATTTCAATTAAATAC120.1182033096926714No Hit
AAATACCGTTCAGAGCTGCCCCCAAGAGCTTGCAAATTGCTGCTGGAACC120.1182033096926714No Hit
AAATACCGTTCAGAGCTGCCTTGTTTTAAGTTTACGCTGTAGAACCATGT120.1182033096926714No Hit
AAATACCGTTCAGAGCTGCCACAGAAACACACACACATTACGCTGTAGAA120.1182033096926714No Hit
AAATACCGTTCAAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGAACCT120.1182033096926714No Hit
GGCACTAGAATGGACCTCTTCGTCATCACTACATTATTTCAATTAAATAC110.10835303388494878No Hit
AAATACCGTTCAGAGCTGCCCCCAGGAGCTTGCAAATTGCTGCTGGAACC110.10835303388494878No Hit
AAATACCGTCAGAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGAACCT110.10835303388494878No Hit
GGCACTAGAATGGACCTCTTCCTCACCACTACATTATTTCAATTAAATAC110.10835303388494878No Hit
GGCACTAGAATGGCCTCTTCCTCATCACTACATTATTTCAATTAAATACA110.10835303388494878No Hit
GGCACTAGAATGGACCTCTTCTCATCACTACATTATTTCAATTAAATACA110.10835303388494878No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)