FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008152130

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008152130
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12131
Total Bases1.7 Mbp
Sequences flagged as poor quality0
Sequence length142-145
%GC33

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCACTAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATAC593248.899513642733496No Hit
GGCACTAGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATAC258021.267826230319017No Hit
AAATACCGTTCAGAGCTGCCACCAAGAGCTTGCAAATTGCTGCTGGAACC6024.962492787074437No Hit
AAATACCGTTCAGAGCTGCCTTGTTTTTAAGTTTACGCTGTAGAACCATG2492.052592531530789No Hit
GGCACTAGATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA1341.1046080290165692No Hit
GCACTAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA1100.9067677850136016No Hit
AAATACCGTTCAGAACTGCCTTGTTTTTAAGTTTACGCTGTAGAACCATG760.6264941060093975No Hit
GGCACTAGATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA750.6182507625092737No Hit
GGCACTAGAATGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA660.5440606710081609No Hit
GGACTAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA530.4368972055065534No Hit
GGCACTGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA530.4368972055065534No Hit
AAATACCGTTCAGAGCTGCCAGCTTGGGGTACCTGGGAGGGCACAGCACT490.4039238315060588No Hit
GGCACTAAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA460.37919380100568795No Hit
GGCACAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA430.35446377050531697No Hit
GCACTAGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA420.3462204270051933No Hit
GGCATAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA410.33797708350506966No Hit
AAATACCGTTCAGAGCTGCCAGCTTGGGGTACCTGGGAGGGCATAGCACT380.31324705300469874No Hit
GGCACTAGAAGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA370.30500370950457506No Hit
GGCACTAAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA350.28851702250432776No Hit
GGCACTAGAATGGACTCTTCCTCATCACTACATTATTTCAATTAAATACA320.26378699200395683No Hit
GGCACTAGAATGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA310.25554364850383315No Hit
AAATACCGTTCAGCACTGCCTTGTTTTTAAGTTTACGCTGTAGAACCATG310.25554364850383315No Hit
GGCCTAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA300.24730030500370953No Hit
AAATACCGTTCAGAGCTGCCATGTCATGAGTGGCCAGATGGAGAGGCCCA260.2143269310032149No Hit
GGCATAGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA260.2143269310032149No Hit
GGCACTAGAATGGACCTCTCCCTCATCACTACATTATTTCAATTAAATAC230.18959690050284397No Hit
GGCACTGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA220.1813535570027203No Hit
GGACTAGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA210.17311021350259664No Hit
GGCACTAGAATGGACTCTTCCTCATCACTAAATTATTTCAATTAAATACA200.16486687000247302No Hit
GGCACTAGAATGGACCTCTTCGTTGATAGCACTGCCTTGTTTTTAAGTTT200.16486687000247302No Hit
GGCACTAGAATGGACCCTTCCTCATCACTACATTATTTCAATTAAATACA180.1483801830022257No Hit
GGCACTAGAATGGCCCTCTTCCTCATCACTACATTATTTCAATTAAATAC180.1483801830022257No Hit
GGCACTAGAATGGACCTCTTCCCCATCACTACATTATTTCAATTAAATAC160.13189349600197842No Hit
GGCACTAGAAGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA160.13189349600197842No Hit
GGCCTAGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA150.12365015250185477No Hit
GGCACAGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA150.12365015250185477No Hit
GGCACTAGAATGGACCTCTTCCTCATCACCACATTATTTCAATTAAATAC140.1154068090017311No Hit
GGCACTAGAATGGACCTCTTCCTCATCACTACGTTATTTCAATTAAATAC140.1154068090017311No Hit
GGCACTAGAATGGACCTCTTCCCCATCACTAAATTATTTCAATTAAATAC140.1154068090017311No Hit
GGCACTAGAATGGACCTCTTCCTCATCACTACATTATTTAAATTAAATAC140.1154068090017311No Hit
GGCACTAGAATGGACCCCTTCCTCATCACTACATTATTTCAATTAAATAC130.10716346550160745No Hit
GGCACTAGAATGGACCCTTCCTCATCACTAAATTATTTCAATTAAATACA130.10716346550160745No Hit
GGCACTAGAATGGCCTCTTCCTCATCACTACATTATTTCAATTAAATACA130.10716346550160745No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)