FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008152138

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008152138
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13251
Total Bases1.9 Mbp
Sequences flagged as poor quality0
Sequence length142-145
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCACTAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATAC346426.14142328880839No Hit
GGCACTAGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATAC297422.44358916308203No Hit
AAATACCGTTCAGAGCTGCCACCAAGAGCTTGCAAATTGCTGCTGGAACC221916.745905969360802No Hit
AAATACCGTTCAGAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGAACC12029.07101350841446No Hit
AAATACCGTTCAGAGCTGCCTTGTTTTTAAGTTTACGCTGTAGAACCATG5003.7733001282922043No Hit
AAATACCGTTCAGAACTGCCTTGTTTTTAAGTTTACGCTGTAGAACCATG1591.199909440796921No Hit
AAATACCGTTCAGAGCTGCCAGCTTGGGGTACCTGGGAGGGCACAGCACT690.5207154177043243No Hit
GCACTAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA630.4754358161648178No Hit
GGCACTAGATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA610.46034261565164897No Hit
GGCACTAGATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA580.4377028148818957No Hit
GCACTAGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA500.37733001282922046No Hit
GGCACTAGAATGGACCTCTTCGTTGATAGCACTGCCTTGTTTTTAAGTTT490.369783412572636No Hit
AAATACCGTTCAGCACTGCCTTGTTTTTAAGTTTACGCTGTAGAACCATG380.2867708097502075No Hit
AAATACCGTTCAGAGCTGCCAGCTTGGGGTACCTGGGAGGGCATAGCACT370.2792242094936231No Hit
GGCACTGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA350.26413100898045433No Hit
GGCACTAGAATGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA340.2565844087238699No Hit
GGACTAGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA290.21885140744094786No Hit
GGCACAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA280.21130480718436345No Hit
GGCATAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA270.20375820692777905No Hit
GGCACTAAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA240.1811184061580258No Hit
GGCACTAAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA240.1811184061580258No Hit
AATACCGTTCAGAGCTGCCACCAAGAGCTTGCAAATTGCTGCTGGAACCT240.1811184061580258No Hit
GGCACTAGAAGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA230.17357180590144142No Hit
GGCACAGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA230.17357180590144142No Hit
GGACTAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA210.15847860538827258No Hit
GGCATAGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA210.15847860538827258No Hit
GGCACTAGAATGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA200.15093200513168817No Hit
GGCACTGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA190.14338540487510376No Hit
GGCACTAGAATGGACTCTTCCTCATCACTAAATTATTTCAATTAAATACA190.14338540487510376No Hit
GGCACTAGAATGGACTCTTCCTCATCACTACATTATTTCAATTAAATACA150.11319900384876612No Hit
GGCACTAGAATGGCCCTCTTCCTCATCACTACATTATTTCAATTAAATAC150.11319900384876612No Hit
AATACCGTTCAGAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGAACCT140.10565240359218173No Hit
GGCACTAGAAGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA140.10565240359218173No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)