FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008152156

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008152156
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14478
Total Bases2 Mbp
Sequences flagged as poor quality0
Sequence length143-145
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCACTAGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATAC445630.777731730902058No Hit
AAATACCGTTCAGAGCTGCCACCAAGAGCTTGCAAATTGCTGCTGGAACC269318.60063544688493No Hit
GGCACTAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATAC258517.854676060229313No Hit
AAATACCGTTCAGAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGAACC10257.079707141870424No Hit
AAATACCGTTCAGAGCTGCCTTGTTTTTAAGTTTACGCTGTAGAACCATG3622.500345351567896No Hit
GGCACTAGAATGGACCTCTTAGCACTGCCTTGTTTTTAAGTTTACGCTGT1200.8288437629506838No Hit
GGCACTAGATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA1100.7597734493714602No Hit
GCACTAGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA1030.7114242298660036No Hit
AAATACCGTTCAGAACTGCCTTGTTTTTAAGTTTACGCTGTAGAACCATG840.5801906340654787No Hit
GGCACTAGAATGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA710.49039922641248795No Hit
GGCACTAGATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA680.4696781323387208No Hit
GCACTAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA650.4489570382649537No Hit
GGACTAGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA540.3729796933278077No Hit
GGCACTAAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA450.31081641110650643No Hit
AAATACCGTTCAGAGCTGCCAGCTTGGGGTACCTGGGAGGGCACAGCACT400.2762812543168946No Hit
AATACCGTTCAGAGCTGCCACCAAGAGCTTGCAAATTGCTGCTGGAACCT400.2762812543168946No Hit
GGCACTAGAATGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA380.26246719160104987No Hit
GGCACTGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA380.26246719160104987No Hit
AAATACCGTTCAGCACTGCCTTGTTTTTAAGTTTACGCTGTAGAACCATG350.24174609752728277No Hit
AAATACCGTTCAGAGCTGCCACCAGGAGCTTACAAATTGCTGCTGGAACC350.24174609752728277No Hit
GGCACTAGAAGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA340.2348390661693604No Hit
AAATACCGTTCAGAGCTGCCAGCTTGGGGTACCTGGGAGGGCATAGCACT310.2141179720955933No Hit
AAATACGTTCAGAGCTGCCACCAAGAGCTTGCAAATTGCTGCTGGAACCT280.19339687802182623No Hit
GGACTAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA270.18648984666390386No Hit
GGCACTAGAATGGACTCTTCCTCATCACTAAATTATTTCAATTAAATACA270.18648984666390386No Hit
GGCCTAGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA240.16576875259013676No Hit
GGCACAGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA240.16576875259013676No Hit
GGCACTAAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA230.1588617212322144No Hit
GGCACTGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA230.1588617212322144No Hit
GGCATAGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA220.15195468987429203No Hit
AATACCGTTCAGAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGAACCT210.14504765851636967No Hit
GGCACTAGAAGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA190.13123359580052493No Hit
GGCACTAGAATGGACCTCTTCGCACTGCCTTGTTTTTAAGTTTACGCTGT180.12432656444260257No Hit
AAATACCGTCAGAGCTGCCACCAAGAGCTTGCAAATTGCTGCTGGAACCT180.12432656444260257No Hit
GGCACTAGAATGGCCCTCTTCCTCATCACTAAATTATTTCAATTAAATAC180.12432656444260257No Hit
GGCACAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA170.1174195330846802No Hit
GGCCTAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA160.11051250172675785No Hit
AAATACCGTTCAAGCTGCCACCAAGAGCTTGCAAATTGCTGCTGGAACCT150.10360547036883548No Hit
GGCACTAGAATGGACTCTTCCTCATCACTACATTATTTCAATTAAATACA150.10360547036883548No Hit
GGCACTAGAATGGACCTCTCCCTCATCACTAAATTATTTCAATTAAATAC150.10360547036883548No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)