FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008152166

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008152166
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14555
Total Bases2.1 Mbp
Sequences flagged as poor quality0
Sequence length142-145
%GC35

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCACTAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATAC535736.80522157334249No Hit
GGCACTAGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATAC332622.851253864651323No Hit
AAATACCGTTCAGAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGAACC224915.45173479903813No Hit
AAATACCGTTCAGAGCTGCCTTGTTTTTAAGTTTACGCTGTAGAACCATG4192.8787358296118173No Hit
GGCACTAGATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA1300.8931638612160768No Hit
AAATACCGTTCAGAACTGCCTTGTTTTTAAGTTTACGCTGTAGAACCATG1110.7626245276537272No Hit
GCACTAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA880.6046032291308828No Hit
AAATACCGTTCAGAGCTGCCACAGAAACACACACACATTACGCTGTAGAA690.4740638955685331No Hit
GGCACTAGATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA580.39848849192717284No Hit
GCACTAGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA520.3572655444864308No Hit
GGCACTAGAATGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA510.3503950532463071No Hit
GGACTAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA480.3297835795259361No Hit
GGCACTAAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA450.3091721058055651No Hit
AAATACCGTTCAGCACTGCCTTGTTTTTAAGTTTACGCTGTAGAACCATG410.28169014084507044No Hit
GGCACTGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA400.27481964960494676No Hit
GGCACTAGAATGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA360.24733768464445205No Hit
AATACCGTTCAGAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGAACCT350.24046719340432843No Hit
GGCACTAGAATGGACCTCTTCGTTGATAGCACTGCCTTGTTTTTAAGTTT340.23359670216420475No Hit
GGCACTAGAAGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA310.21298522844383372No Hit
GGCACAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA270.18550326348333906No Hit
GGCATAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA270.18550326348333906No Hit
GGCACTAGAAGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA260.1786327722432154No Hit
GGCACTAAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA250.1717622810030917No Hit
GGCACTAGAATGGACCCTTCCTCATCACTACATTATTTCAATTAAATACA250.1717622810030917No Hit
GGCACTGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA250.1717622810030917No Hit
AAATACGTTCAGAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGAACCT240.16489178976296806No Hit
GGCCTAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA230.15802129852284438No Hit
GGCACTAGAATGGCCCTCTTCCTCATCACTACATTATTTCAATTAAATAC230.15802129852284438No Hit
GGCACTAGAATGGACTCTTCCTCATCACTACATTATTTCAATTAAATACA220.1511508072827207No Hit
GGCCTAGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA210.14428031604259703No Hit
GGCACTAGAATGGACTCTTCCTCATCACTAAATTATTTCAATTAAATACA200.13740982480247338No Hit
AAATACCGTTCAGAGCTGCCCCCAGGAGCTTGCAAATTGCTGCTGGAACC190.1305393335623497No Hit
GGACTAGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA180.12366884232222602No Hit
GGCACTAGAATGGACCCTTCCTCATCACTAAATTATTTCAATTAAATACA170.11679835108210238No Hit
GGCACTAGAATGGCCCTCTTCCTCATCACTAAATTATTTCAATTAAATAC150.10305736860185502No Hit
GGCACTAGAATGGACCTCTCCCTCATCACTACATTATTTCAATTAAATAC150.10305736860185502No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)