FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008152169

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008152169
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10237
Total Bases1.4 Mbp
Sequences flagged as poor quality0
Sequence length141-145
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTGTGTGTGTTTCTGTGGGTTTCTTTAAGGTTTGGACAGAAGGGTAAAG466745.58952818208459No Hit
ACTTAAAAACAAGGCAGTGCTAATGCTAATGGTGCTACAGTTTCTGCCTC219521.441828660740452No Hit
ACTTAAAAACAAGGCAGTGCTAATGCCTAATGGTGCTACAGTTTCTGCCT115811.311907785484028No Hit
ACTTAAAAACAAGGCAGCTCTGAACGGTATTTCCTCTAAGACCAAGTCTC4314.210217837257009No Hit
ACTTAAAAACAAGGCAGTTCTGAACGGTATTTCCTCTAAGACCAAGTCTC1161.13314447592068No Hit
ACTTAAAAACAAGGCAGTGCTGTGCCCTCCCAGGTACCCCAAGCTGGCAG910.8889323043860506No Hit
ACTTAAAAACAAGGCAGTGCTATGCCCTCCCAGGTACCCCAAGCTGGCAG870.84985835694051No Hit
ACTTAAAACAAGGCAGTGCTAATGCTAATGGTGCTACAGTTTCTGCCTCT650.6349516459900362No Hit
ACTTAAAAACAAGGCAGTGCTGAACGGTATTTCCTCTAAGACCAAGTCTC470.4591188824851031No Hit
ACTTAAAAACAAGGCAGTGCTATCAACGAAGAGGTCCATTCTAGTGCCCC390.38097098759402165No Hit
ACTTAAAACAAGGCAGTGCTAATGCCTAATGGTGCTACAGTTTCTGCCTC350.341897040148481No Hit
TGTGTGTGTGTTCTGTGGGTTTCTTTAAGGTTTGGACAGAAGGGTAAAGC250.2442121715346293No Hit
TGTGTGTGTGTTTCTGTGGGTCAGGAATCCAGGCGTGACTCAGCTGGGTT230.22467519781185896No Hit
CTTAAAAACAAGGCAGTGCTAATGCTAATGGTGCTACAGTTTCTGCCTCT220.21490671095047378No Hit
ACTTAAAAACAGGCAGTGCTAATGCTAATGGTGCTACAGTTTCTGCCTCT170.16606427664354792No Hit
TGTGTGTGTGTTTCTGTGGGTTTCTTTAAGGTTTGGACAGAAGGGTAAGG160.15629578978216274No Hit
TGTGTGTGTGTTTCTGTGGGTTTCTTTAAGGTTTGGACAGAGGGGTAAAG160.15629578978216274No Hit
TGTGTGTGTGTTTCTGTGGGTTTCTTTAAGGTTTGGACAGGAGGGTAAAG150.14652730292077756No Hit
TGTGTGTGTGTTTCTGTGGCAGCTCTGAACGGTATTTCCTCTAAGACCAA150.14652730292077756No Hit
TGTGTGTGTGTTTCTGTGGGTTCCTTTAAGGTTTGGACAGAAGGGTAAAG140.1367588160593924No Hit
TGTGTGTGTGTTTCTGTGGGTTTCTTTAAGGTTTGGGCAGAAGGGTAAAG130.12699032919800723No Hit
ACTTAAAAACAAGCAGTGCTAATGCTAATGGTGCTACAGTTTCTGCCTCT120.11722184233662206No Hit
ACTAAAAACAAGGCAGTGCTAATGCTAATGGTGCTACAGTTTCTGCCTCT110.10745335547523689No Hit
TGTGTGTGTGTTTCTGTGGGTTTCTTTAAGGTTTGGACGGAAGGGTAAAG110.10745335547523689No Hit
TTGTGTGTGTTTCTGTGGGTTTCTTTAAGGTTTGGACAGAAGGGTAAAGC110.10745335547523689No Hit
TGTGTGTGTGTTTCTGTGGGTTTCTTTAAGGTTTGGACAGAAGGGTAGAG110.10745335547523689No Hit
TGTGTGTGTGTTTCTGTGGGTTTCCTTAAGGTTTGGACAGAAGGGTAAAG110.10745335547523689No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)