FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008152170

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008152170
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10237
Total Bases1.4 Mbp
Sequences flagged as poor quality0
Sequence length141-145
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCACTAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATAC414240.46107257985738No Hit
AAATACCGTTCAGAGCTGCCACCAAGAGCTTGCAAATTGCTGCTGGAACC200019.53697372277034No Hit
AAATACCGTTCAGAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGAACC109710.716030086939533No Hit
AAATACCGTTCAGAGCTGCCTTGTTTTTAAGTTTACGCTGTAGAACCATG4204.102764481781771No Hit
AAATACCGTTCAGAACTGCCTTGTTTTTAAGTTTACGCTGTAGAACCATG1151.1233759890592947No Hit
AAATACCGTTCAGAGCTGCCAGCTTGGGGTACCTGGGAGGGCATAGCACT950.9280062518315914No Hit
AAATACCGTTCAGAGCTGCCAGCTTGGGGTACCTGGGAGGGCACAGCACT900.8791638175246654No Hit
GGCACTAGATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA780.7619419751880433No Hit
GCACTAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA710.6935625671583472No Hit
AAATACCGTTCAGCACTGCCTTGTTTTTAAGTTTACGCTGTAGAACCATG490.47865585620787343No Hit
GGCACTAGAATGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA460.4493503956237179No Hit
AATACCGTTCAGAGCTGCCACCAAGAGCTTGCAAATTGCTGCTGGAACCT410.400507961316792No Hit
GGCACTAGAATGGACCTCTTCGTTGATAGCACTGCCTTGTTTTTAAGTTT380.3712025007326365No Hit
GGCACTGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA350.341897040148481No Hit
GGACTAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA310.30282309270294033No Hit
GGCACTAAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA310.30282309270294033No Hit
AAATACCGTTCAGAGCTGCCACCAAGAGCTTGCAAATTGTTGCTGGAACC250.2442121715346293No Hit
GGCACTAGAAGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA210.20513822408908858No Hit
GGCACAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA200.19536973722770343No Hit
AAATACCGTTCAGAGCTGCCATGTCATGAGTGGCCAGATAGAGAGGCCCA200.19536973722770343No Hit
GGCACTAGAATGGACTCTTCCTCATCACTACATTATTTCAATTAAATACA190.18560125036631825No Hit
GGCATAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA190.18560125036631825No Hit
GGCACTAGAATGGCCCTCTTCCTCATCACTACATTATTTCAATTAAATAC180.1758327635049331No Hit
AAATACGTTCAGAGCTGCCACCAAGAGCTTGCAAATTGCTGCTGGAACCT160.15629578978216274No Hit
GGCCTAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA150.14652730292077756No Hit
AAATACCGTTCAGAGCTGCCCCCAAGAGCTTGCAAATTGCTGCTGGAACC150.14652730292077756No Hit
AAATACCGTTCAGAGCTGCCACAGAAACACACACACATTACGCTGTAGAA150.14652730292077756No Hit
GGCACTAGAATGGACCCTTCCTCATCACTACATTATTTCAATTAAATACA140.1367588160593924No Hit
GGCACTAGAATGGACCCCTTCCTCATCACTACATTATTTCAATTAAATAC140.1367588160593924No Hit
AAATACCGTTCAGAGCGCCACCAAGAGCTTGCAAATTGCTGCTGGAACCT130.12699032919800723No Hit
GGCACTAGAATGGACCTCTCCCTCATCACTACATTATTTCAATTAAATAC120.11722184233662206No Hit
GGCACTAGAATGGACCTCTTCCCCATCACTACATTATTTCAATTAAATAC110.10745335547523689No Hit
AAATACCGTTCAGAGCTGCCACCAAGAGCTTGCAAATTGCTGCTGGACCC110.10745335547523689No Hit
AATACCGTTCAGAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGAACCT110.10745335547523689No Hit
GGCACTAGAATGGACCTCTTACTCATCACTACATTATTTCAATTAAATAC110.10745335547523689No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)