FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008152174

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008152174
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11506
Total Bases1.6 Mbp
Sequences flagged as poor quality0
Sequence length142-145
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCACTAGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATAC305226.525291152442204No Hit
AAATACCGTTCAGAGCTGCCACCAAGAGCTTGCAAATTGCTGCTGGAACC251921.892925430210326No Hit
GGCACTAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATAC191016.60003476447071No Hit
AAATACCGTTCAGAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGAACC11339.847036328871893No Hit
AAATACCGTTCAGAGCTGCCTTGTTTTTAAGTTTACGCTGTAGAACCATG4433.850165131235877No Hit
AAATACCGTTCAGAACTGCCTTGTTTTTAAGTTTACGCTGTAGAACCATG890.7735094733182687No Hit
GGCACTAGATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA690.5996871197636016No Hit
GCACTAGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA520.45193811924213456No Hit
GGCACTAGATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA350.30418911872066745No Hit
AAATACCGTTCAGCACTGCCTTGTTTTTAAGTTTACGCTGTAGAACCATG350.30418911872066745No Hit
GGCACTAGAATGGACCTCTTCGTTGATAGCACTGCCTTGTTTTTAAGTTT350.30418911872066745No Hit
GGCACTAGAATGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA330.28680688336520077No Hit
AAATACCGTTCAGAGCTGCCAGCTTGGGGTACCTGGGAGGGCACAGCACT320.27811576568746743No Hit
GGCACTAAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA310.26942464800973404No Hit
GCACTAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA290.25204241265426736No Hit
AATACCGTTCAGAGCTGCCACCAAGAGCTTGCAAATTGCTGCTGGAACCT280.243351294976534No Hit
AAATACCGTTCAGAGCTGCCAGCTTGGGGTACCTGGGAGGGCATAGCACT260.22596905962106728No Hit
GGCACTGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA220.19120458891013384No Hit
GGCACTAGAATGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA210.1825134712324005No Hit
GGACTAGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA190.16513123587693376No Hit
AAATACGTTCAGAGCTGCCACCAAGAGCTTGCAAATTGCTGCTGGAACCT180.15644011819920042No Hit
GGACTAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA170.14774900052146706No Hit
GGCACTAGAAGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA170.14774900052146706No Hit
GGCCTAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA160.13905788284373372No Hit
GGCATAGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA160.13905788284373372No Hit
AAATACCGTTAGAGCTGCCACCAAGAGCTTGCAAATTGCTGCTGGAACCT160.13905788284373372No Hit
GGCACTAGAATGGCCCTCTTCCTCATCACTAAATTATTTCAATTAAATAC160.13905788284373372No Hit
AATACCGTTCAGAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGAACCT150.13036676516600035No Hit
GGCACAGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA150.13036676516600035No Hit
GGCACTAGAAGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA140.121675647488267No Hit
GGCACTAGAATGGACTCTTCCTCATCACTAAATTATTTCAATTAAATACA140.121675647488267No Hit
AAATACCGTTCGAGCTGCCACCAAGAGCTTGCAAATTGCTGCTGGAACCT140.121675647488267No Hit
GGCACTAGAATGGACTCTTCCTCATCACTACATTATTTCAATTAAATACA120.10429341213280027No Hit
GGCACTAGAATGGACCCTTCCTCATCACTACATTATTTCAATTAAATACA120.10429341213280027No Hit
AAATACCGTTCAGAGCTGCCACCAAGAGCTTGCAAATTGCTGCTGGACCC120.10429341213280027No Hit
AAATACCGTTCAGAGCTGCCACAGAAACACACACACATTACGCTGTAGAA120.10429341213280027No Hit
GGCCTAGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA120.10429341213280027No Hit
GGCACTGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA120.10429341213280027No Hit
AAATACCGTTCAGAGCGCCACCAAGAGCTTGCAAATTGCTGCTGGAACCT120.10429341213280027No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)