FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008152176

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008152176
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11943
Total Bases1.7 Mbp
Sequences flagged as poor quality0
Sequence length135-145
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCACTAGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATAC336328.158754081888972No Hit
GGCACTAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATAC300125.127689860169138No Hit
AAATACCGTTCAGAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGAACC216418.119400485640124No Hit
AAATACCGTTCAGAGCTGCCTTGTTTTTAAGTTTACGCTGTAGAACCATG5894.931759189483379No Hit
AAATACCGTTCAGAACTGCCTTGTTTTTAAGTTTACGCTGTAGAACCATG1801.5071590052750565No Hit
GGCACTAGATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA850.7117139747132212No Hit
AAATACCGTTCAGCACTGCCTTGTTTTTAAGTTTACGCTGTAGAACCATG670.5609980741857156No Hit
GCACTAGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA640.5358787574311312No Hit
GGCACTAGATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA630.5275056518462697No Hit
GCACTAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA480.40190906807334836No Hit
AAATACCGTTCAGAGCTGCCAGCTTGGGGTACCTGGGAGGGCACAGCACT380.31817801222473413No Hit
GGCACTAGAATGGACCTCTTCGTTGATAGCACTGCCTTGTTTTTAAGTTT370.3098049066398727No Hit
GGCACTAAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA350.29305869547014984No Hit
GGCACTAGAAGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA350.29305869547014984No Hit
GGCACTAGAATGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA330.27631248430042704No Hit
GGCACTGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA320.2679393787155656No Hit
GGACTAGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA290.24282006196098133No Hit
GGCACTAAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA280.23444695637611987No Hit
GGCACTAGAATGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA280.23444695637611987No Hit
AATACCGTTCAGAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGAACCT270.22607385079125847No Hit
AAATACGTTCAGAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGAACCT230.19258142845181278No Hit
GGCACAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA220.18420832286695135No Hit
GGCACTAGAATGGACTCTTCCTCATCACTAAATTATTTCAATTAAATACA200.1674621116972285No Hit
GGCACTGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA200.1674621116972285No Hit
GGCATAGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA190.15908900611236707No Hit
AAATACCGTTCAGAGCTGCCACAGAAACACACACACATTACGCTGTAGAA190.15908900611236707No Hit
GGACTAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA180.15071590052750566No Hit
GGCACTAGAAGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA180.15071590052750566No Hit
GGCACAGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA180.15071590052750566No Hit
AAATACCGTTCAGAGCTGCCAGCTTGGGGTACCTGGGAGGGCATAGCACT170.14234279494264424No Hit
AAATACCGTTAGAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGAACCT160.1339696893577828No Hit
GGCACTAGAATGGACTCTTCCTCATCACTACATTATTTCAATTAAATACA160.1339696893577828No Hit
GGCACTAGAATGGCCCTCTTCCTCATCACTAAATTATTTCAATTAAATAC160.1339696893577828No Hit
AAATACCGTTCAGAGCTGCCACCAGGAGCTTGCAAATTGATGCTGGAACC150.12559658377292138No Hit
AAATACCGTTCAGAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGACCC150.12559658377292138No Hit
GGCACTAGAATGGACCTCTTCCCCATCACTAAATTATTTCAATTAAATAC130.10885037260319852No Hit
GGCACTAGAATGGACCCTTCCTCATCACTAAATTATTTCAATTAAATACA130.10885037260319852No Hit
AATACCGTTCAGAGCTGCCTTGTTTTTAAGTTTACGCTGTAGAACCATGT130.10885037260319852No Hit
GGCACTAGAATGGACCTCTTCGTTGATACCACTGCCTTGTTTTTAAGTTT130.10885037260319852No Hit
AAATACCGTTCAGAGCGCCACCAGGAGCTTGCAAATTGCTGCTGGAACCT120.10047726701833709No Hit
GGCCTAGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA120.10047726701833709No Hit
GGCACTAGAATGGACCTCTCCCTCATCACTAAATTATTTCAATTAAATAC120.10047726701833709No Hit
GGCATAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA120.10047726701833709No Hit
GGCACTAGAATGGACCTCTTCCTCATCACTAAATTATTTAAATTAAATAC120.10047726701833709No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)