FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008152204

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008152204
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12265
Total Bases1.7 Mbp
Sequences flagged as poor quality0
Sequence length141-145
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAATACCGTTCAGAGCTGCCACCAAGAGCTTGCAAATTGCTGCTGGAACC283323.098247044435386No Hit
GGCACTAGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATAC279522.788422339991847No Hit
GGCACTAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATAC275122.429677945373015No Hit
AAATACCGTTCAGAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGAACC6195.046881369751325No Hit
AAATACCGTTCAGAGCTGCCTTGTTTTTAAGTTTACGCTGTAGAACCATG5014.084794129637179No Hit
AAATACCGTTCAGAACTGCCTTGTTTTTAAGTTTACGCTGTAGAACCATG1220.9947003668976764No Hit
AAATACCGTTCAGAGCTGCCAGCTTGGGGTACCTGGGAGGGCACAGCACT1030.839788014675907No Hit
GGCACTAGATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA590.48104362005707296No Hit
GGCACTAGATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA550.4484304932735426No Hit
GCACTAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA520.4239706481858948No Hit
GCACTAGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA500.40766408479412963No Hit
AAATACCGTTCAGAGCTGCCAGCTTGGGGTACCTGGGAGGGCATAGCACT500.40766408479412963No Hit
GGCACTAGAATGGACCTCTTCGTTGATAGCACTGCCTTGTTTTTAAGTTT500.40766408479412963No Hit
GGCACTAGAATGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA390.31797798613942113No Hit
AAATACCGTTCAGCACTGCCTTGTTTTTAAGTTTACGCTGTAGAACCATG390.31797798613942113No Hit
AATACCGTTCAGAGCTGCCACCAAGAGCTTGCAAATTGCTGCTGGAACCT360.2935181410517733No Hit
GGCACTAGAATGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA270.22013860578883002No Hit
GGCACTAAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA270.22013860578883002No Hit
GGACTAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA250.20383204239706482No Hit
AAATACGTTCAGAGCTGCCACCAAGAGCTTGCAAATTGCTGCTGGAACCT230.18752547900529964No Hit
GGCACTAGAAGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA220.17937219730941703No Hit
GGCACTGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA210.17121891561353444No Hit
GGACTAGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA200.16306563391765186No Hit
GGCACAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA190.15491235222176927No Hit
AAATACCGTTCAAGCTGCCACCAAGAGCTTGCAAATTGCTGCTGGAACCT190.15491235222176927No Hit
GGCACTAAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA190.15491235222176927No Hit
GGCACAGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA190.15491235222176927No Hit
GGCACTAGAATGGACTCTTCCTCATCACTACATTATTTCAATTAAATACA180.14675907052588666No Hit
AAATACCGTTCAGAGCTGCCACCAAGAGCTTGCAAATTGCTGCTGGACCC180.14675907052588666No Hit
GGCACTGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA170.13860578883000407No Hit
GGCATAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA170.13860578883000407No Hit
GGCCTAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA160.1304525071341215No Hit
AAATACCGTCAGAGCTGCCACCAAGAGCTTGCAAATTGCTGCTGGAACCT160.1304525071341215No Hit
AAATACCTTCAGAGCTGCCACCAAGAGCTTGCAAATTGCTGCTGGAACCT160.1304525071341215No Hit
AAATACCGTTCAGAGCTGCCACCAAGAGCTTGCAAATTGATGCTGGAACC150.12229922543823889No Hit
GGCACTAGAAGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA150.12229922543823889No Hit
GGCACTAGAATGGACCTCTCCCTCATCACTAAATTATTTCAATTAAATAC140.11414594374235629No Hit
AAATACCGTTCAGAGCTGCCCCCAAGAGCTTGCAAATTGCTGCTGGAACC130.1059926620464737No Hit
AAATACCGTTAGAGCTGCCACCAAGAGCTTGCAAATTGCTGCTGGAACCT130.1059926620464737No Hit
GGCACTAGAATGGCCCTCTTCCTCATCACTAAATTATTTCAATTAAATAC130.1059926620464737No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)