FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008152210

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008152210
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14676
Total Bases2.1 Mbp
Sequences flagged as poor quality0
Sequence length140-145
%GC36

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCACTAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATAC607841.41455437448896No Hit
GGCACTAGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATAC245116.70073589533933No Hit
AAATACCGTTCAGAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGAACC205213.982011447260835No Hit
AAATACCGTTCAGAGCTGCCTTGTTTTTAAGTTTACGCTGTAGAACCATG5573.7953120741346416No Hit
AAATACCGTTCAGAGCTGCCACCAAGAGCTTGCAAATTGCTGCTGGAACC5293.6045243935677296No Hit
AAATACCGTTCAGAACTGCCTTGTTTTTAAGTTTACGCTGTAGAACCATG1511.0288907059144181No Hit
GGCACTAGATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA1330.9062414826928319No Hit
GCACTAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA750.5110384300899428No Hit
AAATACCGTTCAGAGCTGCCAGCTTGGGGTACCTGGGAGGGCACAGCACT660.44971381847914965No Hit
GGCACTAGAATGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA600.4088307440719542No Hit
GGCACTAGATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA540.3679476696647588No Hit
GGCACTAAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA530.3611338239302262No Hit
AAATACCGTTCAGCACTGCCTTGTTTTTAAGTTTACGCTGTAGAACCATG510.3475061324611611No Hit
GCACTAGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA490.3338784409920959No Hit
GGCACTGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA490.3338784409920959No Hit
GGCACTAGAAGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA430.2929953665849005No Hit
GGACTAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA390.26573998364677026No Hit
GGCATAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA340.23167075497410738No Hit
GGCACTAGAATGGACTCTTCCTCATCACTACATTATTTCAATTAAATACA290.19760152630144456No Hit
GGCACAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA270.1839738348323794No Hit
GGCACTAGAATGGACCCTTCCTCATCACTACATTATTTCAATTAAATACA250.17034614336331425No Hit
AATACCGTTCAGAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGAACCT250.17034614336331425No Hit
GGCCTAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA230.1567184518942491No Hit
GGCACTAGAATGGCCCTCTTCCTCATCACTACATTATTTCAATTAAATAC210.14309076042518395No Hit
AAATACCGTTCAGAGCTGCCAGCTTGGGGTACCTGGGAGGGCATAGCACT200.1362769146906514No Hit
GGCACTAGAATGGACCTCTTCGTTGATAGCACTGCCTTGTTTTTAAGTTT190.12946306895611884No Hit
GGCACTAAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA170.11583537748705369No Hit
AAATACCGTCAGAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGAACCT170.11583537748705369No Hit
GGCACTAGAATGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA160.10902153175252113No Hit
AAATACCGTTCAAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGAACCT160.10902153175252113No Hit
GGCATAGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA150.10220768601798855No Hit
GGACTAGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA150.10220768601798855No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)