FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008152228

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008152228
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11699
Total Bases1.6 Mbp
Sequences flagged as poor quality0
Sequence length142-145
%GC36

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCACTAGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATAC430036.75527822890846No Hit
AAATACCGTTCAGAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGAACC233919.993161808701597No Hit
GGCACTAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATAC194016.582613898623812No Hit
AAATACCGTTCAGAGCTGCCTTGTTTTTAAGTTTACGCTGTAGAACCATG4734.04308060517993No Hit
AAATACCGTTCAGAACTGCCTTGTTTTTAAGTTTACGCTGTAGAACCATG1231.0513719121292417No Hit
GGCACTAGATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA1060.9060603470382085No Hit
GCACTAGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA750.6410804342251475No Hit
GGCACTAGAATGGACCTCTTCGTTGATAGCACTGCCTTGTTTTTAAGTTT510.43593469527310025No Hit
AAATACCGTTCAGCACTGCCTTGTTTTTAAGTTTACGCTGTAGAACCATG500.4273869561500983No Hit
GGCACTAGATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA490.4188392170270963No Hit
GGACTAGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA420.35900504316608256No Hit
GGCACTAGAATGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA410.3504573040430806No Hit
GGCACTAAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA390.3333618257970767No Hit
GCACTAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA380.3248140866740747No Hit
GGCACTGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA360.3077186084280708No Hit
GGCACAGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA340.2906231301820668No Hit
AATACCGTTCAGAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGAACCT300.25643217369005894No Hit
GGCCTAGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA260.2222412171980511No Hit
GGCACTAGAAGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA260.2222412171980511No Hit
AAATACCGTTCAGAGCTGCCAGCTTGGGGTACCTGGGAGGGCACAGCACT250.21369347807504915No Hit
GGCATAGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA230.19659799982904522No Hit
AAATACCGTTCAGAGCTGCCACCAAGAGCTTGCAAATTGCTGCTGGAACC190.16240704333703734No Hit
GGCACTAGAATGGACTCTTCCTCATCACTAAATTATTTCAATTAAATACA180.1538593042140354No Hit
AAATACCGTTCAGAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGACCC180.1538593042140354No Hit
GGCACTAAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA170.1453115650910334No Hit
GGCACAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA150.12821608684502947No Hit
GGCACTAGAATGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA150.12821608684502947No Hit
AAATACGTTCAGAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGAACCT140.11966834772202753No Hit
GGCACTAGAATGGCCTCTTCCTCATCACTAAATTATTTCAATTAAATACA140.11966834772202753No Hit
GGCACTAGAATGGACCTCTCCCTCATCACTAAATTATTTCAATTAAATAC140.11966834772202753No Hit
AAATACCGTTCAAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGAACCT140.11966834772202753No Hit
GGCACTAGAATGGACTCTTCCTCATCACTACATTATTTCAATTAAATACA130.11112060859902555No Hit
GGCACTAGAATGGCCCTCTTCCTCATCACTAAATTATTTCAATTAAATAC130.11112060859902555No Hit
GGACTAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA120.10257286947602359No Hit
AAATACCGTTCAGGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGAACCT120.10257286947602359No Hit
GGCACTAGAATGGACCCTTCCTCATCACTAAATTATTTCAATTAAATACA120.10257286947602359No Hit
GGCACTGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA120.10257286947602359No Hit
GGCACTAGAATGGACCTCTTACTCATCACTAAATTATTTCAATTAAATAC120.10257286947602359No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)