FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008152238

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008152238
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11396
Total Bases1.6 Mbp
Sequences flagged as poor quality0
Sequence length141-145
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCACTAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATAC519145.55107055107055No Hit
AAATACCGTTCAGAGCTGCCACCAAGAGCTTGCAAATTGCTGCTGGAACC224919.734994734994736No Hit
AAATACCGTTCAGAGCTGCCTTGTTTTTAAGTTTACGCTGTAGAACCATG7496.572481572481573No Hit
AAATACCGTTCAGAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGAACC4894.2909792909792905No Hit
AAATACCGTTCAGAACTGCCTTGTTTTTAAGTTTACGCTGTAGAACCATG1991.7462267462267465No Hit
GGCACTAGATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA1100.9652509652509652No Hit
GCACTAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA820.7195507195507196No Hit
AAATACCGTTCAGAGCTGCCAGCTTGGGGTACCTGGGAGGGCACAGCACT750.6581256581256582No Hit
AAATACCGTTCAGCACTGCCTTGTTTTTAAGTTTACGCTGTAGAACCATG720.6318006318006317No Hit
GGCACTAAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA440.3861003861003861No Hit
GGCACTAGAATGGACCTCTTCGTTGATAGCACTGCCTTGTTTTTAAGTTT430.37732537732537735No Hit
GGCACTAGAATGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA400.35100035100035104No Hit
GGCACTGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA390.34222534222534223No Hit
GGCACTAGAAGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA370.3246753246753247No Hit
AAATACCGTTCAGAGCTGCCAGCTTGGGGTACCTGGGAGGGCATAGCACT340.29835029835029836No Hit
GGCATAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA290.2544752544752545No Hit
GGACTAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA250.21937521937521937No Hit
GGCCTAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA250.21937521937521937No Hit
GGCACTAGAATGGACTCTTCCTCATCACTACATTATTTCAATTAAATACA250.21937521937521937No Hit
GGCACAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA240.2106002106002106No Hit
GGCACTAGAATGGACCCTTCCTCATCACTACATTATTTCAATTAAATACA240.2106002106002106No Hit
AATACCGTTCAGAGCTGCCACCAAGAGCTTGCAAATTGCTGCTGGAACCT200.17550017550017552No Hit
AAATACGTTCAGAGCTGCCACCAAGAGCTTGCAAATTGCTGCTGGAACCT190.16672516672516674No Hit
AAATACCGTTCAGAGCTGCCTTGTTTTAAGTTTACGCTGTAGAACCATGT180.15795015795015793No Hit
AAATACCGTTCAAGCTGCCACCAAGAGCTTGCAAATTGCTGCTGGAACCT170.14917514917514918No Hit
GGCACTAGAATGGCCCTCTTCCTCATCACTACATTATTTCAATTAAATAC170.14917514917514918No Hit
GGCACTAGAATGGACCTCTCCCTCATCACTACATTATTTCAATTAAATAC170.14917514917514918No Hit
AAATACCGTTCAGAGCTGCCACAGAAACACACACACATTACGCTGTAGAA160.1404001404001404No Hit
AAATACCGTTCAGAGCTGCCATGTCATGAGTGGCCAGATAGAGAGGCCCA160.1404001404001404No Hit
AAATACCGTTCAGAGCTGCCCCCAAGAGCTTGCAAATTGCTGCTGGAACC150.13162513162513162No Hit
AAATACCGTCAGAGCTGCCACCAAGAGCTTGCAAATTGCTGCTGGAACCT140.12285012285012285No Hit
GGCACTAGAATGGACCTCTTCCCCATCACTACATTATTTCAATTAAATAC130.11407511407511407No Hit
GGCACTAGAATGGACCTCTTCCTCATCACTACGTTATTTCAATTAAATAC130.11407511407511407No Hit
AATACCGTTCAGAGCTGCCTTGTTTTTAAGTTTACGCTGTAGAACCATGT130.11407511407511407No Hit
GGCACTAGAATGGCCTCTTCCTCATCACTACATTATTTCAATTAAATACA130.11407511407511407No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)