FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008152246

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008152246
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12111
Total Bases1.7 Mbp
Sequences flagged as poor quality0
Sequence length141-145
%GC35

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCACTAGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATAC639652.81149368342829No Hit
AAATACCGTTCAGAGCTGCCACCAAGAGCTTGCAAATTGCTGCTGGAACC211417.455206011064323No Hit
GGCACTAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATAC5534.566096936669144No Hit
AAATACCGTTCAGAGCTGCCTTGTTTTTAAGTTTACGCTGTAGAACCATG2762.2789199900916524No Hit
GGCACTAGATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA1271.0486334737015937No Hit
GCACTAGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA1090.9000082569564858No Hit
AAATACCGTTCAGAACTGCCTTGTTTTTAAGTTTACGCTGTAGAACCATG1020.8422095615556106No Hit
GGCACTAGAATGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA720.594500866980431No Hit
AAATACCGTTCAGAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGAACC720.594500866980431No Hit
AAATACCGTTCAGAGCTGCCAGCTTGGGGTACCTGGGAGGGCATAGCACT650.5367021715795558No Hit
AAATACCGTTCAGAGCTGCCAGCTTGGGGTACCTGGGAGGGCACAGCACT620.5119313021220379No Hit
GGCACTAAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA530.4376186937494839No Hit
GGCATAGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA470.38807695483444804No Hit
GGACTAGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA450.3715630418627694No Hit
GGCACTGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA450.3715630418627694No Hit
AAATACCGTTCAGCACTGCCTTGTTTTTAAGTTTACGCTGTAGAACCATG450.3715630418627694No Hit
GGCACTAGAAGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA420.3467921724052514No Hit
GGCACTAGAATGGACCCTTCCTCATCACTAAATTATTTCAATTAAATACA340.28073652051853687No Hit
GGCACTAGAATGGACCTCTTCGTTGATAGCACTGCCTTGTTTTTAAGTTT340.28073652051853687No Hit
GGCACAGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA320.2642226075468582No Hit
AATACCGTTCAGAGCTGCCACCAAGAGCTTGCAAATTGCTGCTGGAACCT310.25596565106101893No Hit
GGCCTAGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA290.23945173808934028No Hit
GGCACTAGAATGGACTCTTCCTCATCACTAAATTATTTCAATTAAATACA270.2229378251176616No Hit
GGCACTAGAATGGACCTCTTCCCCATCACTAAATTATTTCAATTAAATAC240.19816695566014364No Hit
GGCACTAGAATGGCCCTCTTCCTCATCACTAAATTATTTCAATTAAATAC200.1651391297167864No Hit
AAATACGTTCAGAGCTGCCACCAAGAGCTTGCAAATTGCTGCTGGAACCT190.15688217323094705No Hit
GGCACTAGAATGGACCTCTCCCTCATCACTAAATTATTTCAATTAAATAC190.15688217323094705No Hit
GGCACTAGAATGGACCTCTTCCTCACCACTAAATTATTTCAATTAAATAC170.14036826025926843No Hit
GGCACTAGAATGGACCTCTTCCTCATCACTAAATTATTTAAATTAAATAC170.14036826025926843No Hit
GGCACTAGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATGC160.1321113037734291No Hit
AAATACCGTTCAGAGCGCCACCAAGAGCTTGCAAATTGCTGCTGGAACCT140.11559739080175048No Hit
GGCACTAGAATGGACCTCTTCCTCATCGCTAAATTATTTCAATTAAATAC130.10734043431591116No Hit
AAATACCGTTCAGAGCTGCCACCAAGAGCTTGCAAATTGCTGCTGGACCC130.10734043431591116No Hit
GGCACTAGAATGGACCTCTTCTCATCACTAAATTATTTCAATTAAATACA130.10734043431591116No Hit
GGCACTAGAATGGACCTCTTACTCATCACTAAATTATTTCAATTAAATAC130.10734043431591116No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)