FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008152248

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008152248
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14477
Total Bases2 Mbp
Sequences flagged as poor quality0
Sequence length141-145
%GC32

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCACTAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATAC641844.3323893071769No Hit
GGCACTAGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATAC435530.082199350694204No Hit
AAATACCGTTCAGAGCTGCCTTGTTTTTAAGTTTACGCTGTAGAACCATG2401.6578020308074877No Hit
AAATACCGTTCAGAGCTGCCAGCTTGGGGTACCTGGGAGGGCACAGCACT1461.0084962354078884No Hit
GGCACTAGATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA1440.9946812184844926No Hit
AAATACCGTTCAGAGCTGCCAGCTTGGGGTACCTGGGAGGGCATAGCACT1170.8081784900186504No Hit
GCACTAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA1000.6907508461697865No Hit
GGCACTAGATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA890.61476825309111No Hit
GCACTAGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA760.5249706430890377No Hit
AAATACCGTTCAGAACTGCCTTGTTTTTAAGTTTACGCTGTAGAACCATG670.46280306693375695No Hit
GGCACTGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA600.4144505077018719No Hit
GGCACTAGAATGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA580.40063549077847627No Hit
GGACTAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA480.33156040616149757No Hit
GGCACAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA440.3039303723147061No Hit
GGCACTAAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA440.3039303723147061No Hit
GGCACTAGAAGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA420.29011535539131034No Hit
AAATACCGTTCAGCACTGCCTTGTTTTTAAGTTTACGCTGTAGAACCATG420.29011535539131034No Hit
GGCACTAGAATGGACCTCTTCGTTGATAGCACTGCCTTGTTTTTAAGTTT400.27630033846791463No Hit
GGCACTAAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA390.2693928300062168No Hit
GGCACTAGAATGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA380.26248532154451887No Hit
GGCACTGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA380.26248532154451887No Hit
GGCACTAGAATGGACTCTTCCTCATCACTACATTATTTCAATTAAATACA320.2210402707743317No Hit
GGACTAGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA310.21413276231263384No Hit
GGCACAGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA310.21413276231263384No Hit
GGCATAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA300.20722525385093596No Hit
GGCCTAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA260.1795952200041445No Hit
GGCACTAGAAGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA260.1795952200041445No Hit
GGCACTAGAATGGACCCTTCCTCATCACTACATTATTTCAATTAAATACA220.15196518615735305No Hit
GGCATAGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA220.15196518615735305No Hit
GGCACTAGAATGGCCCTCTTCCTCATCACTACATTATTTCAATTAAATAC200.13815016923395732No Hit
GGCCTAGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA200.13815016923395732No Hit
GGCACTAGAATGGACCTCTCCCTCATCACTACATTATTTCAATTAAATAC200.13815016923395732No Hit
GGCACTAGAATGGCCTCTTCCTCATCACTACATTATTTCAATTAAATACA190.13124266077225943No Hit
GGCACTAGAATGGACCCCTTCCTCATCACTACATTATTTCAATTAAATAC180.12433515231056158No Hit
GGCACTAGAATGGACCCTTCCTCATCACTAAATTATTTCAATTAAATACA180.12433515231056158No Hit
GGCACTAGAATGGACTCTTCCTCATCACTAAATTATTTCAATTAAATACA160.11052013538716585No Hit
GGCACTAGAATGGCCCTCTTCCTCATCACTAAATTATTTCAATTAAATAC160.11052013538716585No Hit
GGCACTAGAATGGACCTCTTCGTCATCACTACATTATTTCAATTAAATAC150.10361262692546798No Hit
GGCACTAGAATGGACCTCTTCCTCATCGCTACATTATTTCAATTAAATAC150.10361262692546798No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)