FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008152260

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008152260
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12108
Total Bases1.7 Mbp
Sequences flagged as poor quality0
Sequence length142-145
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCACTAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATAC389732.18533201189297No Hit
AAATACCGTTCAGAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGAACC207817.162206805417906No Hit
GGCACTAGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATAC175214.469772051536175No Hit
AAATACCGTTCAGAGCTGCCACCAAGAGCTTGCAAATTGCTGCTGGAACC12049.94383878427486No Hit
AAATACCGTTCAGAGCTGCCTTGTTTTTAAGTTTACGCTGTAGAACCATG3763.1053848695077635No Hit
GGCACTAGATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA1030.8506772381896267No Hit
AAATACCGTTCAGAGCTGCCAGCTTGGGGTACCTGGGAGGGCACAGCACT1020.8424182358771061No Hit
AAATACCGTTCAGAACTGCCTTGTTTTTAAGTTTACGCTGTAGAACCATG980.8093822266270234No Hit
AAATACCGTTCAGAGCTGCCAGCTTGGGGTACCTGGGAGGGCATAGCACT710.586389164188966No Hit
GCACTAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA690.5698711595639246No Hit
GGCACTAGATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA480.39643211100099107No Hit
GGCACTAGAATGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA430.35513709943838784No Hit
GGCACTGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA380.3138420878757846No Hit
GCACTAGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA360.29732408325074333No Hit
AAATACCGTTCAGCACTGCCTTGTTTTTAAGTTTACGCTGTAGAACCATG360.29732408325074333No Hit
GGCACTAGAATGGACCTCTTCGTTGATAGCACTGCCTTGTTTTTAAGTTT360.29732408325074333No Hit
GGACTAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA350.28906508093822264No Hit
GGCACTAAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA350.28906508093822264No Hit
AATACCGTTCAGAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGAACCT270.22299306243805747No Hit
GGCATAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA260.2147340601255368No Hit
GGCACTAGAATGGACTCTTCCTCATCACTACATTATTTCAATTAAATACA250.2064750578130162No Hit
GGCACTAGAAGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA240.19821605550049554No Hit
AAATACCGTCAGAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGAACCT230.1899570531879749No Hit
GGCACAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA220.18169805087545426No Hit
AAATACGTTCAGAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGAACCT190.1569210439378923No Hit
GGCACTAGAATGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA180.14866204162537167No Hit
GGACTAGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA180.14866204162537167No Hit
GGCACTAAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA150.1238850346878097No Hit
GGCACTAGAATGGCCCTCTTCCTCATCACTACATTATTTCAATTAAATAC150.1238850346878097No Hit
GGCACTGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA150.1238850346878097No Hit
AAATACCGTTCAGAGCTGCCACAGAAACACACACACATTACGCTGTAGAA150.1238850346878097No Hit
AAATACCGTTCAGAGCTGCCACCAGGAGCTTGCAAATTGCTACTGGAACC140.11562603237528907No Hit
GGCACTAGAATGGACCTCTTCCTCATCACTACACTATTTCAATTAAATAC130.1073670300627684No Hit
AAATACCGTTCAGAGCTGCCACGGCGTCTCCAGACCCAGCTCCGGCCGGG130.1073670300627684No Hit
GGCACTAGAATGGACCTCTTCCCCATCACTACATTATTTCAATTAAATAC130.1073670300627684No Hit
AATACCGTTCAGAGCTGCCACCAAGAGCTTGCAAATTGCTGCTGGAACCT130.1073670300627684No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)