FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008152265

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008152265
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7902
Total Bases1.1 Mbp
Sequences flagged as poor quality0
Sequence length139-145
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACTTAAAAACAAGGCAGTGCTAATGCCTAATGGTGCTACAGTTTCTGCCT384648.6712224753227No Hit
TGTGTGTGTGTTTCTGTGGGTTTCTTTAAGGTTTGGACAGAAGGGTAAAG183223.184004049607694No Hit
ACTTAAAAACAAGGCAGCTCTGAACGGTATTTCCTCTAAGACCAAGTCTC6878.694001518602885No Hit
ACTTAAAAACAAGGCAGTTCTGAACGGTATTTCCTCTAAGACCAAGTCTC2022.5563148569982284No Hit
ACTTAAAACAAGGCAGTGCTAATGCCTAATGGTGCTACAGTTTCTGCCTC1141.442672741078208No Hit
ACTTAAAAACAAGGCAGTGCTGAACGGTATTTCCTCTAAGACCAAGTCTC811.0250569476082005No Hit
CTTAAAAACAAGGCAGTGCTAATGCCTAATGGTGCTACAGTTTCTGCCTC400.5062009617818274No Hit
ACTTAAAAACAGGCAGTGCTAATGCCTAATGGTGCTACAGTTTCTGCCTC380.480890913692736No Hit
ACTTAAAAACAAGGCAGTGCTATCAACGAAGAGGTCCATTCTAGTGCCCC370.46823588964819035No Hit
ACTTAAAAACAAGCAGTGCTAATGCCTAATGGTGCTACAGTTTCTGCCTC280.3543406732472792No Hit
ACTTAAAAACAAGGCAGTGCTGTGCCCTCCCAGGTACCCCAAGCTGGCAG270.3416856492027335No Hit
TGTGTGTGTGTTTCTGTGGCAGCTCTGAACGGTATTTCCTCTAAGACCAA170.21513540875727666No Hit
ACTTAAAACAAGGCAGCTCTGAACGGTATTTCCTCTAAGACCAAGTCTCT160.20248038471273097No Hit
ACTAAAAACAAGGCAGTGCTAATGCCTAATGGTGCTACAGTTTCTGCCTC160.20248038471273097No Hit
ACTTAAAAAAAGGCAGTGCTAATGCCTAATGGTGCTACAGTTTCTGCCTC150.18982536066818526No Hit
ATTAAAAACAAGGCAGTGCTAATGCCTAATGGTGCTACAGTTTCTGCCTC150.18982536066818526No Hit
ACTTAAAAACAAGGCAGTGCTAATGACTAATGGTGCTACAGTTTCTGCCT120.15186028853454822No Hit
ACTTAAAAACAAGGCGTGCTAATGCCTAATGGTGCTACAGTTTCTGCCTC120.15186028853454822No Hit
ACTTAAAAACAAGGCAGTGCCAATGCCTAATGGTGCTACAGTTTCTGCCT110.13920526449000253No Hit
ACTTAAAAACAAGGCAGTGCTAATGCCCAATGGTGCTACAGTTTCTGCCT110.13920526449000253No Hit
TGTGTGTGTGTTTCTGTGGGTTTGGGTGGGAAGAGGTCCATTCTAGTGCC110.13920526449000253No Hit
ACTTAAAAACAAGGCAGTGCTAATGCCTAATGGTGCTACAGTTTCTGCCC110.13920526449000253No Hit
ACTTAAAAACAAAGCAGTGGTATCAACGCAGAGGTCCATTCTAGTGCCCC100.12655024044545685Clontech Universal Primer Mix Long (95% over 22bp)
ACTTAAAAACAAGGCAGTGGTATCAACGAAGAGGTCCATTCTAGTGCCCC90.11389521640091116No Hit
ACTTAAAAACAAGGCAGTGCTATGCCCTCCCAGGTACCCCAAGCTGGCAG90.11389521640091116No Hit
ACTTAAAAACAAGGCAGTGCTAATGCCTAATGGTGCCACAGTTTCTGCCT90.11389521640091116No Hit
ACTTAAAAACAAGGCAGTGCTAACGCCTAATGGTGCTACAGTTTCTGCCT90.11389521640091116No Hit
ACTTAAAAACAAGGCAGTGCTAATGCCTAATGGTGCTACAGTTTCCGCCT90.11389521640091116No Hit
ACTTAAAAACAAGGCAGTGGTATCAACGCAGAGGTCCATTCTAGTGCCCC90.11389521640091116Clontech Universal Primer Mix Long (95% over 22bp)
TGTGTGTGTGTTCTGTGGGTTTCTTTAAGGTTTGGACAGAAGGGTAAAGC90.11389521640091116No Hit
ACTTAAAAACAAGGCAGTGCTAATGCCTAATGGTGCTACAGTTCCTGCCT80.10124019235636549No Hit
ACTTAAAAACAAGGCAGTGCTAATGCCTAATGGTGCTACAGTTTCTGTCT80.10124019235636549No Hit
ACTTAAAAACAAGGAGTGCTAATGCCTAATGGTGCTACAGTTTCTGCCTC80.10124019235636549No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)