FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008152270

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008152270
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11775
Total Bases1.7 Mbp
Sequences flagged as poor quality0
Sequence length141-145
%GC36

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCACTAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATAC370431.45647558386412No Hit
GGCACTAGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATAC255521.698513800424628No Hit
AAATACCGTTCAGAGCTGCCACCAAGAGCTTGCAAATTGCTGCTGGAACC198016.81528662420382No Hit
AAATACCGTTCAGAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGAACC5164.382165605095541No Hit
AAATACCGTTCAGAGCTGCCTTGTTTTTAAGTTTACGCTGTAGAACCATG4914.169851380042463No Hit
AAATACCGTTCAGAACTGCCTTGTTTTTAAGTTTACGCTGTAGAACCATG1160.9851380042462845No Hit
GGCACTAGATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA690.5859872611464968No Hit
AAATACCGTTCAGAGCTGCCAGCTTGGGGTACCTGGGAGGGCACAGCACT620.5265392781316348No Hit
GCACTAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA540.45859872611464964No Hit
GGCACTAGATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA530.45010615711252655No Hit
GGCACTAGAATGGACCTCTTCGTTGATAGCACTGCCTTGTTTTTAAGTTT500.42462845010615713No Hit
GCACTAGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA490.41613588110403393No Hit
AAATACCGTTCAGAGCTGCCAGCTTGGGGTACCTGGGAGGGCATAGCACT380.3227176220806794No Hit
AAATACCGTTCAGCACTGCCTTGTTTTTAAGTTTACGCTGTAGAACCATG370.31422505307855625No Hit
AATACCGTTCAGAGCTGCCACCAAGAGCTTGCAAATTGCTGCTGGAACCT370.31422505307855625No Hit
GGCACTAGAATGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA350.29723991507431No Hit
GGCACTGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA340.28874734607218683No Hit
GGCACTAAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA300.25477707006369427No Hit
GGCACAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA260.2208067940552017No Hit
GGCACTAAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA250.21231422505307856No Hit
GGACTAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA200.16985138004246286No Hit
GGCACTAGAATGGACCCTTCCTCATCACTACATTATTTCAATTAAATACA200.16985138004246286No Hit
GGCACTGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA200.16985138004246286No Hit
GGCACTAGAATGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA190.1613588110403397No Hit
GGCACTAGAATGGCCCTCTTCCTCATCACTACATTATTTCAATTAAATAC170.14437367303609341No Hit
GGACTAGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA170.14437367303609341No Hit
AAATACGTTCAGAGCTGCCACCAAGAGCTTGCAAATTGCTGCTGGAACCT170.14437367303609341No Hit
GGCATAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA160.13588110403397027No Hit
GGCCTAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA140.11889596602972399No Hit
GGCATAGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA140.11889596602972399No Hit
GGCACTAGAAGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA130.11040339702760085No Hit
GGCACTAGAATGGACTCTTCCTCATCACTACATTATTTCAATTAAATACA130.11040339702760085No Hit
AAATACCGTTCAGAGCTGCCACCAAGAGCTTGCAAATTGCTGCTGGACCC120.1019108280254777No Hit
GGCACAGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA120.1019108280254777No Hit
GGCACTAGAAGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA120.1019108280254777No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)