FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008152280

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008152280
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13004
Total Bases1.8 Mbp
Sequences flagged as poor quality0
Sequence length141-145
%GC36

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCACTAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATAC488837.58843432789911No Hit
GGCACTAGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATAC223417.17932943709628No Hit
AAATACCGTTCAGAGCTGCCACCAAGAGCTTGCAAATTGCTGCTGGAACC150411.565672100892034No Hit
AAATACCGTTCAGAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGAACC10838.328206705629038No Hit
AAATACCGTTCAGAGCTGCCTTGTTTTTAAGTTTACGCTGTAGAACCATG3422.6299600123039064No Hit
GGCACTAGATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA1080.8305136880959704No Hit
AAATACCGTTCAGAACTGCCTTGTTTTTAAGTTTACGCTGTAGAACCATG1030.7920639803137496No Hit
GCACTAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA860.6613349738541987No Hit
AAATACCGTTCAGCACTGCCTTGTTTTTAAGTTTACGCTGTAGAACCATG580.44601661027376194No Hit
GGCACTAGAATGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA540.4152568440479852No Hit
GGCACTGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA510.3921870193786527No Hit
GGCACTAAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA470.36142725315287605No Hit
GCACTAGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA460.3537373115964319No Hit
GGCACTAGATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA410.31528760381421106No Hit
GGACTAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA340.2614580129191018No Hit
GGCATAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA340.2614580129191018No Hit
GGCACAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA320.2460781298062135No Hit
GGCACTAGAATGGACCTCTTCGTTGATAGCACTGCCTTGTTTTTAAGTTT320.2460781298062135No Hit
GGCACTAGAAGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA280.2153183635804368No Hit
GGCACTAGAATGGACTCTTCCTCATCACTACATTATTTCAATTAAATACA280.2153183635804368No Hit
AATACCGTTCAGAGCTGCCACCAAGAGCTTGCAAATTGCTGCTGGAACCT240.1845585973546601No Hit
GGCACTAGAAGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA230.17686865579821595No Hit
GGACTAGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA220.16917871424177175No Hit
GGCCTAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA210.16148877268532758No Hit
GGCATAGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA210.16148877268532758No Hit
GGCACTAGAATGGACCTCTTCCCCATCACTACATTATTTCAATTAAATAC200.15379883112888343No Hit
GGCACAGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA200.15379883112888343No Hit
GGCACTAAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA190.14610888957243925No Hit
GGCACTGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA180.13841894801599508No Hit
GGCACTAGAATGGCCCTCTTCCTCATCACTACATTATTTCAATTAAATAC170.1307290064595509No Hit
AATACCGTTCAGAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGAACCT160.12303906490310675No Hit
GGCACTAGAATGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA150.11534912334666256No Hit
GGCACTAGAATGGACCTCTTCGTCATCACTACATTATTTCAATTAAATAC150.11534912334666256No Hit
GGCACTAGAATGGACCCTTCCTCATCACTACATTATTTCAATTAAATACA150.11534912334666256No Hit
GGCACTAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAACAC150.11534912334666256No Hit
GGCACTAGAATGGACCTCTCCCTCATCACTACATTATTTCAATTAAATAC150.11534912334666256No Hit
GGCACTAGAATGGACCTCTTAGCACTGCCTTGTTTTTAAGTTTACGCTGT140.1076591817902184No Hit
GGCCTAGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA140.1076591817902184No Hit
GGCACTAGAATGGACCTCTTCCTCACCACTACATTATTTCAATTAAATAC140.1076591817902184No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)