FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008152296

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008152296
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11804
Total Bases1.7 Mbp
Sequences flagged as poor quality0
Sequence length141-145
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCACTAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATAC403534.18332768553033No Hit
AAATACCGTTCAGAGCTGCCACCAAGAGCTTGCAAATTGCTGCTGGAACC313326.54185022026432No Hit
GGCACTAGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATAC9628.149779735682818No Hit
AAATACCGTTCAGAGCTGCCTTGTTTTTAAGTTTACGCTGTAGAACCATG5064.28668248051508No Hit
AAATACCGTTCAGAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGAACC3693.126058963063368No Hit
GGCACTAGATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA1231.0420196543544562No Hit
AAATACCGTTCAGAACTGCCTTGTTTTTAAGTTTACGCTGTAGAACCATG1150.9742460182988818No Hit
GGCACTAGAATGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA610.5167739749237547No Hit
GCACTAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA610.5167739749237547No Hit
AATACCGTTCAGAGCTGCCACCAAGAGCTTGCAAATTGCTGCTGGAACCT480.40664181633344626No Hit
GGCACTAAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA440.3727549983056591No Hit
GGCACTGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA410.3473398847848187No Hit
GGACTAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA390.3303964757709251No Hit
AAATACCGTTCAGCACTGCCTTGTTTTTAAGTTTACGCTGTAGAACCATG380.32192477126397834No Hit
GGCATAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA380.32192477126397834No Hit
GGCACTAGAATGGACTCTTCCTCATCACTACATTATTTCAATTAAATACA360.3049813622500847No Hit
AAATACCGTTCAGAGCTGCCACCAAGAGCTTGCAAATTGCTGCTGGACCC330.27956624872924435No Hit
GGCACTAGATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA310.2626228397153507No Hit
GGCACTAGAAGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA290.24567943070145712No Hit
GGCCTAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA280.23720772619451033No Hit
AAATACCGTCAGAGCTGCCACCAAGAGCTTGCAAATTGCTGCTGGAACCT260.22026431718061676No Hit
AAATACGTTCAGAGCTGCCACCAAGAGCTTGCAAATTGCTGCTGGAACCT250.21179261267366992No Hit
GGCACTAGAATGGACCTCTTCGTTGATAGCACTGCCTTGTTTTTAAGTTT230.19484920365977634No Hit
GCACTAGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA210.17790579464588274No Hit
GGCACTAGAATGGACCCTTCCTCATCACTACATTATTTCAATTAAATACA200.16943409013893596No Hit
GGCACAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA180.15249068112504235No Hit
AAATACCGTTCAGAGCTGCCCCCAAGAGCTTGCAAATTGCTGCTGGAACC170.14401897661809557No Hit
AAATACCGTTCAGAGCTGCCACAGAAACACACACACATTACGCTGTAGAA160.13554727211114878No Hit
AAATACCGTTCAGAGCTGCCACCAAGAGCTTGCAAATTGCTGCCGGAACC150.12707556760420197No Hit
GGCACTAGAATGGACCTCTTCTTCATCACTACATTATTTCAATTAAATAC140.11860386309725517No Hit
GGCACTAGAATGGACCTCTTCCCCATCACTACATTATTTCAATTAAATAC140.11860386309725517No Hit
GGCACTAGAATGGACCTCTCCCTCATCACTACATTATTTCAATTAAATAC140.11860386309725517No Hit
AAATACCGTTCAGAGCGCCACCAAGAGCTTGCAAATTGCTGCTGGAACCT140.11860386309725517No Hit
AAATACCGTTCAAGCTGCCACCAAGAGCTTGCAAATTGCTGCTGGAACCT130.11013215859030838No Hit
AAATACCGTTCAGAGCTGCCACCAAGGGCTTGCAAATTGCTGCTGGAACC130.11013215859030838No Hit
AAATACCGTTCAGAGCTGCCACCAAGAGCTTGCAAATTGATGCTGGAACC130.11013215859030838No Hit
AAATACCGTTCAGAGCTGCCAGCTTGGGGTACCTGGGAGGGCACAGCACT130.11013215859030838No Hit
AAATACCGTTAGAGCTGCCACCAAGAGCTTGCAAATTGCTGCTGGAACCT120.10166045408336156No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)