FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008152298

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008152298
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13029
Total Bases1.8 Mbp
Sequences flagged as poor quality0
Sequence length141-145
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCACTAGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATAC441933.91664747870136No Hit
GGCACTAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATAC224617.238468032849795No Hit
AAATACCGTTCAGAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGAACC158812.188195563742422No Hit
AAATACCGTTCAGAGCTGCCACCAAGAGCTTGCAAATTGCTGCTGGAACC7285.5875354977358205No Hit
AAATACCGTTCAGAGCTGCCTTGTTTTTAAGTTTACGCTGTAGAACCATG6565.034922096860849No Hit
AAATACCGTTCAGAGCTGCCAGCTTGGGGTACCTGGGAGGGCACAGCACT1581.2126794074756313No Hit
AAATACCGTTCAGAACTGCCTTGTTTTTAAGTTTACGCTGTAGAACCATG1491.1436027323662599No Hit
GGCACTAGATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA1280.9824238237777265No Hit
GCACTAGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA1000.7675186123263489No Hit
AAATACCGTTCAGAGCTGCCAGCTTGGGGTACCTGGGAGGGCATAGCACT970.7444930539565585No Hit
AAATACCGTTCAGCACTGCCTTGTTTTTAAGTTTACGCTGTAGAACCATG630.4835367257655998No Hit
GGCACTAGATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA580.44516079514928236No Hit
GGCACTAGAATGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA570.4374856090260189No Hit
GGCACTAAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA550.4221352367794919No Hit
GCACTAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA520.3991096784097014No Hit
GGACTAGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA470.360733747793384No Hit
GGCACAGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA450.345383375546857No Hit
GGCACTGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA390.2993322588072761No Hit
GGCACTAGAATGGACCTCTTCGTTGATAGCACTGCCTTGTTTTTAAGTTT380.2916570726840126No Hit
GGCATAGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA350.2686315143142221No Hit
GGCACTAGAATGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA340.26095632819095865No Hit
GGCACTGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA300.2302555836979047No Hit
GGCACTAGAAGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA280.21490521145137767No Hit
GGCACTAAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA270.20723002532811421No Hit
AATACCGTTCAGAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGAACCT260.1995548392048507No Hit
GGCCTAGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA250.19187965308158722No Hit
GGCACTAGAATGGACTCTTCCTCATCACTAAATTATTTCAATTAAATACA240.18420446695832374No Hit
GGCACTAGAATGGCCTCTTCCTCATCACTAAATTATTTCAATTAAATACA230.17652928083506025No Hit
GGACTAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA210.16117890858853326No Hit
GGCACTAGAATGGACCCTTCCTCATCACTAAATTATTTCAATTAAATACA210.16117890858853326No Hit
GGCACTAGAATGGACCTCTTCCTCACCACTAAATTATTTCAATTAAATAC200.15350372246526978No Hit
GGCACTAGAATGGCCCTCTTCCTCATCACTAAATTATTTCAATTAAATAC190.1458285363420063No Hit
GGCATAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA190.1458285363420063No Hit
GGCACTAGAATGGACCTCTCCCTCATCACTAAATTATTTCAATTAAATAC180.1381533502187428No Hit
GGCACAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA170.13047816409547933No Hit
GGCCTAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA160.12280297797221582No Hit
GGCACTAGAAGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA160.12280297797221582No Hit
AAATACCGTTCAGAGCTGCCACAGAAACACACACACATTACGCTGTAGAA160.12280297797221582No Hit
GGCACTAGAATGGACCTCTTCCTCATCACTAAATCATTTCAATTAAATAC150.11512779184895235No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)