FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008152303

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008152303
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11645
Total Bases1.6 Mbp
Sequences flagged as poor quality0
Sequence length142-145
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTGTGTGTGTTTCTGTGGGTTTCTTTAAGGTTTGGACAGAAGGGTAAAG496342.61914984972091No Hit
ACTTAAAAACAAGGCAGTGCTAATGCTAATGGTGCTACAGTTTCTGCCTC403234.62430227565479No Hit
ACTTAAAAACAAGGCAGCTCTGAACGGTATTTCCTCTAAGACCAAGTCTC5915.075139544869042No Hit
ACTTAAAAACAAGGCAGTTCTGAACGGTATTTCCTCTAAGACCAAGTCTC1461.253756977243452No Hit
ACTTAAAACAAGGCAGTGCTAATGCTAATGGTGCTACAGTTTCTGCCTCT1391.193645341348218No Hit
ACTTAAAAACAAGGCAGTGCTGTGCCCTCCCAGGTACCCCAAGCTGGCAG1130.970373550880206No Hit
ACTTAAAAACAAGGCAGTGCTATGCCCTCCCAGGTACCCCAAGCTGGCAG780.6698153714040361No Hit
ACTTAAAAACAAGGCAGTGCTGAACGGTATTTCCTCTAAGACCAAGTCTC750.6440532417346501No Hit
CTTAAAAACAAGGCAGTGCTAATGCTAATGGTGCTACAGTTTCTGCCTCT460.39501932159725206No Hit
ACTTAAAAACAGGCAGTGCTAATGCTAATGGTGCTACAGTTTCTGCCTCT440.377844568484328No Hit
TGTGTGTGTGTTCTGTGGGTTTCTTTAAGGTTTGGACAGAAGGGTAAAGC330.28338342636324604No Hit
ACTTAAAAACAAGGCAGTGCTATCAACGAAGAGGTCCATTCTAGTGCCCC260.22327179046801202No Hit
TGTGTGTGTGTTTCTGTGGCAGCTCTGAACGGTATTTCCTCTAAGACCAA240.20609703735508805No Hit
TGTGTGTGTGTTTCTGTGGGTTTCTTTAAGGTTTGGACAGAAGGGTAAGG210.180334907685702No Hit
ACTTAAAAACAAGCAGTGCTAATGCTAATGGTGCTACAGTTTCTGCCTCT200.17174753112924002No Hit
ACTAAAAACAAGGCAGTGCTAATGCTAATGGTGCTACAGTTTCTGCCTCT180.15457277801631603No Hit
TGTGTGTGTTTCTGTGGGTTTCTTTAAGGTTTGGACAGAAGGGTAAAGCT180.15457277801631603No Hit
ACTTAAAACAAGGCAGCTCTGAACGGTATTTCCTCTAAGACCAAGTCTCT170.145985401459854No Hit
ACTTAAAAACAAGGCAGTGCTAATGCTAATGGTGCTACAGTTCCTGCCTC170.145985401459854No Hit
ATTAAAAACAAGGCAGTGCTAATGCTAATGGTGCTACAGTTTCTGCCTCT170.145985401459854No Hit
ACTTAAAAACAAGGCAGTGCTAATGCTAATGGTGCCACAGTTTCTGCCTC160.137398024903392No Hit
TGTGTGTGTGTTTCTGTGGGTTTCTTTAAGGTTTGGGCAGAAGGGTAAAG160.137398024903392No Hit
ACTTAAAAAAAGGCAGTGCTAATGCTAATGGTGCTACAGTTTCTGCCTCT150.12881064834693No Hit
TGTGTGTGTGTTTCTGTGGGTTTCTTTAAGGTTTGGACAGGAGGGTAAAG130.11163589523400601No Hit
TGTGTGTGTGTTTCTGTGGGTTTCTTTAGGTTTGGACAGAAGGGTAAAGC130.11163589523400601No Hit
ACTTAAAAACAAGGCAGTGCTAATGCTAATGGTGCTACAGTCTCTGCCTC130.11163589523400601No Hit
ACTTAAAAACAAGGCAGTGCTAATGCTAATGGTGCTACGGTTTCTGCCTC130.11163589523400601No Hit
TGTGTGTGTGTTTCTGTGGGTTTCTTTAGGGTTTGGACAGAAGGGTAAAG130.11163589523400601No Hit
TGTGTGTGTGTTTCTGTGGGTTTCCTTAAGGTTTGGACAGAAGGGTAAAG130.11163589523400601No Hit
ACTTAAAAACAAGGCAGTGCTAATGCTAATGGTGCTACAGTTTCTGCCCC120.10304851867754403No Hit
TGTGTGGTGTTTCTGTGGGTTTCTTTAAGGTTTGGACAGAAGGGTAAAGC120.10304851867754403No Hit
ACTTAAAAACAAGGCAGTGCTAATGCTAATGGTGCTACAGTTTCTGTCTC120.10304851867754403No Hit
TTGTGTGTGTTTCTGTGGGTTTCTTTAAGGTTTGGACAGAAGGGTAAAGC120.10304851867754403No Hit
ACTTAAAAACAAGGAGTGCTAATGCTAATGGTGCTACAGTTTCTGCCTCT120.10304851867754403No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)