FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008152304

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008152304
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11645
Total Bases1.6 Mbp
Sequences flagged as poor quality0
Sequence length141-145
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAATACCGTTCAGAGCTGCCACCAAGAGCTTGCAAATTGCTGCTGGAACC368031.601545727780163No Hit
GGCACTAGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATAC293425.19536281665951No Hit
GGCACTAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATAC145512.49463288965221No Hit
AAATACCGTTCAGAGCTGCCTTGTTTTTAAGTTTACGCTGTAGAACCATG5504.7230571060541005No Hit
AAATACCGTTCAGAACTGCCTTGTTTTTAAGTTTACGCTGTAGAACCATG1421.219407471017604No Hit
AAATACCGTTCAGAGCTGCCAGCTTGGGGTACCTGGGAGGGCACAGCACT1050.9016745384285102No Hit
AAATACCGTTCAGAGCTGCCAGCTTGGGGTACCTGGGAGGGCATAGCACT760.6526406182911121No Hit
AAATACCGTTCAGCACTGCCTTGTTTTTAAGTTTACGCTGTAGAACCATG690.592528982395878No Hit
AATACCGTTCAGAGCTGCCACCAAGAGCTTGCAAATTGCTGCTGGAACCT660.5667668527264921No Hit
GGCACTAGATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA650.55817947617003No Hit
GCACTAGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA580.49806784027479606No Hit
GGCACTAGATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA340.291970802919708No Hit
AAATACGTTCAGAGCTGCCACCAAGAGCTTGCAAATTGCTGCTGGAACCT330.28338342636324604No Hit
GGCACTAGAATGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA320.274796049806784No Hit
AAATACCGTTCAGAGCTGCCCCCAAGAGCTTGCAAATTGCTGCTGGAACC280.240446543580936No Hit
AAATACCGTTCAGAGCTGCCACCAAGAGCTTGCAAATTGCTGCTGGACCC270.23185916702447404No Hit
AAATACCGTCAGAGCTGCCACCAAGAGCTTGCAAATTGCTGCTGGAACCT260.22327179046801202No Hit
GGCACTGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA240.20609703735508805No Hit
GGCACTAGAATGGACCTCTTCGTTGATAGCACTGCCTTGTTTTTAAGTTT240.20609703735508805No Hit
GGACTAGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA220.188922284242164No Hit
AAATACCGTTCAGAGCTGCCACAGAAACACACACACATTACGCTGTAGAA220.188922284242164No Hit
AAATACCGTTCAAGCTGCCACCAAGAGCTTGCAAATTGCTGCTGGAACCT210.180334907685702No Hit
GGCACTAAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA210.180334907685702No Hit
AAATACCGTTCAGAGCTGCCACCAAGAGCTTGCAAATTGATGCTGGAACC200.17174753112924002No Hit
GGCACTAGAAGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA200.17174753112924002No Hit
AAATACCGTTCAGAGCTGCCACCAAGGGCTTGCAAATTGCTGCTGGAACC190.16316015457277802No Hit
GCACTAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA180.15457277801631603No Hit
GGCACTAGAATGGCCCTCTTCCTCATCACTAAATTATTTCAATTAAATAC170.145985401459854No Hit
AAATACCGTTCAGAGCTGCCTTGTTTTAAGTTTACGCTGTAGAACCATGT160.137398024903392No Hit
GGCACAGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA160.137398024903392No Hit
AAATACCTTCAGAGCTGCCACCAAGAGCTTGCAAATTGCTGCTGGAACCT160.137398024903392No Hit
GGCACTGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA160.137398024903392No Hit
AAATCCGTTCAGAGCTGCCACCAAGAGCTTGCAAATTGCTGCTGGAACCT150.12881064834693No Hit
GGCACTAGAATGGACTCTTCCTCATCACTAAATTATTTCAATTAAATACA150.12881064834693No Hit
GGCACTAGAATGGACCCTTCCTCATCACTAAATTATTTCAATTAAATACA150.12881064834693No Hit
AAATACCGTTAGAGCTGCCACCAAGAGCTTGCAAATTGCTGCTGGAACCT140.120223271790468No Hit
AAATACCGTTCAGAGCTGCCACCAAGCGCTTGCAAATTGCTGCTGGAACC130.11163589523400601No Hit
AAATACCGTTCAGAGCTGCCACCAAGAGCTTGCAAATCGCTGCTGGAACC130.11163589523400601No Hit
AAATACCGTTCAGAGCGCCACCAAGAGCTTGCAAATTGCTGCTGGAACCT130.11163589523400601No Hit
AAATACCGTTCAGAGCTGCCGCCAAGAGCTTGCAAATTGCTGCTGGAACC120.10304851867754403No Hit
AAATACCGTTCAGAGCTGCCACCAAGAGCTTGCAAATTGCCGCTGGAACC120.10304851867754403No Hit
GGCCTAGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA120.10304851867754403No Hit
AAATACCGTTCAGAGCTGCCACCAAGAGCCTGCAAATTGCTGCTGGAACC120.10304851867754403No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)