FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008152318

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008152318
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11385
Total Bases1.6 Mbp
Sequences flagged as poor quality0
Sequence length141-145
%GC36

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCACTAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATAC400635.18664909969258No Hit
AAATACCGTTCAGAGCTGCCACCAAGAGCTTGCAAATTGCTGCTGGAACC218019.148001756697408No Hit
GGCACTAGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATAC198817.46157224418094No Hit
AAATACCGTTCAGAGCTGCCTTGTTTTTAAGTTTACGCTGTAGAACCATG4273.7505489679402726No Hit
AAATACCGTTCAGAACTGCCTTGTTTTTAAGTTTACGCTGTAGAACCATG1201.0540184453227932No Hit
GGCACTAGATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA1030.9046991655687308No Hit
GCACTAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA880.7729468599033816No Hit
GCACTAGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA570.5006587615283268No Hit
GGCACTAGATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA560.4918752744839701No Hit
GGACTAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA530.46552481335090035No Hit
AAATACCGTTCAGCACTGCCTTGTTTTTAAGTTTACGCTGTAGAACCATG500.4391743522178305No Hit
GGCACTAAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA460.40404040404040403No Hit
GGCACTAGAATGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA420.3689064558629776No Hit
GGCACTGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA360.31620553359683795No Hit
GGCACAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA350.30742204655248134No Hit
AATACCGTTCAGAGCTGCCACCAAGAGCTTGCAAATTGCTGCTGGAACCT290.25472112428634164No Hit
GGCACTAGAATGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA280.24593763724198506No Hit
GGCATAGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA280.24593763724198506No Hit
GGCATAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA280.24593763724198506No Hit
GGCACTAAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA270.23715415019762848No Hit
GGCACTGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA270.23715415019762848No Hit
GGCACTAGAAGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA260.22837066315327184No Hit
GGACTAGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA250.21958717610891526No Hit
GGCCTAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA230.20202020202020202No Hit
GGCACTAGAATGGACTCTTCCTCATCACTACATTATTTCAATTAAATACA210.1844532279314888No Hit
GGCACTAGAAGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA200.1756697408871322No Hit
GGCACAGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA190.16688625384277558No Hit
AAATACGTTCAGAGCTGCCACCAAGAGCTTGCAAATTGCTGCTGGAACCT190.16688625384277558No Hit
GGCACTAGAATGGACCTCTTCGTTGATAGCACTGCCTTGTTTTTAAGTTT190.16688625384277558No Hit
GGCACTAGAATGGACCCTTCCTCATCACTACATTATTTCAATTAAATACA170.14931927975406237No Hit
GGCACTAGAATGGCCTCTTCCTCATCACTACATTATTTCAATTAAATACA170.14931927975406237No Hit
AAATACCGTTCAAGCTGCCACCAAGAGCTTGCAAATTGCTGCTGGAACCT150.13175230566534915No Hit
GGCACTAGAATGGCCCTCTTCCTCATCACTACATTATTTCAATTAAATAC140.12296881862099253No Hit
AAATACCGTTCAGAGCTGCCACCAAGAGCTTGCAAATTGCTGCTGGACCC140.12296881862099253No Hit
GGCCTAGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA130.11418533157663592No Hit
GGCACTAGAATGGACCCTTCCTCATCACTAAATTATTTCAATTAAATACA130.11418533157663592No Hit
AAATACCGTTCAGAGCTGCCACCAAGAGCTTGCAAATTGCTGTTGGAACC130.11418533157663592No Hit
AAATACCGTTAGAGCTGCCACCAAGAGCTTGCAAATTGCTGCTGGAACCT120.10540184453227931No Hit
AAATACCGTTCGAGCTGCCACCAAGAGCTTGCAAATTGCTGCTGGAACCT120.10540184453227931No Hit
AAATACCGTTCAGAGCTGCCAGCTTGGGGTACCTGGGAGGGCACAGCACT120.10540184453227931No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)