FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008152369

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008152369
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences612
Total Bases88.7 kbp
Sequences flagged as poor quality0
Sequence length141-145
%GC57

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACTTAAAAACAAGGCAGCTCTGAACGGTATTTCCTCTAAGACCAAGTCTC27344.6078431372549No Hit
ACTTAAAAACAAGGCAGTTCTGAACGGTATTTCCTCTAAGACCAAGTCTC11418.627450980392158No Hit
ACTTAAAAACAAGGCAGTGCTATCAACGAAGAGGTCCATTCTAGTGCCCC376.045751633986928No Hit
ACTTAAAAACAAGGCAGTGCTGAACGGTATTTCCTCTAAGACCAAGTCTC345.555555555555555No Hit
ACTTAAAACAAGGCAGCTCTGAACGGTATTTCCTCTAAGACCAAGTCTCT101.6339869281045754No Hit
ACTTAAAAACAAGGCAGTGGTATCAACGCAGAGGTCCATTCTAGTGCCCC101.6339869281045754Clontech Universal Primer Mix Long (95% over 22bp)
ACTTAAAAACAAGGCAGTGGTATCAACGAAGAGGTCCATTCTAGTGCCCC101.6339869281045754No Hit
TGTGTGTGTGTTTCTGTGGCAGCTCTGAACGGTATTTCCTCTAAGACCAA71.1437908496732025No Hit
ACTTAAAAACAAGGCAGTCTGAACGGTATTTCCTCTAAGACCAAGTCTCT71.1437908496732025No Hit
CTTAAAAACAAGGCAGCTCTGAACGGTATTTCCTCTAAGACCAAGTCTCT60.9803921568627451No Hit
ACTTAAAAACAAGGCAGCTCTGAACGGTATTCCTCTAAGACCAAGTCTCT60.9803921568627451No Hit
ACTTAAAAACAAAGCAGTGGTATCAACGAAGAGGTCCATTCTAGTGCCCC40.6535947712418301Clontech Universal Primer Mix Long (95% over 22bp)
TGTGTGTGTGTTTCTGTGGGTACTGAGATGTTTCACTTCCCCACGTTCCC40.6535947712418301No Hit
ACTTAAAAACAAGGCAGCTTGAACGGTATTTCCTCTAAGACCAAGTCTCT30.49019607843137253No Hit
ACTTAAAAACAAGGCAGTGCTATCAACGCAGAGGTCCATTCTAGTGCCCC30.49019607843137253No Hit
ACTTAAAAACAAAGCAGTGGTATCAACGCAGAGGTCCATTCTAGTGCCCC30.49019607843137253Clontech Universal Primer Mix Long (95% over 22bp)
ACTTAAAAACAAGGCAGCTCTGAACGTATTTCCTCTAAGACCAAGTCTCT20.32679738562091504No Hit
ACTTAAAAAAAGGCAGCTCTGAACGGTATTTCCTCTAAGACCAAGTCTCT20.32679738562091504No Hit
ACTTAAAAACAAGGCAGTGCTGAACGGTATTTCCTCTAAGCCCAAGTCTC20.32679738562091504No Hit
TGTGTGTGTGTTTCTGTGGGTAAGCAGTGGTATCAACGAAGAGGTCCATT20.32679738562091504Clontech SMARTer II A Oligonucleotide (95% over 22bp)
ATTAAAAACAAGGCAGCTCTGAACGGTATTTCCTCTAAGACCAAGTCTCT20.32679738562091504No Hit
ACTTAAAAACAAGGCAGTGCTGAACGTATTTCCTCTAAGACCAAGTCTCT10.16339869281045752No Hit
TGTGTGTGTGTTTCTGTGGGTCTTGGTCGAAGAGGTCCATTCTAGTGCCC10.16339869281045752No Hit
ACTTAAAAACAAGGCATGCTATCAACGAAGAGGTCCATTCTAGTGCCCCT10.16339869281045752No Hit
ACTTAAAAACAAGGCAGTTCTGAACTGTATTTCCTCTAAGACCAAGTCTC10.16339869281045752No Hit
ACTTAAAAACAAAGCAGTGGTATCAACGCAGAGTTCCATTCTAGTGCCCC10.16339869281045752Clontech SMARTer II A Oligonucleotide (96% over 25bp)
ACTTAAAAACAAGGCAGCTCTAAACGGTATTTCCTCTAAGACCAAGTCTC10.16339869281045752No Hit
ACTTAAAAACAAGGCAGCTCTGAACGGTATTTACTCTAAGACCAAGTCTA10.16339869281045752No Hit
ACTTAAAAACAGGCAGCTCTGAACGGTATTTCCTCTAAGACCAAGTCTCT10.16339869281045752No Hit
ACTTAAAAACAAGGCAGTGCTATCAACGAAGAGGTCCATTCTAGTCCCCT10.16339869281045752No Hit
ACTTTAAAACAAGGCAGCTCTGAACGGTATTTCCTCTAAGACCAAGTCTC10.16339869281045752No Hit
TGTGTGTGTGTTTCTGTGGCAGATCTGAACGGTATTTCCTCTAAGACCAA10.16339869281045752No Hit
ACTTAAAAACAAGGCAGCTCTAACGGTATTTCCTCTAAGACCAAGTCTCT10.16339869281045752No Hit
ACTTAAAAACAAGGCAGTGCTATCAACGAAGAGGTCATTCTAGTGCCCCT10.16339869281045752No Hit
ACTTAAAAACAAGGCAGTGCTGACGGTATTTCCTCTAAGACCAAGTCTCT10.16339869281045752No Hit
ACTTAAAAACAAGGCAGCTCTGAACGGTATATCCTCTAAGACCAAGTCTC10.16339869281045752No Hit
ACTTAAAAACAAGGCAGTTCTGAACGGTACTTCCTCTAAGACCAAGTCTC10.16339869281045752No Hit
ACTTAAAAACAAGGCAGTGCTATCAACGCAGAGTTGCTGGCGAGTACCTC10.16339869281045752No Hit
ACTTAAAAACAAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGAGGT10.16339869281045752Clontech SMARTer II A Oligonucleotide (100% over 25bp)
ACTTAAAACAAGGCAGTGCTGAACGGTATTTCCTCTAAGACCAAGTCTCT10.16339869281045752No Hit
ACTTAAAAACAAGGCAGTGCTATCAACCGAAGAGGTCCATTCTAGTGCCC10.16339869281045752No Hit
ACTTAAAAAAAAGGCAGTTCTGAACGGTATTTCCTCTAAGACCAAGTCTC10.16339869281045752No Hit
ACTTAAAAACAAGGCACTCTGAACGGTATTTCCTCTAAGACCAAGTCTCT10.16339869281045752No Hit
ACTTAAAACAAGGCAGCTTGAACGGTATTTCCTCTAAGACCAAGTCTCTG10.16339869281045752No Hit
CTTAAAACAAGGCAGTTCTGAACGGTATTTCCTCTAAGACCAAGTCTCTG10.16339869281045752No Hit
ACTTAAAAACAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGT10.16339869281045752Clontech SMARTer II A Oligonucleotide (100% over 25bp)
TGTGTGTGTGTTTCTGTGGGTGTCTGGCAGCTCTGAACGGTATTTCCTCT10.16339869281045752No Hit
ACTTAAAAACAAGGCAGTTCTGAACGGTATTTCATCTAAGACCAAGTCTC10.16339869281045752No Hit
CTTAAAAACAAGGCAGTTCTGAACGGTATTTCCTCTAAGACCAAGTCTCT10.16339869281045752No Hit
ACTTAAAAACAAGCAGTGGTATCAACGCAGAGTACATGGCAGCAGTGGTA10.16339869281045752Clontech SMARTer II A Oligonucleotide (100% over 25bp)
ACTTAAAAACAAGGCAGCTCTGACGGTATTTCCTCTAAGACCAAGTCTCT10.16339869281045752No Hit
ACTTAAAAACAAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG10.16339869281045752Clontech SMARTer II A Oligonucleotide (100% over 25bp)
ACTTAAAAACAAGGCAGTGCTCCGTAGGCAGCTCTGAACGGTATTTCCTC10.16339869281045752No Hit
TGTGTGTGTGTTTCTGTGGGTTTGGTCGAAGAGGTCCATTCTAGTGCCCC10.16339869281045752No Hit
ACTTAAAAACAAAGCAGTGGTATCAACGCAGAGTACATGAGGCAGTGGTA10.16339869281045752Clontech SMARTer II A Oligonucleotide (100% over 25bp)
ACTTAAAAACAAGGAAGTGCTGAACGGTATTTCCTCTAAGACCAAGTCTC10.16339869281045752No Hit
ACTTAAAAACAAGGTAGCTCTGAACGGTATTTCCTCTAAGACCAAGTCTC10.16339869281045752No Hit
ACTTAAAAACAAGGCAATGCTATCAACGAAGAGGTCCATTCTAGTGCCCC10.16339869281045752No Hit
ACTAAAAACAAGGCAGTGCTGAACGGTATTTCCTCTAAGACCAAGTCTCT10.16339869281045752No Hit
TGTGTGTGTGTTTCTGGCAGCTCTGAACGGTATTTCCTCTAAGACCAAGT10.16339869281045752No Hit
ACTAAAAACAAGGCAGTGCTATCAACGAAGAGGTCCATTCTAGTGCCCCT10.16339869281045752No Hit
ACTTAAAAACAAGGCAGTGCTACCGTAGGCAGCTCTGAACGGTATTTCCT10.16339869281045752No Hit
ACTTAAAAACAAGGCAGCTCTGAACGGTATCCTCTAAGACCAAGTCTCTG10.16339869281045752No Hit
ACTTAAAAACAAGGCAGTTTGAACGGTATTTCCTCTAAGACCAAGTCTCT10.16339869281045752No Hit
ACTTAAACAAGGCAGTTCTGAACGGTATTTCCTCTAAGACCAAGTCTCTG10.16339869281045752No Hit
TGTGTGTGTGTTTCTGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTA10.16339869281045752No Hit
ATTAAAAACAAGGCAGTTCTGAACGGTATTTCCTCTAAGACCAAGTCTCT10.16339869281045752No Hit
ACTTAAAAACAAGGCAGTGATATCAACGAAGAGGTCCATTCTAGTGCCAC10.16339869281045752No Hit
ACTTAAAAACAAGGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGT10.16339869281045752Clontech SMARTer II A Oligonucleotide (96% over 25bp)
ACTTAAAAACAAGGCAGTGCTAACAACGAAGAGGTCCATTCTAGTGCCCC10.16339869281045752No Hit
ACTTAAAAACAAGGAAGTTCTGAACGGTATTTCCTCTAAGACCAACTCTC10.16339869281045752No Hit
ACTTAAAAACAAGGCAGTTCTGAACGGTATTCCCTCTAAGACCAAGTCTC10.16339869281045752No Hit
TGTGTGTGTGTTTCTGTGGGAAGCAGTGGTATCAACGCAGAGGTCCATTC10.16339869281045752Clontech Universal Primer Mix Long (96% over 25bp)
ACTTAAAAACAAGGCAGCTCTGAACGGTATTTCCTCTAAGACAAACTCTC10.16339869281045752No Hit
TGTGTGTGTGTTTCTGTGGGTCCATTCTAGTGCCCCTCTAAGACCAAGTC10.16339869281045752No Hit
ACTTAAAAACATGGCAGCTCTGAACGGTATTTCCTCTAAGACCAAGTCTC10.16339869281045752No Hit
ACTTAAAAACAAGGCCGCTCTGACGGTATTTCCTCTAAGACCAAGTCTCT10.16339869281045752No Hit
ACTTAAAAACAAAGCAGTGGTATCAACGCAGAGTACATGGGAGCAGTGGT10.16339869281045752Clontech SMARTer II A Oligonucleotide (100% over 25bp)
TGTGTGTGTGTTTCTGTGGTATCAACGCAGAGTACATGGGAGCAGTGGTA10.16339869281045752No Hit
ACTTAAAAACAAGGAAGTGGTATCAACGCAGAGGTCCATTCTAGTGCCCC10.16339869281045752No Hit
ACTTAAAAACAAGGCAGTTCTGATCGGTATTTCCTCTAAGACCAAGTCTC10.16339869281045752No Hit
ACTTAAAAACAAGGCAGTGGTATCAACGAAGAGGCCATTCTAGTGCCCCT10.16339869281045752No Hit
ACTTAAAAACAAGGCAGTGGTATCAACGAAGAGGTCCATTCTAGTGCCCT10.16339869281045752No Hit
ACTTAAAAACAAGGAGTGCTATCAACGAAGAGGTCCATTCTAGTGCCCCT10.16339869281045752No Hit
ACTTAAAAACAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTA10.16339869281045752Clontech SMARTer II A Oligonucleotide (100% over 25bp)
ACTTAAAAACAAGGCAGTGCTATCAACGCAGAGTACATGGAAGCAGTGGT10.16339869281045752No Hit
ACTTAAAACAAGGCAGTGCTATCAACGAAAGGTCCATTCTAGTGCCCCTC10.16339869281045752No Hit
CTTAAAAACAAGCAGTGGTATCAACGCAGAGGTCCATTCTAGTGCCCCTC10.16339869281045752Clontech Universal Primer Mix Long (95% over 24bp)
ACTTAAAAACAAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGT10.16339869281045752Clontech SMARTer II A Oligonucleotide (100% over 25bp)
ACTTAAAAACAAGCAGTGGTATCAACGCAGAGTACATGGGGTAGTGGTGG10.16339869281045752Clontech SMARTer II A Oligonucleotide (100% over 25bp)
ACTTAAAAACAAGGCAGTGCTATCAACGAAGAGGTACATTCTAGTGCCCC10.16339869281045752No Hit
TGTGTGTGTGTTTCTGTGGGTGTCAGTGTAGAAGAGGTCCATTCTAGTGC10.16339869281045752No Hit

[FAIL]Adapter Content

Adapter graph