FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008152370

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008152370
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences612
Total Bases88.7 kbp
Sequences flagged as poor quality0
Sequence length144-145
%GC59

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAATACCGTTCAGAGCTGCCTTGTTTTTAAGTTTACGCTGTAGAACCATG26242.810457516339866No Hit
AAATACCGTTCAGAACTGCCTTGTTTTTAAGTTTACGCTGTAGAACCATG11318.4640522875817No Hit
GGCACTAGAATGGACCTCTTCGTTGATAGCACTGCCTTGTTTTTAAGTTT376.045751633986928No Hit
AAATACCGTTCAGCACTGCCTTGTTTTTAAGTTTACGCTGTAGAACCATG355.718954248366013No Hit
GGCACTAGAATGGACCTCTTCGTTGATACCACTGCCTTGTTTTTAAGTTT101.6339869281045754No Hit
AAATACCGTTCAGAGCTGCCTTGTTTTAAGTTTACGCTGTAGAACCATGT101.6339869281045754No Hit
GGCACTAGAATGGACCTCTGCGTTGATACCACTGCCTTGTTTTTAAGTTT91.4705882352941175Clontech SMARTer II A Oligonucleotide (95% over 22bp)
AAATACCGTTCAGACTGCCTTGTTTTTAAGTTTACGCTGTAGAACCATGT71.1437908496732025No Hit
AAATACCGTTCAGAGCTGCCTTGTTTTTAAGTTACGCTGTAGAACCATGT60.9803921568627451No Hit
AAATACCGTTCAGAGCTGCCACAGAAACACACACACATTACGCTGTAGAA60.9803921568627451No Hit
AATACCGTTCAGAGCTGCCTTGTTTTTAAGTTTACGCTGTAGAACCATGT50.8169934640522877No Hit
GGCACTAGAATGGACCTCTGCGTTGATACCACTGCTTCCATGTACTCTGC40.6535947712418301Clontech Universal Primer Mix Long (95% over 23bp)
AAATACCGTTCAAGCTGCCTTGTTTTTAAGTTTACGCTGTAGAACCATGT30.49019607843137253No Hit
GGCACTAGAATGGACCTCTTCGTTGATACCACTGCTTTGTTTTTAAGTTT30.49019607843137253Clontech SMARTer II A Oligonucleotide (95% over 22bp)
AAATACCGTTCAGAGCTGCCAATCGAGCTTTGATCCGAGGATTTCCGAAT30.49019607843137253No Hit
GGCACTAGAATGGACCTCTGCGTTGATAGCACTGCCTTGTTTTTAAGTTT30.49019607843137253No Hit
AAATACCGTTCAGAGCTGCCTTTTTTTAAGTTTACGCTGTAGAACCATGT20.32679738562091504No Hit
GGCACTAGAATGGACCTCTGCGTTGATACCACTGCTTTGTTTTTAAGTTT20.32679738562091504Clontech Universal Primer Mix Long (95% over 23bp)
GGCACTAGAATGGACCTCTTCGTTGATACCACTGCTTACCCACAGAAACA20.32679738562091504Clontech SMARTer II A Oligonucleotide (95% over 22bp)
AAATACCGTTCAGAGCTGCCTTGTTTTTAAGTTTACGCCGTAGAACCATG20.32679738562091504No Hit
AAATACGTTCAGAGCTGCCTTGTTTTTAAGTTTACGCTGTAGAACCATGT20.32679738562091504No Hit
AAATACCGTTCAGAGCTGCCTTGTTTTTAATTTACGCTGTAGAACCATGT20.32679738562091504No Hit
GGCACTAGAATGGACCTCTTCGTTGATACCACTGCTCCATGTACTCTGCG10.16339869281045752Clontech SMARTer II A Oligonucleotide (95% over 21bp)
AAATACCGTTCAGAGCTGCCTTGTTTTTAAGTTTACGCTGTAGCACCATG10.16339869281045752No Hit
AAATACCGTTCAGAGCTGCCAGACACCCACAGAAACACACACACATTACG10.16339869281045752No Hit
GGCACTAGAATGGACCTTTCGTTGATAGCACTGCCTTGTTTTAAGTTTAC10.16339869281045752No Hit
AAATACCGTTCAGAACTGCCTTGTTTTTAAGTTACGCTGTAGAACCATGT10.16339869281045752No Hit
GGCACTAGAATGGACCTCTTCTTCAGCCACCACTACCCCATGTACTCTGC10.16339869281045752No Hit
GGCACTAGAATGGACCTCTGCGTTGATACCACTGGTTTGTTTTTAAGTTT10.16339869281045752Clontech SMARTer II A Oligonucleotide (95% over 22bp)
AAATACCGTTCAGCACTGCCTTGTTTTTAAGTTTACGCTGTAGCACCATG10.16339869281045752No Hit
AAATACCGTCAGCACTGCCTTGTTTTTAAGTTTACGCTGTAGAACCATGT10.16339869281045752No Hit
AAATACCGTTCAGAGCTGCCTTGTTTTTAAGTTTACGCTGTAGACCCATG10.16339869281045752No Hit
AAATACCGTTCAAGCTGCCTTGTTTTAAGTTTACGCTGTAGAACCATGTC10.16339869281045752No Hit
GGCACTGGAATGGACCTCTTCGTTGATACCACTGCTTCCCATGTACTCTG10.16339869281045752Clontech SMARTer II A Oligonucleotide (95% over 22bp)
AAATACCGTTTAGAGCTGCCTTGTTTTTAAGTTTACGCTGTAGAACCATG10.16339869281045752No Hit
GGCACTAGAATGGACCTCTGCGTTGATACCACTGCTTGTTTTTAAGTTAC10.16339869281045752Clontech Universal Primer Mix Long (95% over 24bp)
ATATACCGTTCAGAGCTGCCTTGTTTTTAAGTTTACGCTGTAGAACCATG10.16339869281045752No Hit
GGCACTAGAATGGACCTCTTCGTTGATAGCACTCCTTGTTTTTAAGTTTA10.16339869281045752No Hit
AAATACCGTCAGAGCTGCCTTGTTTGTAAGTTTACGCTGTAGAACCATGT10.16339869281045752No Hit
GGCACTAGAATGGACCTCTACGTTGATAGCACTGCCATGTTTTTAAGTTT10.16339869281045752No Hit
AAATACCGTTCAGACCTGCCTTGTTTTTAAGTTTACGCTGTAGAACAATG10.16339869281045752No Hit
AAGTACCGTTCAGAACTGCCTTGTTTTTAAGTTTACGCTGTAGAACCATG10.16339869281045752No Hit
GGCACTAGAATGGACCCACAGAAACACACACACATTACGCTGTAGAACCA10.16339869281045752No Hit
AAATACCGTTCAGAACTGCCTTGTTTTAAGTTACGCTGTAGAACCATGTC10.16339869281045752No Hit
GGCACTAGAATGGACCTCTTCGTTGATACCACTGCCCCATGTACTCTGCG10.16339869281045752No Hit
GGCACTAGAATGGACCTCTTCGTTGATAGCATGCCTTGTTTTTAAGTTTA10.16339869281045752No Hit
AAATACCGTTCAGAGCAGCCTAGTTTTTAAGTTTACGCTGAATAACCATG10.16339869281045752No Hit
AAATACCGTTCAGAACTGACTTGTTTTTAAGTTTACGCTGTAGAAACATG10.16339869281045752No Hit
AAATACGTTCAGCACTGCCTTGTTTTTAAGTTTACGCTGTAGAACCATGT10.16339869281045752No Hit
AAATACCGTTCAGAGCTACCTTGTTTTTAAGTTTACGCTGTAGAACCATG10.16339869281045752No Hit
GGCACTAGAATGACCTCTTCGTTGATAGCACTGCCTTGTTTTTAAGTTTA10.16339869281045752No Hit
AAATACCGTTCAAACTGCCTTGTTTTTAAGTTTACGCTGTAGAACCATGT10.16339869281045752No Hit
AAATACCGTTCAGCACTGCCTTGTTTTTAGTTTACGCTGTAGAACCATGT10.16339869281045752No Hit
AAATACCGTTCAGAACTGCCTTGTTTTTAATTTACGCTGTAGAACCATGT10.16339869281045752No Hit
AAATACCGTTCAGAACTGCCTTGTTTTTAAGTTTACGCTGTAGACCCATG10.16339869281045752No Hit
AAATACCGTTCAGAGCTGCCTACGGTAGCACTGCCTTGTTTTTAAGTTTA10.16339869281045752No Hit
AAATACCGTTCAGAGCTGCCTTGTTTTTAAGTTTACGCTGTAGAACATGT10.16339869281045752No Hit
GGACTAGAATGGACCTCTTCGTTGATAGCACTGCCTTGTTTTTAAGTTTA10.16339869281045752No Hit
GGCACTAGAATGGACCTCTTCGTTGATACCACTGCTTCCCATGTACTCTG10.16339869281045752Clontech SMARTer II A Oligonucleotide (95% over 22bp)
GGCACTAGAATGGCCTCTTCGTTGATACCACTGCCTTGTTTTTAAGTTTA10.16339869281045752No Hit
GAATACCGTTCAGAACTGCCTTGTTTTTAAGTTTACGCTGTAGAACCATG10.16339869281045752No Hit
AAATACCGTTCAGAGTGCCTTGTTTTTAAGTTTACGCTGTAGAACCATGT10.16339869281045752No Hit
AAATACCGTTCAGAGCTGCCTTGTTTTTAAGTTTAAGCTGTAGAACCATG10.16339869281045752No Hit
GGCACTAGAATGGACCTCTGCGTTGATACCACTTCCTTGTTTTTAAGTTT10.16339869281045752No Hit
AAATACCGTTCAGAGCTGCCACAGAAACAAACACACATTACGCTGTAGAA10.16339869281045752No Hit
GCACTAGAATGGACCTCTTCGTTGATACCACTGCCTTGTTTTTAAGTTTA10.16339869281045752No Hit
GGCACTAGAATGGACCTCTTCTACACTGACACCCACAGAAACACACACAC10.16339869281045752No Hit
GGCACTAGAATGGACCTCTGCGTTGATACCACTGACTTGTTTTTAAGTTT10.16339869281045752No Hit
GGCACTAGAATGGACCTCTTAGATGATAGCACTGCCTTGTTTTTAAGTTT10.16339869281045752No Hit
AATACCGTTCAGAGCTGCCTTGTTTTTAAGTTTACGCTGTAGACCCATGT10.16339869281045752No Hit
AAATACCGTTCAGAGCTGCCTTGTTTTTAAGTTTACGCTGTAGAACTATG10.16339869281045752No Hit
AAATACCGTTCAGAGCTGCCTACGGAGCACTGCCTTGTTTTTAAGTTTAC10.16339869281045752No Hit
AAATACGTTCAGAGCTGCCAATCGAGCTTTGATCCGAGGATTTCCGAATG10.16339869281045752No Hit
AAATACCGTTCAGCACTGCCTTGTTTTAAGTTTACGCTGTAGAACCATGT10.16339869281045752No Hit
AAATACCGTTCAGAGCTGCCTTGTTTTTAAGTTTACGCTGTAGAAACATG10.16339869281045752No Hit
GGCACTAGAATGGACCTCTTCGACCAAACCCACAGAAACACACACACATT10.16339869281045752No Hit
GGCACTAGAATGGACCTCTTCGTTGATAGCACTGCCTTGTTTTTAGTTTA10.16339869281045752No Hit
AAATACCGTTCAGAGCTGCCACAGAAAAAAAAAAAAAAAAAAAAAAAAAA10.16339869281045752No Hit
AAATACCGTCAGAGCTGCCTTGTTTTTAAGTTTACGCTGTAGAACCATGT10.16339869281045752No Hit
GGCACTAGAATGGACCTCTTCGTTGATACCACTGCTTCCATGTACTCTGC10.16339869281045752Clontech SMARTer II A Oligonucleotide (95% over 22bp)
GGCACTAGAATGGACCTCTTCGTTGATACCACTGCTTTGTTATTAAGTTT10.16339869281045752Clontech SMARTer II A Oligonucleotide (95% over 22bp)
GGCACTAGAATGGACCTCTTCGTTGTTAGCACTGCCTTGTTTTTAAGTTT10.16339869281045752No Hit
ATACCGTTCAGAGCTGCCTTGTTTTTAAGTTTACGCTGTAGAACCATGTC10.16339869281045752No Hit
GGCACTAGAATGGACCTCTGCGTTGATACCACTGCTTCCCACAGAAACAC10.16339869281045752Clontech Universal Primer Mix Long (95% over 23bp)
GGCACTAGAATGGACCTCTTCGTTGATAGCATTGCCTTGTTTTTAAGTTT10.16339869281045752No Hit
AAATACCGTTCAGAACTGCCTTTTTTTTAAGTTTACGCTGTAGAACCATG10.16339869281045752No Hit
AAATACCGATCAGAACTGCCTTGTTTTTAAGTTTACGCTGTAGAACCATG10.16339869281045752No Hit
AAATACCGTTCAGAACTGCCTTGTTTTTAAGTTTACGCTGTAGCACCATG10.16339869281045752No Hit
AAATACCGTTCAGAGCTGCCATGTTTTTAAGTTTACGCTGTAGAACCATG10.16339869281045752No Hit
AAATACCGTTCAGCACTTCCTTGTTTTTAAGTTTACGCTGTAGAACCATG10.16339869281045752No Hit
AAATACCGTTAGAGCTGCCTTGTTTTTAAGTTTACGCTGTAGAACCATGT10.16339869281045752No Hit
AAATACCGTTCAGAGCTGCCTTGTTTTTAAGTTTACGCTGTGGAACCATG10.16339869281045752No Hit
AAATACCGTTCAGAGCTGCCTGTTTTTAAGTTTACGCTGTAGAACCATGT10.16339869281045752No Hit
GGCACTAGAATGGACCTCTTCGGTTGATAGCACTGCCTTGTTTTTAAGTT10.16339869281045752No Hit
AAATCCCGTTCAGAACTGCCTTGTTTTTAAGTTTACGCTGTAGAACCATG10.16339869281045752No Hit
GGCACTAGAATGGACCTCTTCGTTGATACCACAGCTCCCAAGTACACTCC10.16339869281045752No Hit
AAATACCGGTCCGAGCTGCCACAGAAACACCCACACCGTACGCTTTATCA10.16339869281045752No Hit
AAATACCGTTCAGAGCTGCCTTGTTTTTAAGTTTACTCTGTAGAACAATG10.16339869281045752No Hit
AAATACCGTTCAGAGCTGCCAGAAACACACACACATTACGCTGTAGAACC10.16339869281045752No Hit
GGCACTAGAATGGAACTCTGCGTTGATACCACTGCTTTGTTTTTAAGTTT10.16339869281045752Clontech SMARTer II A Oligonucleotide (96% over 25bp)
AAATACCGTTCAGAACTGCCTTGTTTAAGTTTACGCTGTAGAACCATGTC10.16339869281045752No Hit
AAATACCGTTCGGAGCTGCCTTGTTTTTAAGTTTACGCTGTAGAACCATG10.16339869281045752No Hit
GGCACTAGAATGGACCTCTTCGACCAAGACCCACAGAAACACACACACAT10.16339869281045752No Hit
AAATACCGTTCAGAGCTGCCTTGTTTTAAAGTTTACGCTGTAGAACCATG10.16339869281045752No Hit
GGCACTAGAATGGACCTCTTCGTTGATACCACTGCCTCATGTACTCTGCG10.16339869281045752No Hit
GGCACTAGAATGGACCTCTTCGTTGATACCACTGCTCCCATGTACTCTGC10.16339869281045752Clontech SMARTer II A Oligonucleotide (95% over 21bp)

[FAIL]Adapter Content

Adapter graph