FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008152374

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008152374
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8680
Total Bases1.2 Mbp
Sequences flagged as poor quality0
Sequence length142-145
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCACTAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATAC259329.87327188940092No Hit
AAATACCGTTCAGAGCTGCCTTGTTTTTAAGTGGCTAATGTAGAACCATG148617.119815668202765No Hit
GGCACTAGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATAC100211.543778801843319No Hit
AAATACCGTTCAGAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGAACC6016.9239631336405525No Hit
AAATACCGTTCAGAGCTGCCACCAAGAGCTTGCAAATTGCTGCTGGAACC5386.1981566820276495No Hit
AAATACCGTTCAGAACTGCCTTGTTTTTAAGTGGCTAATGTAGAACCATG3574.112903225806452No Hit
AAATACCGTTCAGCACTGCCTTGTTTTTAAGTGGCTAATGTAGAACCATG1131.3018433179723503No Hit
AAATACCGTTCAGAGCTGCCAGCTTGGGGTACCTGGGAGGGCACAGCACT931.0714285714285714No Hit
AAATACCGTTCAGAGCTGCCAGCTTGGGGTACCTGGGAGGGCATAGCACT570.6566820276497696No Hit
GGCACTAGATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA440.5069124423963134No Hit
GGCACTAGAATGGACCTCTTCGTTGATAGCACTGCCTTGTTTTTAAGTGG400.4608294930875576No Hit
GGCACTAAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA340.391705069124424No Hit
AAATACCGTTCAGAGCTGCCTTGTTTTAAGTGGCTAATGTAGAACCATGT290.33410138248847926No Hit
GCACTAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA280.3225806451612903No Hit
AATACCGTTCAGAGCTGCCTTGTTTTTAAGTGGCTAATGTAGAACCATGT270.31105990783410137No Hit
GGCATAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA250.28801843317972353No Hit
AATACCGTTCAGAGCTGCCACCAAGAGCTTGCAAATTGCTGCTGGAACCT220.2534562211981567No Hit
GGCACTGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA200.2304147465437788No Hit
AATACCGTTCAGAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGAACCT190.21889400921658986No Hit
GGCACTAGAATGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA180.20737327188940094No Hit
GGACTAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA170.195852534562212No Hit
GGCACAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA170.195852534562212No Hit
GGCACTAGAAGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA130.14976958525345624No Hit
GGCACTAGAATGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA120.1382488479262673No Hit
GGCATAGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA120.1382488479262673No Hit
GGCCTAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA110.12672811059907835No Hit
AAATACGTTCAGAGCTGCCTTGTTTTTAAGTGGCTAATGTAGAACCATGT110.12672811059907835No Hit
GGCACTAGAATGGACCCTTCCTCATCACTACATTATTTCAATTAAATACA110.12672811059907835No Hit
AAATACCGTTAGAGCTGCCTTGTTTTTAAGTGGCTAATGTAGAACCATGT110.12672811059907835No Hit
GGCACTAGATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA110.12672811059907835No Hit
AAATACCGTCAGAGCTGCCTTGTTTTTAAGTGGCTAATGTAGAACCATGT110.12672811059907835No Hit
GGCACTAGAATGGACCTCTTCGTTGATACCACTGCCTTGTTTTTAAGTGG100.1152073732718894No Hit
GCACTAGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA100.1152073732718894No Hit
GGCACTAAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA100.1152073732718894No Hit
AAATACCGTTCAGAGCTGCCATTGCCCCACCCACTTCTCTGGTAGAGAAA100.1152073732718894No Hit
AAATACCGTTCAGAGCTGCCTTGTTTTTAGTGGCTAATGTAGAACCATGT100.1152073732718894No Hit
AAATACCGTTCAGAACTGCCTTGTTTTAAGTGGCTAATGTAGAACCATGT90.10368663594470047No Hit
GGACTAGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA90.10368663594470047No Hit
GGCACTGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA90.10368663594470047No Hit
GGCACTAGAATGGACCTCTTCTCATCACTACATTATTTCAATTAAATACA90.10368663594470047No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)