FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008152450

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008152450
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3316
Total Bases480.6 kbp
Sequences flagged as poor quality0
Sequence length141-145
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCACTAGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATAC49514.927623642943304No Hit
GGCACTAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATAC48214.535585042219543No Hit
AAATACCGTTCAGAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGAACC46814.11338962605549No Hit
AAATACCGTTCAGAGCTGCCTTGTTTTTAAGTGGCTAATGTAGAACCATG46413.99276236429433No Hit
AAATACCGTTCAGAGCTGCCACCAAGAGCTTGCAAATTGCTGCTGGAACC41312.454764776839566No Hit
AAATACCGTTCAGAACTGCCTTGTTTTTAAGTGGCTAATGTAGAACCATG962.8950542822677927No Hit
AAATACCGTTCAGAGCTGCCAGCTTGGGGTACCTGGGAGGGCACAGCACT531.5983112183353438No Hit
AAATACCGTTCAGAGCTGCCAGCTTGGGGTACCTGGGAGGGCATAGCACT300.9047044632086853No Hit
AAATACCGTTCAGCACTGCCTTGTTTTTAAGTGGCTAATGTAGAACCATG270.8142340168878166No Hit
GGCACTAGAATGGACCTCTTCGTTGATAGCACTGCCTTGTTTTTAAGTGG160.48250904704463204No Hit
AATACCGTTCAGAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGAACCT140.4221954161640531No Hit
GGCACTAGATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA110.33172496984318456No Hit
AAATACCGTTCAGAGCTGCCTTGTTTTAAGTGGCTAATGTAGAACCATGT110.33172496984318456No Hit
AATACCGTTCAGAGCTGCCTTGTTTTTAAGTGGCTAATGTAGAACCATGT110.33172496984318456No Hit
GGCACTAAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA100.30156815440289503No Hit
GGCACTAGATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA100.30156815440289503No Hit
GGCACTGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA100.30156815440289503No Hit
GGCACTGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA80.24125452352231602No Hit
AAATACGTTCAGAGCTGCCTTGTTTTTAAGTGGCTAATGTAGAACCATGT70.21109770808202655No Hit
GGCACTAGAATGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA70.21109770808202655No Hit
AAATACCGTTAGAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGAACCT60.18094089264173704No Hit
AAATACCGTTCAGAACTGCCTTGTTTTAAGTGGCTAATGTAGAACCATGT60.18094089264173704No Hit
AAATACCGTCAGAGCTGCCTTGTTTTTAAGTGGCTAATGTAGAACCATGT60.18094089264173704No Hit
GGCATAGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA60.18094089264173704No Hit
AATACCGTTCAGAGCTGCCACCAAGAGCTTGCAAATTGCTGCTGGAACCT60.18094089264173704No Hit
GGCATAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA60.18094089264173704No Hit
GCACTAGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA50.15078407720144751No Hit
AAATACCGTTCAGACTGCCTTGTTTTTAAGTGGCTAATGTAGAACCATGT50.15078407720144751No Hit
AAATACCGTTCAGAGCTGCCTTGTTTTTAATGGCTAATGTAGAACCATGT50.15078407720144751No Hit
GGCACTAGAATGGACTCTTCCTCATCACTACATTATTTCAATTAAATACA50.15078407720144751No Hit
AAATACCGTTCAGAGCTGCCCCCAAGAGCTTGCAAATTGCTGCTGGAACC40.12062726176115801No Hit
GGCACTAGAATGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA40.12062726176115801No Hit
GGCCTAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA40.12062726176115801No Hit
GGCACAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA40.12062726176115801No Hit
GGCACTAGAATGGACCTCTTCCCCATCACTAAATTATTTCAATTAAATAC40.12062726176115801No Hit
AAATACCGTTCGAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGAACCT40.12062726176115801No Hit
AAATACCGTTCGAGCTGCCTTGTTTTTAAGTGGCTAATGTAGAACCATGT40.12062726176115801No Hit
GGCACTAGAATGGCCCTCTTCCTCATCACTACATTATTTCAATTAAATAC40.12062726176115801No Hit
AAATACCGTTCAAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGAACCT40.12062726176115801No Hit
AAATACCGTTCAGAGCTGCCTTGTTTTTAGTGGCTAATGTAGAACCATGT40.12062726176115801No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)