FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008152464

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008152464
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3105
Total Bases450 kbp
Sequences flagged as poor quality0
Sequence length142-145
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAATACCGTTCAGAGCTGCCTTGTTTTTAAGTGGCTAATGTAGAACCATG59119.033816425120772No Hit
AAATACCGTTCAGAGCTGCCACCAAGAGCTTGCAAATTGCTGCTGGAACC54817.648953301127214No Hit
GGCACTAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATAC45214.557165861513688No Hit
GGCACTAGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATAC38612.431561996779388No Hit
AAATACCGTTCAGAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGAACC2568.244766505636072No Hit
AAATACCGTTCAGAACTGCCTTGTTTTTAAGTGGCTAATGTAGAACCATG1715.507246376811594No Hit
AAATACCGTTCAGCACTGCCTTGTTTTTAAGTGGCTAATGTAGAACCATG341.0950080515297906No Hit
AAATACCGTTCAGAGCTGCCTTGTTTTAAGTGGCTAATGTAGAACCATGT160.5152979066022545No Hit
GGCACTAGATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA150.4830917874396135No Hit
AAATACCGTTCAGAGCTGCCAGCTTGGGGTACCTGGGAGGGCACAGCACT110.35426731078904994No Hit
GGCACTAAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA100.322061191626409No Hit
AATACCGTTCAGAGCTGCCTTGTTTTTAAGTGGCTAATGTAGAACCATGT100.322061191626409No Hit
GGCACTGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA90.2898550724637681No Hit
AATACCGTTCAGAGCTGCCACCAAGAGCTTGCAAATTGCTGCTGGAACCT80.25764895330112725No Hit
GGCATAGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA70.22544283413848631No Hit
GGCACAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA60.1932367149758454No Hit
GGCACTAAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA60.1932367149758454No Hit
GGCACTAGATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA50.1610305958132045No Hit
AAATACGTTCAGAGCTGCCTTGTTTTTAAGTGGCTAATGTAGAACCATGT50.1610305958132045No Hit
GCACTAGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA50.1610305958132045No Hit
AAATACCGTCAGAGCTGCCACCAAGAGCTTGCAAATTGCTGCTGGAACCT50.1610305958132045No Hit
AAATACCGTTCAGAGCTGCCACCAAGAGCTTGCAAATTGCTGCTGGACCC50.1610305958132045No Hit
AAATACGTTCAGAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGAACCT40.12882447665056362No Hit
GGACTAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA40.12882447665056362No Hit
GGCACTAGAATGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA40.12882447665056362No Hit
GGCACTAGAATGGACCTCTTCGTTGATAGCACTGCCTTGTTTTTAAGTGG40.12882447665056362No Hit
AATACCGTTCAGAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGAACCT40.12882447665056362No Hit
AAATACCGTTCAGCGCTGCCTTGTTTTTAAGTGGCTAATGTAGAACCATG40.12882447665056362No Hit
GGCACTAGAATGGCCTCTTCCTCATCACTAAATTATTTCAATTAAATACA40.12882447665056362No Hit
AAAACCGTTCAGAGCTGCCACCAAGAGCTTGCAAATTGCTGCTGGAACCT40.12882447665056362No Hit
AAATACCGTTCGAGCTGCCACCAAGAGCTTGCAAATTGCTGCTGGAACCT40.12882447665056362No Hit
AAATACCGTTCAGAGCTGCCACCAAGAGCTTGCAAATTGATGCTGGAACC40.12882447665056362No Hit
AAATACCGTTCAGAGCTGCCACCAAGAGCTTGCAAATTGCTACTGGAACC40.12882447665056362No Hit
GGCACTAGAATGGCCCTCTTCCTCATCACTACATTATTTCAATTAAATAC40.12882447665056362No Hit
GGCATAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA40.12882447665056362No Hit
AAATACCGTTAGAGCTGCCTTGTTTTTAAGTGGCTAATGTAGAACCATGT40.12882447665056362No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)