FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008152474

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008152474
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10074
Total Bases1.4 Mbp
Sequences flagged as poor quality0
Sequence length142-145
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCACTAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATAC217521.590232281119714No Hit
AAATACCGTTCAGAGCTGCCACCAAGAGCTTGCAAATTGCTGCTGGAACC194119.267421083978558No Hit
GGCACTAGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATAC154315.316656740123088No Hit
AAATACCGTTCAGAGCTGCCTTGTTTTTAAGTGGCTAATGTAGAACCATG149314.820329561246773No Hit
AAATACCGTTCAGAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGAACC3743.712527297994838No Hit
AAATACCGTTCAGAACTGCCTTGTTTTTAAGTGGCTAATGTAGAACCATG3263.236053206273576No Hit
AAATACCGTTCAGCACTGCCTTGTTTTTAAGTGGCTAATGTAGAACCATG980.9728012705975779No Hit
GGCACTAGAATGGACCTCTTCGTTGATAGCACTGCCTTGTTTTTAAGTGG760.7544173118919992No Hit
AATACCGTTCAGAGCTGCCACCAAGAGCTTGCAAATTGCTGCTGGAACCT540.5360333531864204No Hit
AAATACCGTTCAGAGCTGCCAGCTTGGGGTACCTGGGAGGGCACAGCACT480.4764740917212627No Hit
AAATACCGTTCAGAGCTGCCTTGTTTTAAGTGGCTAATGTAGAACCATGT340.3375024816358944No Hit
GGCACTAGATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA330.3275759380583681No Hit
AAATACCGTTCAGAGCTGCCAGCTTGGGGTACCTGGGAGGGCATAGCACT330.3275759380583681No Hit
AATACCGTTCAGAGCTGCCTTGTTTTTAAGTGGCTAATGTAGAACCATGT280.2779432201707365No Hit
GGCACTAGAATGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA250.24816358943815764No Hit
GGCACTAGATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA250.24816358943815764No Hit
AAATACGTTCAGAGCTGCCTTGTTTTTAAGTGGCTAATGTAGAACCATGT210.2084574151280524No Hit
GGCACTAAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA200.19853087155052612No Hit
GGCACTGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA200.19853087155052612No Hit
GCACTAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA190.1886043279729998No Hit
GGACTAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA180.1786777843954735No Hit
GGCATAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA180.1786777843954735No Hit
GGCACTAGAATGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA170.1687512408179472No Hit
GGCACTAAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA170.1687512408179472No Hit
AAATACGTTCAGAGCTGCCACCAAGAGCTTGCAAATTGCTGCTGGAACCT150.14889815366289458No Hit
GGCACTAGAATGGACCTCTTCGTTGATACCACTGCTTACCCACAGAAACA140.13897161008536826Clontech SMARTer II A Oligonucleotide (95% over 22bp)
AAATACCGTTAGAGCTGCCACCAAGAGCTTGCAAATTGCTGCTGGAACCT140.13897161008536826No Hit
GCACTAGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA130.12904506650784198No Hit
AAATACCGTCAGAGCTGCCACCAAGAGCTTGCAAATTGCTGCTGGAACCT130.12904506650784198No Hit
GGCACTGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA130.12904506650784198No Hit
GGCACTAGAATGGACCTCTTCGTTGATACCACTGCCTTGTTTTTAAGTGG120.11911852293031568No Hit
AAATACCGTTCAGAGTGCCACCAAGAGCTTGCAAATTGCTGCTGGAACCT120.11911852293031568No Hit
AAATACCGTTCAGACTGCCTTGTTTTTAAGTGGCTAATGTAGAACCATGT110.10919197935278936No Hit
GGACTAGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA110.10919197935278936No Hit
GGCACAGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA110.10919197935278936No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)