FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008152502

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008152502
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8119
Total Bases1.1 Mbp
Sequences flagged as poor quality0
Sequence length143-145
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAATACCGTTCAGAGCTGCCACCAAGAGCTTGCAAATTGCTGCTGGAACC200524.695159502401772No Hit
AAATACCGTTCAGAGCTGCCTTGTTTTTAAGTGGCTAATGTAGAACCATG129515.950240177361744No Hit
GGCACTAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATAC121614.977213942603768No Hit
AAATACCGTTCAGAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGAACC6588.10444636038921No Hit
GGCACTAGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATAC5737.057519398940756No Hit
AAATACCGTTCAGAACTGCCTTGTTTTTAAGTGGCTAATGTAGAACCATG2913.5841852444882374No Hit
AAATACCGTTCAGAGCTGCCAGCTTGGGGTACCTGGGAGGGCACAGCACT1611.9830028328611897No Hit
AAATACCGTTCAGCACTGCCTTGTTTTTAAGTGGCTAATGTAGAACCATG1051.2932627170833846No Hit
AAATACCGTTCAGAGCTGCCAGCTTGGGGTACCTGGGAGGGCATAGCACT1031.2686291415198916No Hit
AATACCGTTCAGAGCTGCCACCAAGAGCTTGCAAATTGCTGCTGGAACCT470.5788890257420864No Hit
GGCACTAGAATGGACCTCTTCGTTGATAGCACTGCCTTGTTTTTAAGTGG460.56657223796034No Hit
AATACCGTTCAGAGCTGCCTTGTTTTTAAGTGGCTAATGTAGAACCATGT310.3818204212341421No Hit
AAATACCGTTCAGAGCTGCCTTGTTTTAAGTGGCTAATGTAGAACCATGT270.3325532701071561No Hit
GGCACTAGATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA260.3202364823254095No Hit
AATACCGTTCAGAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGAACCT180.22170218007143738No Hit
AAATACCGTTAGAGCTGCCACCAAGAGCTTGCAAATTGCTGCTGGAACCT170.20938539228969083No Hit
GGCACTGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA170.20938539228969083No Hit
GGCCTAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA140.17243502894445129No Hit
GGCACTAGAATGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA140.17243502894445129No Hit
AAATACGTTCAGAGCTGCCACCAAGAGCTTGCAAATTGCTGCTGGAACCT140.17243502894445129No Hit
GCACTAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA140.17243502894445129No Hit
AAATACCGTTCAGAGCTGCCACCAAGAGCTTGCAAATTGATGCTGGAACC130.16011824116270476No Hit
AAATACCGTTCAGAGCTGCCACCAAGAGCTTGCAAATTGCTGCTGGACCC130.16011824116270476No Hit
GGCATAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA130.16011824116270476No Hit
GGCACTAAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA120.14780145338095824No Hit
GGACTAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA110.1354846655992117No Hit
AAATACCGTTCAGAGCTGCCATTGCCCCACCCACTTCTCTGGTAGAGAAA110.1354846655992117No Hit
AAATACCGTTCAGACTGCCTTGTTTTTAAGTGGCTAATGTAGAACCATGT100.1231678778174652No Hit
AAATACCGTCAGAGCTGCCACCAAGAGCTTGCAAATTGCTGCTGGAACCT100.1231678778174652No Hit
GGCACTAGATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA100.1231678778174652No Hit
AAATACCTTCAGAGCTGCCACCAAGAGCTTGCAAATTGCTGCTGGAACCT100.1231678778174652No Hit
GGCACTAGAATGGACCTCTTCGTTGATACCACTGCTTACCCACAGAAACA90.11085109003571869Clontech SMARTer II A Oligonucleotide (95% over 22bp)
AAATACGTTCAGAGCTGCCTTGTTTTTAAGTGGCTAATGTAGAACCATGT90.11085109003571869No Hit
AAATACCGTTAGAGCTGCCTTGTTTTTAAGTGGCTAATGTAGAACCATGT90.11085109003571869No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)