FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008152508

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008152508
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7471
Total Bases1 Mbp
Sequences flagged as poor quality0
Sequence length143-145
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCACTAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATAC285738.24119930397537No Hit
AAATACCGTTCAGAGCTGCCTTGTTTTTAAGTGGCTAATGTAGAACCATG99313.291393387766028No Hit
AAATACCGTTCAGAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGAACC5647.549190202114844No Hit
GGCACTAGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATAC5026.719314683442645No Hit
AAATACCGTTCAGAGCTGCCACCAAGAGCTTGCAAATTGCTGCTGGAACC3334.45723464061036No Hit
AAATACCGTTCAGAGCTGCCACCAGGAGCTAGCAAATTGCTGCTGGAACC2653.547048587873109No Hit
AAATACCGTTCAGAACTGCCTTGTTTTTAAGTGGCTAATGTAGAACCATG2373.1722660955695354No Hit
AAATACCGTTCAGCACTGCCTTGTTTTTAAGTGGCTAATGTAGAACCATG650.870030785704725No Hit
GGCACTAGATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA530.7094097175746219No Hit
GGCACTAAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA340.4550930263686253No Hit
GGCACTAGAATGGACCTCTTCGTTGATAGCACTGCCTTGTTTTTAAGTGG310.4149377593360996No Hit
GGCACTGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA310.4149377593360996No Hit
GGCACTAGAATGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA290.38816758131441575No Hit
GGACTAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA260.34801231428189No Hit
GCACTAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA240.32124213626020615No Hit
GGCATAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA240.32124213626020615No Hit
AATACCGTTCAGAGCTGCCTTGTTTTTAAGTGGCTAATGTAGAACCATGT220.2944719582385223No Hit
AAATACCGTTCAGAGCTGCCAGCTTGGGGTACCTGGGAGGGCACAGCACT210.2810868692276804No Hit
AATACCGTTCAGAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGAACCT200.26770178021683844No Hit
GGCACTAGAATGGACCTCTTCGTCATCACTACATTATTTCAATTAAATAC180.24093160219515458No Hit
GGCCTAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA170.22754651318431265No Hit
GGCACAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA160.21416142417347073No Hit
AAATACCGTTCAGAGCTGCCTTGTTTTAAGTGGCTAATGTAGAACCATGT130.174006157140945No Hit
GGCACTAGAATGGACCTCTTCGTTGATACCACTGCCTTGTTTTTAAGTGG110.14723597911926115No Hit
AATACCGTTCAGAGCTGCCACCAGGAGCTAGCAAATTGCTGCTGGAACCT110.14723597911926115No Hit
GGCACTAGAATGGACCCTTCCTCATCACTACATTATTTCAATTAAATACA110.14723597911926115No Hit
AAATACCGTTCAGAGCTGCCAGCTTGGGGTACCTGGGAGGGCATAGCACT110.14723597911926115No Hit
GGCACTAGAATGGACCTCTTCCTCATCACTACATCATTTCAATTAAATAC110.14723597911926115No Hit
GGCACTAGAATGGACCTCTTCGTTGATACCACTGCTTACCCACAGAAACA100.13385089010841922Clontech SMARTer II A Oligonucleotide (95% over 22bp)
GCACTAGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA100.13385089010841922No Hit
GGCACTAGAAGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA100.13385089010841922No Hit
GGCACTAGAATGGCCTCTTCCTCATCACTACATTATTTCAATTAAATACA100.13385089010841922No Hit
AAATACCGTTAGAGCTGCCTTGTTTTTAAGTGGCTAATGTAGAACCATGT90.12046580109757729No Hit
GGCACTAGAATGGACCTCTCCTCATCACTACATTATTTCAATTAAATACA90.12046580109757729No Hit
GGCACTAGAATGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA80.10708071208673536No Hit
AAATACCGTTAGAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGAACCT80.10708071208673536No Hit
AAAACCGTTCAGAGCTGCCTTGTTTTTAAGTGGCTAATGTAGAACCATGT80.10708071208673536No Hit
AAATACCGTTCAGAGCTGCCTTGTTTTTAATGGCTAATGTAGAACCATGT80.10708071208673536No Hit
GGCACTAGAATGGCCCTCTTCCTCATCACTACATTATTTCAATTAAATAC80.10708071208673536No Hit
GGCACTAGAATGGACCTCTTCCTCATCAATACATTATTTCAATTAAATAC80.10708071208673536No Hit
GGCACTAGAATGGACCTCTTACTCATCACTACATTATTTCAATTAAATAC80.10708071208673536No Hit
AATACCGTTCAGAGCTGCCACCAAGAGCTTGCAAATTGCTGCTGGAACCT80.10708071208673536No Hit
GGCACTAGAATGGACCTCTTCCTCATCACTACATTATTTAAATTAAATAC80.10708071208673536No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)