FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008152515

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008152515
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5435
Total Bases788 kbp
Sequences flagged as poor quality0
Sequence length143-145
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTGTGTGTGTTTCTGTGGGTTTCTTTAAGGTTTGGACAGAAGGGTAAAG223941.195952161913524No Hit
ACTTAAAAACAAGGCAGTGCTAATGCCTAATGGTGCTACAGTTTCTGCCT132524.379024839006437No Hit
ACTTAAAAACAAGGCAGCTCTGAACGGTATTTTTGGACAGACCAAGTCTC83615.381784728610857No Hit
ACTTAAAAACAAGGCAGTTCTGAACGGTATTTTTGGACAGACCAAGTCTC1873.4406623735050603No Hit
ACTTAAAAACAAGGCAGTGCTGAACGGTATTTTTGGACAGACCAAGTCTC711.3063477460901565No Hit
ACTTAAAACAAGGCAGTGCTAATGCCTAATGGTGCTACAGTTTCTGCCTC360.6623735050597976No Hit
ACTTAAAAACAAGGCAGTGCTATCAACGAAGAGGTCCATTCTAGTGCCTT190.34958601655933763No Hit
ACTTAAAAACAAGGCAGTGCTGTGCCCTCCCAGGTACCCCAAGCTGGCAG170.31278748850045995No Hit
ACTAAAAACAAGGCAGTGCTAATGCCTAATGGTGCTACAGTTTCTGCCTC140.2575896964121435No Hit
TGTGTGTGTGTTCTGTGGGTTTCTTTAAGGTTTGGACAGAAGGGTAAAGC140.2575896964121435No Hit
TGTGTGTGTGTTTTGTGGGTTTCTTTAAGGTTTGGACAGAAGGGTAAAGC120.22079116835326587No Hit
TGTGTGTGTGTTTCTGTGGGTTTCTTTAAGGTTTGGACAGGAGGGTAAAG110.20239190432382706No Hit
ACTTAAAAACAAGGCAGTGCTATGCCCTCCCAGGTACCCCAAGCTGGCAG110.20239190432382706No Hit
ACTTAAAAACAAGGCAGCTCTGAACGGTATTTTGGACAGACCAAGTCTCT110.20239190432382706No Hit
ACTTAAAAACAAGGCAGCTCTAACGGTATTTTTGGACAGACCAAGTCTCT100.18399264029438822No Hit
ATTAAAAACAAGGCAGTGCTAATGCCTAATGGTGCTACAGTTTCTGCCTC100.18399264029438822No Hit
TGTGTGTGTGTTTCTGTGGGTTTCTTTAAGGTTTGGACAGAAGGGTGAAG90.1655933762649494No Hit
ACTTAAAACAAGGCAGCTCTGAACGGTATTTTTGGACAGACCAAGTCTCT90.1655933762649494No Hit
TGTGTGTGTGTTTCTGTGGGTTTCTTTAAGGTTTGGACAGAAGGGTAAGG80.14719411223551057No Hit
ACTTAAAAACAAGGCAGTGGTATCAACGAAGAGGTCCATTCTAGTGCCTT80.14719411223551057No Hit
ACTTAAAAACAAGGCAGCTCTGACGGTATTTTTGGACAGACCAAGTCTCT80.14719411223551057No Hit
TGTGTGTGTGTTTCTGTGGGTTTCTTTAAGGTTTGGACAGAAGGGTAGAG70.12879484820607176No Hit
TGTGTGTGTGTTTCTGTGGGTTTCTCTAAGGTTTGGACAGAAGGGTAAAG70.12879484820607176No Hit
TGTGTGTGTTTCTGTGGGTTTCTTTAAGGTTTGGACAGAAGGGTAAAGCT70.12879484820607176No Hit
ACTTAAAAACAAGGCAGCTCTGAACGTATTTTTGGACAGACCAAGTCTCT70.12879484820607176No Hit
TGTGTGTGGTTTCTGTGGGTTTCTTTAAGGTTTGGACAGAAGGGTAAAGC70.12879484820607176No Hit
ACTTAAAAACAAGGCAGTGCTAATGCCTAATGGCGCTACAGTTTCTGCCT70.12879484820607176No Hit
ACTTAAAAACAAGGCGGTGCTAATGCCTAATGGTGCTACAGTTTCTGCCT70.12879484820607176No Hit
ACTTAAAAAAAGGCAGTGCTAATGCCTAATGGTGCTACAGTTTCTGCCTC70.12879484820607176No Hit
TGTGTGTGTGTTTCTGTGGTTTCTTTAAGGTTTGGACAGAAGGGTAAAGC70.12879484820607176No Hit
ACTTAAAAACAAGGCAGTGCTAATGACTAATGGTGCTACAGTTTCTGCCT60.11039558417663294No Hit
ACTTAAAAACAGGCAGTGCTAATGCCTAATGGTGCTACAGTTTCTGCCTC60.11039558417663294No Hit
ACTAAAAACAAGGCAGCTCTGAACGGTATTTTTGGACAGACCAAGTCTCT60.11039558417663294No Hit
ACTTAAAACAAGGCAGTTCTGAACGGTATTTTTGGACAGACCAAGTCTCT60.11039558417663294No Hit
ACTTAAAAACAAGGCAGTGCTAATGTCTAATGGTGCTACAGTTTCTGCCT60.11039558417663294No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)