FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008152518

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008152518
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9261
Total Bases1.3 Mbp
Sequences flagged as poor quality0
Sequence length143-145
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCACTAGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATAC218823.62595831983587No Hit
GGCACTAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATAC144215.570672713529857No Hit
AAATACCGTTCAGAGCTGCCTTGTTTTTAAGTGGCTAATGTAGAACCATG138014.901198574667962No Hit
AAATACCGTTCAGAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGAACC104211.251484720872476No Hit
AAATACCGTTCAGAGCTGCCACCAAGAGCTTGCAAATTGCTGCTGGAACC7998.627578015333118No Hit
AAATACCGTTCAGAACTGCCTTGTTTTTAAGTGGCTAATGTAGAACCATG3663.952057013281503No Hit
AAATACCGTTCAGCACTGCCTTGTTTTTAAGTGGCTAATGTAGAACCATG830.8962315084764064No Hit
AAATACCGTTCAGAGCTGCCAGCTTGGGGTACCTGGGAGGGCACAGCACT600.6478781988986071No Hit
GGCACTAGAATGGACCTCTTCGTTGATAGCACTGCCTTGTTTTTAAGTGG370.39952488932080765No Hit
AAATACCGTTCAGAGCTGCCTTGTTTTAAGTGGCTAATGTAGAACCATGT310.334737069430947No Hit
GGCACTAGATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA310.334737069430947No Hit
GGCACTAGATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA280.30234315948601664No Hit
AATACCGTTCAGAGCTGCCTTGTTTTTAAGTGGCTAATGTAGAACCATGT280.30234315948601664No Hit
GGCACTAAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA270.2915451895043732No Hit
AATACCGTTCAGAGCTGCCACCAAGAGCTTGCAAATTGCTGCTGGAACCT240.25915127955944284No Hit
GGCACTAGAATGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA230.24835330957779936No Hit
AAATACCGTTCAGAGCTGCCAGCTTGGGGTACCTGGGAGGGCATAGCACT230.24835330957779936No Hit
GGCATAGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA210.22675736961451248No Hit
GGCACTGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA210.22675736961451248No Hit
GCACTAGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA190.20516142965122555No Hit
AATACCGTTCAGAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGAACCT190.20516142965122555No Hit
GGACTAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA180.1943634596695821No Hit
GGCACAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA170.18356548968793868No Hit
GGCACTAGAATGGACCTCTTCGTCATCACTAAATTATTTCAATTAAATAC160.17276751970629523No Hit
GGCACTAAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA160.17276751970629523No Hit
AAATACGTTCAGAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGAACCT150.16196954972465177No Hit
GGCACTAGAATGGACCTCTTCGTTGATACCACTGCTTACCCACAGAAACA150.16196954972465177Clontech SMARTer II A Oligonucleotide (95% over 22bp)
GGCACTGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA150.16196954972465177No Hit
GCACTAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA150.16196954972465177No Hit
GGACTAGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA140.15117157974300832No Hit
GGCCTAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA130.14037360976136484No Hit
AAATACGTTCAGAGCTGCCTTGTTTTTAAGTGGCTAATGTAGAACCATGT130.14037360976136484No Hit
GGCACTAGAATGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA130.14037360976136484No Hit
GGCACTAGAATGGACCTCTTCGTTGATACCACTGCCTTGTTTTTAAGTGG120.12957563977972142No Hit
GGCACAGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA120.12957563977972142No Hit
GGCACTAGAATGGACCTCTCCCTCATCACTAAATTATTTCAATTAAATAC110.11877766979807797No Hit
GGCATAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA110.11877766979807797No Hit
GGCACTAGAATGGACCTCTTCGTCATCACTACATTATTTCAATTAAATAC100.1079796998164345No Hit
AAATACCGTTCAGAGCTGCCTTGTTTTTAGTGGCTAATGTAGAACCATGT100.1079796998164345No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)