FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008152519

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008152519
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4312
Total Bases625.2 kbp
Sequences flagged as poor quality0
Sequence length143-145
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTGTGTGTGTTTCTGTGGGTTTCTTTAAGGTTTGGACAGAAGGGTAAAG165338.33487940630798No Hit
ACTTAAAAACAAGGCAGTGCTAATGCCTAATGGTGCTACAGTTTCTGCCT116226.948051948051948No Hit
ACTTAAAAACAAGGCAGCTCTGAACGGTATTTTTGGACAGACCAAGTCTC65415.166975881261596No Hit
ACTTAAAAACAAGGCAGTTCTGAACGGTATTTTTGGACAGACCAAGTCTC1774.10482374768089No Hit
ACTTAAAAACAAGGCAGTGCTGAACGGTATTTTTGGACAGACCAAGTCTC370.8580705009276438No Hit
ACTTAAAACAAGGCAGTGCTAATGCCTAATGGTGCTACAGTTTCTGCCTC220.5102040816326531No Hit
ACTTAAAAACAAGGCAGTGCTGTGCCCTCCCAGGTACCCCAAGCTGGCAG160.3710575139146568No Hit
ACTTAAAAACAGGCAGTGCTAATGCCTAATGGTGCTACAGTTTCTGCCTC160.3710575139146568No Hit
ACTTAAAAACAAGGCAGCTCTGAACGGTATTTTGGACAGACCAAGTCTCT150.34786641929499074No Hit
ACTTAAAAACAAGGCAGTGCTATCAACGAAGAGGTCCATTCTAGTGCCTT140.3246753246753247No Hit
ACTTAAAACAAGGCAGCTCTGAACGGTATTTTTGGACAGACCAAGTCTCT110.25510204081632654No Hit
ACTTAAAAACAAGGCAGTGCTATGCCCTCCCAGGTACCCCAAGCTGGCAG110.25510204081632654No Hit
TGTGTGTGTGTTTCTGTGGGTTTCTTTAAGGTTTGGACAGAAGGGTAAGG90.20871985157699444No Hit
TGTGTGTGTGTTCTGTGGGTTTCTTTAAGGTTTGGACAGAAGGGTAAAGC90.20871985157699444No Hit
TGTGTGTGTGTTTTGTGGGTTTCTTTAAGGTTTGGACAGAAGGGTAAAGC80.1855287569573284No Hit
ACTAAAAACAAGGCAGTGCTAATGCCTAATGGTGCTACAGTTTCTGCCTC80.1855287569573284No Hit
TGTGTGTGTGTTTCTGTGGGTTTCTTTAAGGTTTGGACAGGAGGGTAAAG70.16233766233766234No Hit
ACTTAAAAACAAGGCAGCTCTGAACGGTATTTTTGGACAGCCCAAGTCTC70.16233766233766234No Hit
CCTTAAAAACAAGGCAGTGCTAATGCCTAATGGTGCTACAGTTTCTGCCT60.13914656771799627No Hit
TGTGTGTGTGTTTCTGTGGGTTTCCTTAAGGTTTGGACAGAAGGGTAAAG60.13914656771799627No Hit
ACTTAAAAACAAGGCAGCTCGAACGGTATTTTTGGACAGACCAAGTCTCT60.13914656771799627No Hit
TGTGTGTGTGTTTCTGTGGGTTTCTTTAAGGTTTGGACAGAGGGGTAAAG60.13914656771799627No Hit
TGTGTGTGTTTTCTGTGGGTTTCTTTAAGGTTTGGACAGAAGGGTAAAGC60.13914656771799627No Hit
TGTGTGTGTGTTTCTGTGGGTTTCTTTAAGGTTTGGGCAGAAGGGTAAAG60.13914656771799627No Hit
ACTTAAAAACAAGGCAGTGCTAATGACTAATGGTGCTACAGTTTCTGCCT50.11595547309833025No Hit
TGTGTGTGTGTTTCTGTGGGTTTCTTTAAGGTTTGGACAGAAGGGTGAAG50.11595547309833025No Hit
ACTTAAAAACAAGGCAGTTCTGAACGGTATTTTGGACAGACCAAGTCTCT50.11595547309833025No Hit
TGTGTGTGTTTCTGTGGGTTTCTTTAAGGTTTGGACAGAAGGGTAAAGCT50.11595547309833025No Hit
TGTTGTGTGTTTCTGTGGGTTTCTTTAAGGTTTGGACAGAAGGGTAAAGC50.11595547309833025No Hit
ACTTAAAAACAAGGCAGCTCTGACGGTATTTTTGGACAGACCAAGTCTCT50.11595547309833025No Hit
TGTGTGTGTGTTTCTGTGGTTTCTTTAAGGTTTGGACAGAAGGGTAAAGC50.11595547309833025No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)