FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008152556

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008152556
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2137
Total Bases309.7 kbp
Sequences flagged as poor quality0
Sequence length143-145
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCACTAGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATAC57927.094057089377632No Hit
AAATACCGTTCAGAGCTGCCTTGTTTTTAAGTGGCTAATGTAGAACCATG37317.454375292466075No Hit
AAATACCGTTCAGAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGAACC32915.395414131960692No Hit
AAATACCGTTCAGAGCTGCCACCAAGAGCTTGCAAATTGCTGCTGGAACC21610.107627515208236No Hit
AAATACCGTTCAGAACTGCCTTGTTTTTAAGTGGCTAATGTAGAACCATG1095.100608329433785No Hit
AAATACCGTTCAGAGCTGCCAGCTTGGGGTACCTGGGAGGGCACAGCACT341.5910154422087037No Hit
AAATACCGTTCAGCACTGCCTTGTTTTTAAGTGGCTAATGTAGAACCATG321.4974262985493683No Hit
GGCACTAGATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA140.6551240056153487No Hit
GGCACTAAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA110.5147402901263454No Hit
AAATACCGTTCAGAGCTGCCTTGTTTTAAGTGGCTAATGTAGAACCATGT110.5147402901263454No Hit
GGCACTAGAATGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA90.42115114646700985No Hit
AAATACCGTTCAGAGCTGCCAGCTTGGGGTACCTGGGAGGGCATAGCACT90.42115114646700985No Hit
GCACTAGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA80.37435657463734207No Hit
GGCACAGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA80.37435657463734207No Hit
AATACCGTTCAGAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGAACCT70.32756200280767434No Hit
GGACTAGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA70.32756200280767434No Hit
AATACCGTTCAGAGCTGCCTTGTTTTTAAGTGGCTAATGTAGAACCATGT70.32756200280767434No Hit
GGCATAGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA50.2339728591483388No Hit
AATACCGTTCAGAGCTGCCACCAAGAGCTTGCAAATTGCTGCTGGAACCT50.2339728591483388No Hit
AAATACCGTTCAGAACTGCCTTGTTTTAAGTGGCTAATGTAGAACCATGT50.2339728591483388No Hit
AAATACGTTCAGAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGAACCT40.18717828731867103No Hit
AAATACGTTCAGAGCTGCCTTGTTTTTAAGTGGCTAATGTAGAACCATGT40.18717828731867103No Hit
AAATACCGTTCAGGCTGCCACCAAGAGCTTGCAAATTGCTGCTGGAACCT40.18717828731867103No Hit
AAAACCGTTCAGAGCTGCCTTGTTTTTAAGTGGCTAATGTAGAACCATGT40.18717828731867103No Hit
GGCACTGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA40.18717828731867103No Hit
AAATACCGTTCAGAGTGCCACCAAGAGCTTGCAAATTGCTGCTGGAACCT30.14038371548900327No Hit
AAATACCGTCAGAGCTGCCTTGTTTTTAAGTGGCTAATGTAGAACCATGT30.14038371548900327No Hit
GGCACTAGAATGGACTTCTTCCTCATCACTAAATTATTTCAATTAAATAC30.14038371548900327No Hit
GGCACTAGAATGGACCTCTTCGTTGATACCACTGCTTACCCACAGAAACA30.14038371548900327Clontech SMARTer II A Oligonucleotide (95% over 22bp)
GGCACTAGAATGGACCTCTTCGTCATCACTAAATTATTTCAATTAAATAC30.14038371548900327No Hit
AATACCGTTCAGAACTGCCTTGTTTTTAAGTGGCTAATGTAGAACCATGT30.14038371548900327No Hit
AAATACCGTTCAGCGCTGCCTTGTTTTTAAGTGGCTAATGTAGAACCATG30.14038371548900327No Hit
GGCACTAGAATGGACCTCTTCCCCATCACTAAATTATTTCAATTAAATAC30.14038371548900327No Hit
GGCCTAGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA30.14038371548900327No Hit
AAATACCGTCAGAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGAACCT30.14038371548900327No Hit
AAATACCGTTCAGACTGCCTTGTTTTTAAGTGGCTAATGTAGAACCATGT30.14038371548900327No Hit
AAATACCGTTCAGAGCTGCCACCAGGAGCTTGCAAATTTCTGCTGGAACC30.14038371548900327No Hit
GGCACTAGAATGGACCTCTTCCTCATCACTAAATTAATTCAATTAAATAC30.14038371548900327No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)